FastQCFastQC Report
Fri 10 Feb 2017
ERR1630153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127436
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC57710.511869409882246No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT31210.27682280856740427No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG23410.20763928063322443No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG22040.19548781482940053No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT21560.1912303669565279No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG19290.17109618639106786No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG18410.16329086529080142No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT18360.1628473811373772No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT17160.15220376145519568No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT17080.1514941868097169No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG16260.14422104669355953No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG15990.14182623226506869No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG15730.13952011466726272No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC15530.1377461780535658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.13499657630233558No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT14820.13144870307494175No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG14660.13002955378398418No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC14490.1285217076623418No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG13870.12302250415988135No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT13360.11849896579495421No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT12920.11459630524482099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12690.11255627813906953No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT12340.10945188906509992No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC11990.1063474999911303No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA11810.10475095703880309No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11510.10209005211825771No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC11400.10111438698072439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT250.005496299429.66
TGCATCA13100.021.32442714
ATACACT705.1017814E-621.1428574
CAGGACA14850.020.8047144
CCGGTTA450.003825763220.55555527
GACAGGC14050.020.4092527
GCTGCAT14050.020.14590812
ACAGGCT14150.020.1342758
CCAGGAC14650.020.0784973
GCATCAG13950.020.02509115
CTGCATC14600.019.38698813
TCCAGGA15950.018.9059562
CGCAAGA3050.018.8032782
TTTAGCG500.007034800518.526
AAGACGG3000.018.55
ATCAGAA15300.018.37908617
AGAGGCC15500.018.14193723
TCTGTAC1156.40448E-817.6956523
GTATAGA1156.40448E-817.6956521
TTATTGT1156.40448E-817.6956524
Produced by FastQC (version 0.11.5)