##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630155.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	89819
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83882029414712	31.0	31.0	34.0	30.0	34.0
2	31.983199545753127	33.0	31.0	34.0	30.0	34.0
3	32.0654538572017	33.0	31.0	34.0	30.0	34.0
4	35.76277847671428	37.0	35.0	37.0	35.0	37.0
5	35.70518487179773	37.0	35.0	37.0	35.0	37.0
6	35.79759293690645	37.0	35.0	37.0	35.0	37.0
7	35.78120442222692	37.0	35.0	37.0	35.0	37.0
8	35.78231777240896	37.0	35.0	37.0	35.0	37.0
9	37.44900299491199	39.0	37.0	39.0	35.0	39.0
10	37.38053195871697	39.0	37.0	39.0	34.0	39.0
11	37.473107026353	39.0	37.0	39.0	35.0	39.0
12	37.36002404836393	39.0	37.0	39.0	34.0	39.0
13	37.4193767465681	39.0	37.0	39.0	34.0	39.0
14	38.52777252029081	40.0	38.0	41.0	34.0	41.0
15	38.51353277146261	40.0	38.0	41.0	34.0	41.0
16	38.42276133112148	40.0	38.0	41.0	34.0	41.0
17	38.47144813458177	40.0	38.0	41.0	34.0	41.0
18	38.50642959730124	40.0	38.0	41.0	34.0	41.0
19	38.510326322938354	40.0	38.0	41.0	34.0	41.0
20	38.524588338770194	40.0	38.0	41.0	34.0	41.0
21	38.48761397922488	40.0	38.0	41.0	34.0	41.0
22	38.4316458655741	40.0	38.0	41.0	34.0	41.0
23	38.37460893574856	40.0	38.0	41.0	34.0	41.0
24	38.34416994177179	40.0	38.0	41.0	34.0	41.0
25	38.27773633640989	40.0	38.0	41.0	34.0	41.0
26	38.06381723243412	40.0	37.0	41.0	33.0	41.0
27	37.925271935781964	40.0	37.0	41.0	33.0	41.0
28	37.85697903561607	40.0	37.0	41.0	33.0	41.0
29	37.76498291007471	40.0	37.0	41.0	33.0	41.0
30	37.60320199512353	40.0	37.0	41.0	33.0	41.0
31	37.45606163506608	39.0	36.0	41.0	32.0	41.0
32	37.30059341564702	39.0	36.0	41.0	32.0	41.0
33	37.21788263062381	39.0	35.0	41.0	32.0	41.0
34	37.07243456284305	39.0	35.0	41.0	31.0	41.0
35	36.97156503635088	39.0	35.0	41.0	31.0	41.0
36	36.76159832552133	39.0	35.0	41.0	31.0	41.0
37	36.663912980549775	39.0	35.0	41.0	30.0	41.0
38	36.510604660483864	39.0	35.0	41.0	30.0	41.0
39	36.34434807780091	39.0	35.0	40.0	30.0	41.0
40	36.14806444070854	39.0	35.0	40.0	30.0	41.0
41	35.94350861176366	38.0	35.0	40.0	29.0	41.0
42	35.80602099778444	38.0	35.0	40.0	28.0	41.0
43	34.5369687927944	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	2.0
19	7.0
20	14.0
21	28.0
22	62.0
23	109.0
24	166.0
25	264.0
26	404.0
27	631.0
28	827.0
29	1200.0
30	1577.0
31	2076.0
32	2595.0
33	3323.0
34	4422.0
35	6019.0
36	8315.0
37	12974.0
38	20455.0
39	24344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.37257150491544	19.61389015687104	13.06627773633641	22.947260601877108
2	20.200625702802302	21.76376935837629	33.27024348968481	24.7653614491366
3	22.33825805230519	23.38814727396208	30.386666518219975	23.886928155512756
4	15.117068771640744	17.242454269141273	34.157583584764915	33.48289337445306
5	14.89328538505216	37.093488014785294	32.706888297576235	15.306338302586312
6	31.870762310869637	33.70111001013149	17.122212449481736	17.30591522951714
7	26.77941192843385	32.12571950255514	20.36874158028925	20.726126988721763
8	28.92038432848284	31.317427270399357	20.254066511539875	19.50812188957793
9	26.917467351005914	12.958282768679233	20.81742170364845	39.30682817666641
10	20.93543682294392	25.921018938086597	29.07068660305726	24.07285763591222
11	34.714258675781295	22.71011701310413	19.067235217492957	23.508389093621616
12	20.831895256014874	27.353900622362755	27.911689063561163	23.902515058061212
13	33.33815785078881	18.832318329084046	24.92902392589541	22.900499894231732
14	23.60191050891237	21.985326044600807	25.670515147129226	28.7422482993576
15	30.0103541566929	24.72750754294748	22.58764849308053	22.674489807279084
16	23.49057549070909	25.151693962301962	25.638227991850275	25.719502555138668
