FastQCFastQC Report
Fri 10 Feb 2017
ERR1630154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526405
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90910.3598393765053505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90540.35837484488828986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68460.27097793109180834No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG60950.24125189745903766No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG60290.2386394897096863No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC55490.21964016062349465No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG54780.21682984319616214No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT54210.21457367286717688No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA41040.16244426368693857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39450.15615073592713757No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC38450.1521925423675143No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT38080.15072801075045372No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA37640.1489864055842195No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT36600.1448698842822113No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG36280.14360326234313184No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA35670.14118876427176164No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC35640.14107001846497294No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC34960.13837844684442915No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA33940.13434108941361342No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC33680.13331195908811136No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG32980.1305412235963751No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG32590.128997528108122No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG32340.1280079797182162No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA32190.1274142506842727No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA30210.11957702743621865No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC28000.11082941966945126No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC26910.1065149886894619No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC26420.1045754738452465No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT25760.10196306609589516No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG25620.1014089189975479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG656.906317E-519.9230779
TATACCG759.272982E-619.7333345
ACGGACC4400.016.8181828
AAGACGG4950.016.4444435
CGGACCA4550.016.2637379
CAAGACG5050.015.7524754
GACGGAC4600.015.6847837
TCTATAC1601.0981239E-815.031253
GTCGTAA1001.0941874E-414.820
GGTATCA43150.014.53418351
CTTATAC9750.014.42051237
TCTATCG908.2788523E-414.38888831
CGAGCCG5300.014.31132115
ATACCGC5300.014.31132127
TACCGCA5300.014.31132128
ACACCGT1304.4487515E-614.2307696
CGCCAAT1051.6568702E-414.09523914
GCGCAAG5700.013.956141
GCGTTAT4000.013.8750011
CGTAAAC800.00630137613.87522
Produced by FastQC (version 0.11.5)