##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	671950
Sequences flagged as poor quality	0
Sequence length	43
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76559118982067	31.0	31.0	34.0	30.0	34.0
2	31.82474886524295	33.0	31.0	34.0	30.0	34.0
3	31.852347644914055	33.0	31.0	34.0	30.0	34.0
4	35.58273681077461	37.0	35.0	37.0	33.0	37.0
5	35.55783317211102	37.0	35.0	37.0	33.0	37.0
6	35.71557705186398	37.0	35.0	37.0	33.0	37.0
7	35.69237443262148	37.0	35.0	37.0	33.0	37.0
8	35.78663888682193	37.0	35.0	37.0	35.0	37.0
9	37.39485378376367	39.0	37.0	39.0	34.0	39.0
10	37.32081404866433	39.0	37.0	39.0	34.0	39.0
11	37.44206265347124	39.0	37.0	39.0	35.0	39.0
12	37.370238857057814	39.0	37.0	39.0	34.0	39.0
13	37.448220849765605	39.0	37.0	39.0	35.0	39.0
14	38.46964655108267	40.0	38.0	41.0	34.0	41.0
15	38.4321288786368	40.0	38.0	41.0	34.0	41.0
16	38.307735694620135	40.0	38.0	41.0	34.0	41.0
17	38.50697373316467	40.0	38.0	41.0	34.0	41.0
18	38.61710097477491	40.0	38.0	41.0	35.0	41.0
19	38.689892105067344	40.0	38.0	41.0	34.0	41.0
20	38.67605774239155	40.0	38.0	41.0	35.0	41.0
21	38.6613319443411	40.0	38.0	41.0	35.0	41.0
22	38.587826475184166	40.0	38.0	41.0	34.0	41.0
23	38.476850956172335	40.0	38.0	41.0	34.0	41.0
24	38.41239675571099	40.0	38.0	41.0	34.0	41.0
25	38.35646699903267	40.0	37.0	41.0	34.0	41.0
26	38.04552719696406	40.0	37.0	41.0	34.0	41.0
27	37.794952005357544	40.0	36.0	41.0	33.0	41.0
28	37.56724756306273	39.0	35.0	41.0	33.0	41.0
29	37.31890468040777	39.0	35.0	41.0	33.0	41.0
30	36.996539921125084	39.0	35.0	40.0	33.0	41.0
31	36.58267877074187	38.0	35.0	40.0	32.0	41.0
32	36.219284172929534	38.0	35.0	40.0	31.0	41.0
33	35.9189909963539	38.0	35.0	40.0	31.0	41.0
34	35.667527345784656	38.0	35.0	40.0	30.0	41.0
35	35.365885854602276	37.0	35.0	40.0	30.0	41.0
36	34.99978272192872	37.0	35.0	40.0	25.0	41.0
37	34.64006994568048	37.0	35.0	40.0	24.0	41.0
38	34.24731155591934	36.0	34.0	40.0	21.0	41.0
39	33.79272713743582	36.0	33.0	40.0	18.0	41.0
40	33.29686583823201	35.0	33.0	40.0	15.0	41.0
41	32.71981843887194	35.0	33.0	40.0	10.0	41.0
42	32.20303445196815	35.0	33.0	40.0	8.0	41.0
43	31.212005357541482	35.0	30.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	9.0
11	8.0
12	6.0
13	4.0
14	1.0
15	4.0
16	10.0
17	13.0
18	36.0
19	95.0
20	196.0
21	385.0
22	723.0
23	1164.0
24	2173.0
25	3483.0
26	5330.0
27	7538.0
28	10384.0
29	13663.0
30	16520.0
31	20079.0
32	24754.0
33	31262.0
34	39740.0
35	50188.0
36	70820.0
37	119202.0
38	131627.0
39	122527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.98906168613736	20.153880497060793	14.698563881241164	16.15849393556068
2	17.123149043827667	28.818810923431805	32.884589627204406	21.173450405536126
3	21.581814123074633	31.559044571768734	30.672073815016	16.187067490140635
4	14.197484931914579	18.875213929607856	32.753032219659204	34.17426891881836
5	10.9235806235583	43.6321154847831	31.30590073666195	14.13840315499665
6	27.060197931393702	34.08929235806236	19.26244512240494	19.588064588138998
7	22.768956023513653	36.07887491628842	24.812113996577125	16.340055063620806
8	29.969938239452343	31.959074335888086	21.32747972319369	16.74350770146588
9	27.998214152838752	13.01614703474961	24.55152913163182	34.43410968077982
10	20.221891509784953	31.654289753701914	28.5065853114071	19.617233425106033
11	25.691048441104247	29.087283280005956	20.660465808467894	24.561202470421904
12	18.14495126125456	36.109085497432844	29.2206265347124	16.525336706600193
13	28.76508668799762	22.256715529429272	29.9645806979686	19.01361708460451
14	21.81948061611727	25.71991963687774	32.65540590817769	19.805193838827293
15	24.538135277922464	28.650792469677803	29.413200386933553	17.39787186546618
