FastQCFastQC Report
Fri 10 Feb 2017
ERR1630152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671950
Sequences flagged as poor quality0
Sequence length43
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT231643.447280303594017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT213603.1788079470198674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT148662.212366991591636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115401.7173896867326437No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA46840.6970756752734578No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.6500483666939504No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35070.5219138328744698No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.4924473547138924No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.48545278666567454No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32320.4809881687625567No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA30760.45777215566634427No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.3497284024108937No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.2622218915097849No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA16650.24778629362303745No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA16040.23870823722003126No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA15310.22784433365577797No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA15020.22352853634943076No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA14630.21772453307537765No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14320.21311109457548927No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13100.1949549817694769No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10640.158345114963911No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10290.15313639407694024No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA10190.15164818810923433No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9610.14301659349653992No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9550.14212366991591638No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9140.13602202544832204No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8990.13378971649676316No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA8940.1330456135129102No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8940.1330456135129102No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8430.12545576307760994No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT8340.12411637770667461No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA8280.12322345412605104No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7940.11816355383585089No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA7480.1113178063844036No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA7410.11027606220700946No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7280.10834139444899174No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA7060.10506734132003868No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA6730.10015626162660912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGC200.001841392437.04
GCTGTCC251.2330804E-436.9999968
TGTCCCC303.5979008E-430.83333410
CGACGAG250.005494740329.59999824
TAGCACA250.005494740329.5999984
ACGCTGC250.005494740329.59999831
TACTAAA250.005494740329.5999982
CAATGCG250.005494740329.59999819
CTGTCCC405.9365157E-527.759
AGACTAT509.083755E-625.8999986
GTACTGG509.083755E-625.8999981
ATGGTTG451.3224136E-424.66666618
TGTTATG400.001930357423.1255
AATGTGG400.001930357423.12513
CTCAGTA400.001930357423.12530
GTCAGTC502.7004848E-422.19999918
ATCCGGA502.7004848E-422.1999991
ACCAGTT502.7004848E-422.1999997
CGGTCAG502.7004848E-422.19999916
ATACCAG1005.329639E-1022.1999995
Produced by FastQC (version 0.11.5)