##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630153.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1127436
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.821104701286814	31.0	31.0	34.0	30.0	34.0
2	32.02159945220837	33.0	31.0	34.0	30.0	34.0
3	32.15634590344818	34.0	31.0	34.0	30.0	34.0
4	35.81335259828496	37.0	35.0	37.0	35.0	37.0
5	35.74280757399976	37.0	35.0	37.0	35.0	37.0
6	35.79243256379963	37.0	35.0	37.0	35.0	37.0
7	35.76623240698363	37.0	35.0	37.0	35.0	37.0
8	35.74000741505505	37.0	35.0	37.0	33.0	37.0
9	37.46127762462792	39.0	37.0	39.0	35.0	39.0
10	37.37235284308821	39.0	37.0	39.0	34.0	39.0
11	37.45006191038782	39.0	37.0	39.0	35.0	39.0
12	37.32196151267123	39.0	37.0	39.0	34.0	39.0
13	37.34360797419986	39.0	37.0	39.0	34.0	39.0
14	38.56322221394385	40.0	38.0	41.0	34.0	41.0
15	38.55208100504153	40.0	38.0	41.0	34.0	41.0
16	38.54018321217346	40.0	38.0	41.0	34.0	41.0
17	38.49614612270674	40.0	38.0	41.0	34.0	41.0
18	38.40335061147595	40.0	38.0	41.0	34.0	41.0
19	38.40635654706786	40.0	38.0	41.0	34.0	41.0
20	38.34884640902011	40.0	38.0	41.0	34.0	41.0
21	38.31058170929436	40.0	38.0	41.0	34.0	41.0
22	38.25683054293104	40.0	38.0	41.0	34.0	41.0
23	38.19314976637255	40.0	38.0	41.0	33.0	41.0
24	38.16062729946533	40.0	38.0	41.0	33.0	41.0
25	38.12146410084475	40.0	38.0	41.0	33.0	41.0
26	37.92266878119911	40.0	37.0	41.0	33.0	41.0
27	37.83211463887972	40.0	37.0	41.0	33.0	41.0
28	37.697914560116935	40.0	37.0	41.0	32.0	41.0
29	37.618057255578144	40.0	37.0	41.0	32.0	41.0
30	37.551924898619525	40.0	37.0	41.0	32.0	41.0
31	37.467160885407246	40.0	37.0	41.0	32.0	41.0
32	37.36723148808447	40.0	37.0	41.0	31.0	41.0
33	37.28108823915504	40.0	36.0	41.0	31.0	41.0
34	37.17564189896367	39.0	36.0	41.0	31.0	41.0
35	37.03372963077283	39.0	36.0	41.0	31.0	41.0
36	36.9177203850152	39.0	36.0	41.0	30.0	41.0
37	36.83507267818306	39.0	35.0	41.0	30.0	41.0
38	36.7153035737727	39.0	35.0	41.0	30.0	41.0
39	36.6298246641051	39.0	35.0	41.0	30.0	41.0
40	36.49678917472921	39.0	35.0	40.0	30.0	41.0
41	36.33326858464693	39.0	35.0	40.0	29.0	41.0
42	36.26490106755505	39.0	35.0	40.0	29.0	41.0
43	34.95741842552482	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	0.0
12	2.0
13	2.0
14	1.0
15	4.0
16	8.0
17	37.0
18	80.0
19	147.0
20	304.0
21	530.0
22	948.0
23	1544.0
24	2421.0
25	3745.0
26	5469.0
27	7896.0
28	10645.0
29	14552.0
30	19264.0
31	24846.0
32	32024.0
33	41573.0
34	56391.0
35	73803.0
36	100502.0
37	149005.0
38	261881.0
39	319806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48433081789121	17.12372143518568	11.267158401895983	28.124789345027125
2	20.37898381815021	19.010214327021664	32.2074157646199	28.403386090208226
3	20.759848009110936	20.93005722719516	28.266349486800134	30.04374527689377
4	15.500924220975737	14.362943883289162	31.920570214185105	38.21556168154999
5	16.242961906485156	35.26612597078681	32.25930341057053	16.231608712157495
6	37.94902770534203	32.21628544768838	14.695911785680075	15.138775061289511
7	32.17087267037774	28.47469834207884	19.49511990037572	19.8593090871677
8	29.65268095040428	31.167001940686657	18.460116583114253	20.720200525794812
9	28.149890548110935	13.072493693655337	17.31158132257618	41.46603443565755
10	19.031235475893975	24.7851762760813	30.16623560006954	26.01735264795518
11	38.99068328490486	19.993596088824553	19.430814698129208	21.584905928141378
12	24.811253144302647	22.914471420107215	26.395555933995368	25.878719501594773
13	31.456242305549935	16.99236142894142	23.32673428913038	28.22466197637826
14	24.13467371983864	18.62881795507683	22.725547170748495	34.51096115433604
15	27.60981554607091	25.11982941825523	19.705242692268122	27.565112343405744
16	27.422665233325883	23.563111342905497	20.76188803621669	28.25233538755193
17	26.072167289318415	24.50746649920705	22.887330189917655	26.533036021556878
18	26.305883438172987	21.99521746688947	24.104516797405793	27.594382297531745
19	26.993727360133967	22.585228784605068	23.03625216863751	27.384791686623455
20	26.587584572428057	23.108451388814974	22.994387264554263	27.309576774202704
21	29.535068952916177	22.48917011697338	21.954949105758555	26.02081182435189