16	16.591115410372794	28.585757868889054	34.27948508073517	20.543641640002978
17	15.955056179775282	33.129697150085576	32.87134459409182	18.043902076047324
18	14.320708386040629	27.537465585236998	36.91227025820373	21.22955577051864
19	15.670362378153136	30.252846193913236	40.347496093459334	13.729295334474292
20	16.12828335441625	28.82476374730263	40.68695587469306	14.359997023588065
21	18.277252771783616	28.534861224793513	38.635166307016895	14.552719696405983
22	18.054319517821266	29.185951335664857	37.56395565146216	15.195773495051714
23	16.152094649899546	30.595580028275915	38.01398913609644	15.238336185728105
24	14.126051045464694	32.82654959446387	37.77364387231193	15.273755487759505
25	14.702284396160428	31.76233350695736	38.20700945010789	15.328372646774312
26	15.459781233722747	31.45055435672297	38.145248902448095	14.944415507106184
27	14.170846045092642	32.87595803259171	37.38313862638589	15.570057295929757
28	14.42190639184463	32.753925143239826	39.70146588287819	13.122702582037354
29	14.999776769104844	32.586353151276136	38.88890542451075	13.524964655108269
30	13.858471612471165	34.43485378376367	38.29406949921869	13.412605104546468
31	14.359699382394522	34.079916660465805	38.06458813899844	13.495795818141232
32	12.72029168836967	34.86985638812411	38.29779001413796	14.112061909368256
33	12.51447280303594	35.04873874544237	38.39705335218394	14.039735099337749
34	13.477639705335218	34.51432398243917	37.02462980876554	14.983406503460078
35	12.99352630404048	35.134905870972545	36.89143537465585	14.980132450331126
36	12.99575861299204	35.01882580549148	35.79611578242429	16.189299799092193
37	12.897090557333135	34.47697001264975	36.97358434407322	15.652355085943896
38	13.053054542748715	34.42160875065109	36.77446238559416	15.750874321006028
39	12.653471240419675	34.294664781605775	37.68494679663665	15.366917181337897
40	12.995312151201727	34.09688220849766	37.592231564848575	15.315574075452043
41	12.068755115708015	34.61834957958182	37.157973063471985	16.154922241238186
42	11.745367958925515	34.74559118982067	36.62921348314607	16.879827368107748
43	11.746112061909368	34.527866656745296	36.83384180370563	16.892179477639704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	505.0
2	754.0
3	2607.0
4	4460.0
5	4460.0
6	6206.5
7	7953.0
8	7801.0
9	7649.0
10	10311.0
11	12973.0
12	12973.0
13	23426.0
14	33879.0
15	46466.5
16	59054.0
17	48485.0
18	37916.0
19	37916.0
20	40640.5
21	43365.0
22	29679.0
23	15993.0
24	15574.5
25	15156.0
26	15156.0
27	15119.5
28	15083.0
29	15800.5
30	16518.0
31	17623.5
32	18729.0
33	18729.0
34	21438.0
35	24147.0
36	25340.0
37	26533.0
38	28766.5
39	31000.0
40	31000.0
41	35587.0
42	40174.0
43	42903.5
44	45633.0
45	49752.5
46	53872.0
47	53872.0
48	54380.5
49	54889.0
50	46069.0
51	37249.0
52	30620.5
53	23992.0
54	23992.0
55	20522.5
56	17053.0
57	13637.0
58	10221.0
59	8735.5
60	7250.0
61	7250.0
62	6014.0
63	4778.0
64	3627.0
65	2476.0
66	1948.5
67	1421.0
68	1421.0
69	990.5
70	560.0
71	459.5
72	359.0
73	302.5
74	246.0
75	246.0
76	191.0
77	136.0
78	108.5
79	81.0
80	71.5
81	62.0
82	62.0
83	52.0
84	42.0
85	30.5
86	19.0
87	17.0
88	15.0
89	15.0
90	9.0
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	671950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.617570788031937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.47982690587672	16.64562301077781
2	10.83733350686656	5.119029825038628
3	4.600029775565872	3.259245865544449
4	2.699663825692713	2.55038006028747
5	1.822707455876198	2.152396118251485
6	1.3233201870166897	1.8752164915258995
7	0.9854942108678969	1.6292485500457758
8	0.7685749708684866	1.452149902431685
9	0.6315616268234219	1.3424356285656094