17	21.89180462931006	26.986495062291944	25.467885413999262	25.653814894398735
18	22.054353755886837	23.5417895990826	28.01857068103631	26.385285963994253
19	22.79473162693862	25.878711631169356	27.955109720660438	23.371447021231585
20	24.728620893129516	23.02408176443737	29.478172769681247	22.769124572751867
21	25.441165009630478	23.771139736581347	27.165744441599216	23.621950812188956
22	25.83083757334194	24.553824914550372	26.50107438292566	23.11426312918202
23	25.167280864850422	24.40686269052205	26.908560549549648	23.51729589507788
24	23.94593571516049	25.089346352108127	27.18689809505784	23.777819837673544
25	24.31779467595943	25.287522684509963	27.248132355069636	23.14655028446097
26	25.597034035115062	24.505950856722965	26.518887985838184	23.378127122323786
27	24.222046560304612	24.839955911332794	27.550963604582552	23.387033923780045
28	23.038555316803794	26.128102071944685	27.29711976307908	23.536222848172436
29	23.56628330308732	25.624867789665885	26.985381712109913	23.823467195136885
30	23.073069172446807	27.288212961622822	27.949542969750276	21.6891748961801
31	23.979336220621473	25.450071811086737	26.80056558189247	23.770026386399316
32	21.963059040960154	26.036807357018006	27.00319531502243	24.99693828699941
33	22.946147251695077	25.451185161268775	28.433850298934527	23.168817288101625
34	21.370756744118726	25.821930771885683	28.107638695598926	24.699673788396666
35	22.791391576392524	26.299557999977736	27.87717520791815	23.031875215711597
36	22.126721517718966	26.89186029681916	27.333860319086163	23.647557866375713
37	22.563154789075806	27.10339683140538	26.788318729890115	23.5451296496287
38	22.027633351518052	26.1759761297721	27.283759560894687	24.512630957815162
39	22.550907937073447	24.999164987363475	29.059553101236936	23.390373974326145
40	21.73593560382547	25.820817421703644	29.26663623509502	23.176610739375857
41	21.25051492445919	24.75088789677017	29.481512820227344	24.517084358543293
42	20.942116924036117	25.113840056112853	29.64406194680413	24.299981073046904
43	21.00001113350182	25.878711631169356	29.2521626827286	23.86911455260023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	6.5
2	12.0
3	56.0
4	100.0
5	100.0
6	145.5
7	191.0
8	181.0
9	171.0
10	231.0
11	291.0
12	291.0
13	554.5
14	818.0
15	1317.5
16	1817.0
17	1511.0
18	1205.0
19	1205.0
20	1443.5
21	1682.0
22	1163.5
23	645.0
24	734.5
25	824.0
26	824.0
27	905.5
28	987.0
29	1027.5
30	1068.0
31	1290.0
32	1512.0
33	1512.0
34	1789.0
35	2066.0
36	2216.0
37	2366.0
38	2736.0
39	3106.0
40	3106.0
41	3640.5
42	4175.0
43	4892.0
44	5609.0
45	6500.5
46	7392.0
47	7392.0
48	8570.5
49	9749.0
50	8931.0
51	8113.0
52	7932.5
53	7752.0
54	7752.0
55	7432.5
56	7113.0
57	6788.5
58	6464.0
59	5575.5
60	4687.0
61	4687.0
62	3940.0
63	3193.0
64	2645.0
65	2097.0
66	1812.0
67	1527.0
68	1527.0
69	1341.0
70	1155.0
71	953.0
72	751.0
73	618.5
74	486.0
75	486.0
76	414.5
77	343.0
78	267.5
79	192.0
80	135.5
81	79.0
82	79.0
83	62.5
84	46.0
85	35.0
86	24.0
87	13.5
88	3.0
89	3.0
90	3.5
91	4.0
92	3.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	89819.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.60955922466294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5093604945333	39.73324129638495
2	10.247046659480388	10.371970295817142
3	3.7375981696988365	5.674745877820951
4	1.9072970059616783	3.861098431289593
5	1.12853905889082	2.855743216914016
6	0.7853575906901028	2.3847960899141607
7	0.5741689948742768	2.034090782573843
8	0.4597751721407044	1.8615215043587658
9	0.39377873594825874	1.793607143254768
>10	2.074487977649207	19.14962313096338
>50	0.13419275359130606	4.493481334684199
>100	0.04179774292188222	3.073959852592436
>500	0.006599643619244561	2.7121210434317904