22	28.366576905474012	22.88741888674834	22.27283854693304	26.473165660844604
23	27.04951766663474	22.863027258310005	22.353286572364194	27.734168502691066
24	27.301771453102436	23.082463217424316	22.694148492686057	26.921616836787187
25	27.221323427671283	22.488549239158587	23.12778729790427	27.16234003526586
26	28.061193717426093	23.2780397290844	23.130093415502078	25.53067313798743
27	28.747529793265425	22.28454652858344	22.05517652443243	26.9127471537187
28	26.537382166260436	22.11158770874799	24.007393767805887	27.343636357185687
29	26.428728548671497	22.41466477919811	24.2034137636194	26.953192908510992
30	25.937525500338822	23.21745979372665	23.96002966022018	26.884985045714348
31	26.69455295023398	23.287264199475626	22.926977673233782	27.091205177056615
32	25.93264717465116	22.832072064400997	23.284159810401654	27.951120950546194
33	26.90547401360255	22.78293402020159	23.18943159523024	27.122160370965627
34	27.608130306287897	23.1814488804686	22.940459591497877	26.269961221745625
35	28.00921737464477	22.66940207692499	23.917100394168717	25.404280154261528
36	26.824493807187284	23.763211392930508	22.142276812164948	27.270017987717267
37	26.271202977375214	23.079979706165137	23.43707314650233	27.21174416995732
38	27.00508055446163	22.59782373456232	24.017948690657384	26.379147020318673
39	25.918633075402948	22.748519649895872	23.956038302839364	27.376808971861816
40	26.554500654582608	21.989186082402902	25.127900829847547	26.328412433166935
41	24.924075512933772	22.26671846561579	25.81130991027429	26.997896111176157
42	25.79800538567156	22.720402754568774	24.84735275439138	26.634239105368284
43	25.316204201391475	20.39175616176883	26.25106879680975	28.040970840029942
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	35.5
2	39.0
3	84.5
4	130.0
5	130.0
6	209.5
7	289.0
8	262.0
9	235.0
10	341.0
11	447.0
12	447.0
13	892.5
14	1338.0
15	2166.5
16	2995.0
17	2709.5
18	2424.0
19	2424.0
20	2916.5
21	3409.0
22	3235.5
23	3062.0
24	3797.0
25	4532.0
26	4532.0
27	5856.0
28	7180.0
29	9187.0
30	11194.0
31	13805.5
32	16417.0
33	16417.0
34	20311.0
35	24205.0
36	29049.5
37	33894.0
38	38552.0
39	43210.0
40	43210.0
41	47239.5
42	51269.0
43	54131.0
44	56993.0
45	60582.5
46	64172.0
47	64172.0
48	67820.5
49	71469.0
50	75918.0
51	80367.0
52	87268.0
53	94169.0
54	94169.0
55	96058.5
56	97948.0
57	93855.5
58	89763.0
59	82712.0
60	75661.0
61	75661.0
62	73420.5
63	71180.0
64	62694.5
65	54209.0
66	46799.5
67	39390.0
68	39390.0
69	34211.5
70	29033.0
71	27047.0
72	25061.0
73	26606.5
74	28152.0
75	28152.0
76	26208.5
77	24265.0
78	18271.0
79	12277.0
80	7931.5
81	3586.0
82	3586.0
83	2505.0
84	1424.0
85	1131.0
86	838.0
87	738.0
88	638.0
89	638.0
90	544.5
91	451.0
92	258.5
93	66.0
94	40.5
95	15.0
96	15.0
97	9.5
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1127436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.68659232642789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08365766093344	35.85955288895269
2	9.923480994719117	8.670461373506983
3	2.855230129669415	3.742058240190047
4	1.325193922443702	2.315728265730316
5	0.7732949627144163	1.6891310892092484
6	0.505228523319285	1.3243027517959665
7	0.34019867091102257	1.0403484452257712
8	0.25567482445980777	0.8935649459445308
9	0.21618839652348146	0.8500080910162928
>10	1.348284852955124	11.65625722991204
>50	0.18270854693744376	5.651794392346113
>100	0.16251520945211895	14.957370397917638
>500	0.021002592885271038	6.223448357055217
>1k	0.007136803407616373	4.611888529571628
>5k	2.039086687890392E-4	0.5140850016255479