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	988	1.0999899798483617	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	867	0.9652746078223983	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	581	0.6468564557610306	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	313	0.3484786069762522	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	206	0.2293501374987475	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	192	0.21376323495028893	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	156	0.17368262839710974	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	155	0.172569278215077	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	155	0.172569278215077	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	150	0.16700252730491322	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	134	0.14918892439238915	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	131	0.14584887384629086	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	130	0.14473552366425813	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	126	0.1402821229361271	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	123	0.13694207239002884	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	120	0.13360202184393058	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	115	0.1280352709337668	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	113	0.1258085705697013	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	112	0.12469522038766852	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	112	0.12469522038766852	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.12358187020563578	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.11912846947750476	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	100	0.11133501820327547	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	97	0.10799496765717721	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCG	95	0.1057682672931117	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	93	0.10354156692904619	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCT	92	0.10242821674701344	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	91	0.10131486656498069	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	90	0.10020151638294793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.004453400728131019	0.0
17	0.0	0.0	0.0	0.005566750910163774	0.0
18	0.0	0.0	0.0	0.005566750910163774	0.0
19	0.0	0.0	0.0	0.006680101092196529	0.0
20	0.0	0.0	0.0	0.006680101092196529	0.0
21	0.0	0.0	0.0	0.008906801456262037	0.0
22	0.0	0.0	0.0	0.014473552366425812	0.0
23	0.0	0.0	0.0	0.02115365345862234	0.0
24	0.0	0.0	0.0	0.040080606553179174	0.0
25	0.0	0.0	0.0	0.040080606553179174	0.0
26	0.0	0.0	0.0	0.043420657099277435	0.0
27	0.0	0.0	0.0	0.05455415891960498	0.0
28	0.0	0.0	0.0	0.08016121310635835	0.0
29	0.0	0.0	0.0	0.12358187020563578	0.0
30	0.0	0.0	0.0	0.21376323495028893	0.0
31	0.0	0.0	0.0	0.4453400728131019	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAGAT	30	3.5733476E-4	30.833334	16
CAGATAA	30	3.5733476E-4	30.833334	18
TCAGATA	30	3.5733476E-4	30.833334	17
CTATGTA	25	0.0054697352	29.6	9
AGATAAC	25	0.0054697352	29.6	19
CGTTACC	25	0.0054697352	29.6	2
ACGATGA	25	0.0054697352	29.6	24
ACAGGGC	25	0.0054697352	29.6	34
CCTATGT	25	0.0054697352	29.6	8
GACCTTA	25	0.0054697352	29.6	29
AACAGGG	25	0.0054697352	29.6	33
TCAACAA	25	0.0054697352	29.6	18
ATAGAGC	35	8.805615E-4	26.428572	37
TATGTAT	35	8.805615E-4	26.428572	10
ATGTATT	40	0.0019174316	23.125002	11
GAGGCCA	40	0.0019174316	23.125002	9
ACTAACA	60	3.6772908E-5	21.583334	33
CCACTAA	70	5.0160634E-6	21.142859	31
TCGCGCG	70	5.0160634E-6	21.142859	9
CCTTATT	45	0.003798817	20.555557	27
>>END_MODULE