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5771	0.511869409882246	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3121	0.27682280856740427	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2341	0.20763928063322443	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2204	0.19548781482940053	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2156	0.1912303669565279	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1929	0.17109618639106786	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	1841	0.16329086529080142	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1836	0.1628473811373772	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1716	0.15220376145519568	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	1708	0.1514941868097169	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	1626	0.14422104669355953	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1599	0.14182623226506869	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1573	0.13952011466726272	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1553	0.1377461780535658	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1522	0.13499657630233558	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1482	0.13144870307494175	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1466	0.13002955378398418	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	1449	0.1285217076623418	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1387	0.12302250415988135	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1336	0.11849896579495421	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1292	0.11459630524482099	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1269	0.11255627813906953	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1234	0.10945188906509992	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	1199	0.1063474999911303	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	1181	0.10475095703880309	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1151	0.10209005211825771	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	1140	0.10111438698072439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.869683068484598E-5	0.0	0.0	0.0	0.0
3	8.869683068484598E-5	0.0	0.0	0.0	0.0
4	8.869683068484598E-5	0.0	0.0	0.0	0.0
5	1.7739366136969195E-4	0.0	0.0	0.0	0.0
6	1.7739366136969195E-4	0.0	0.0	0.0	0.0
7	2.660904920545379E-4	0.0	0.0	0.0	0.0
8	2.660904920545379E-4	0.0	0.0	0.0	0.0
9	2.660904920545379E-4	0.0	0.0	0.0	0.0
10	4.434841534242298E-4	0.0	0.0	0.0	0.0
11	7.095746454787678E-4	0.0	0.0	0.0	0.0
12	7.095746454787678E-4	0.0	0.0	0.0	8.869683068484598E-5
13	7.095746454787678E-4	0.0	0.0	8.869683068484598E-5	8.869683068484598E-5
14	7.095746454787678E-4	0.0	0.0	1.7739366136969195E-4	8.869683068484598E-5
15	7.095746454787678E-4	0.0	0.0	4.434841534242298E-4	8.869683068484598E-5
16	7.095746454787678E-4	0.0	0.0	6.208778147939218E-4	1.7739366136969195E-4
17	8.869683068484596E-4	0.0	0.0	7.982714761636138E-4	1.7739366136969195E-4
18	8.869683068484596E-4	0.0	0.0	9.756651375333056E-4	1.7739366136969195E-4
19	0.0010643619682181517	0.0	0.0	0.0013304524602726895	1.7739366136969195E-4
20	0.0010643619682181517	0.0	0.0	0.0015965429523272276	1.7739366136969195E-4
21	0.0012417556295878437	0.0	0.0	0.0018626334443817654	2.660904920545379E-4
22	0.0013304524602726895	0.0	0.0	0.002660904920545379	2.660904920545379E-4
23	0.0013304524602726895	0.0	0.0	0.004434841534242298	3.547873227393839E-4
24	0.0013304524602726895	0.0	0.0	0.00638617180930891	3.547873227393839E-4
25	0.0013304524602726895	0.0	0.0	0.00816010842300583	3.547873227393839E-4
26	0.0013304524602726895	0.0	0.0	0.01011143869807244	3.547873227393839E-4
27	0.0014191492909575356	0.0	0.0	0.01809415345970858	3.547873227393839E-4
28	0.0014191492909575356	0.0	0.0	0.0430179628821503	3.547873227393839E-4
29	0.0014191492909575356	0.0	0.0	0.07734363635718569	3.547873227393839E-4
30	0.0014191492909575356	0.0	0.0	0.1255947122497419	3.547873227393839E-4
31	0.0014191492909575356	0.0	0.0	0.24923809422441717	3.547873227393839E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGT	25	0.0054962994	29.6	6
TGCATCA	1310	0.0	21.324427	14
ATACACT	70	5.1017814E-6	21.142857	4
CAGGACA	1485	0.0	20.804714	4
CCGGTTA	45	0.0038257632	20.555555	27
GACAGGC	1405	0.0	20.409252	7
GCTGCAT	1405	0.0	20.145908	12
ACAGGCT	1415	0.0	20.134275	8
CCAGGAC	1465	0.0	20.078497	3
GCATCAG	1395	0.0	20.025091	15
CTGCATC	1460	0.0	19.386988	13
TCCAGGA	1595	0.0	18.905956	2
CGCAAGA	305	0.0	18.803278	2
TTTAGCG	50	0.0070348005	18.5	26
AAGACGG	300	0.0	18.5	5
ATCAGAA	1530	0.0	18.379086	17
AGAGGCC	1550	0.0	18.141937	23
TCTGTAC	115	6.40448E-8	17.695652	3
GTATAGA	115	6.40448E-8	17.695652	1
TTATTGT	115	6.40448E-8	17.695652	4
>>END_MODULE