>10	4.9890316454976835	23.875602886193718
>50	0.5694986910110288	9.278235357094879
>100	0.26012578791191115	10.7406064500689
>500	0.01830982487729472	3.065954118755871
>1k	0.011996092160986195	6.437146702620115
>5k	0.0	0.0
>10k+	0.0025254930865234097	10.57672903279772
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23164	3.447280303594017	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21360	3.1788079470198674	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14866	2.212366991591636	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11540	1.7173896867326437	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4684	0.6970756752734578	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4368	0.6500483666939504	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3507	0.5219138328744698	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3309	0.4924473547138924	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3262	0.48545278666567454	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3232	0.4809881687625567	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	3076	0.45777215566634427	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2350	0.3497284024108937	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1762	0.2622218915097849	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	1665	0.24778629362303745	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1604	0.23870823722003126	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1531	0.22784433365577797	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1502	0.22352853634943076	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1463	0.21772453307537765	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.21311109457548927	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1310	0.1949549817694769	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1064	0.158345114963911	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1029	0.15313639407694024	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	1019	0.15164818810923433	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	961	0.14301659349653992	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	955	0.14212366991591638	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	914	0.13602202544832204	No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	899	0.13378971649676316	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	894	0.1330456135129102	No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	894	0.1330456135129102	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	843	0.12545576307760994	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	834	0.12411637770667461	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	828	0.12322345412605104	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	794	0.11816355383585089	No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	748	0.1113178063844036	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	741	0.11027606220700946	No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	728	0.10834139444899174	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	706	0.10506734132003868	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	673	0.10015626162660912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4882059677059304E-4	0.0
2	0.0	0.0	0.0	1.4882059677059304E-4	0.0
3	0.0	0.0	0.0	1.4882059677059304E-4	0.0
4	0.0	0.0	0.0	1.4882059677059304E-4	0.0
5	0.0	0.0	0.0	1.4882059677059304E-4	0.0
6	0.0	0.0	0.0	1.4882059677059304E-4	0.0
7	0.0	0.0	0.0	1.4882059677059304E-4	0.0
8	0.0	0.0	0.0	2.976411935411861E-4	0.0
9	0.0	0.0	0.0	5.952823870823722E-4	0.0
10	0.0	0.0	0.0	0.0013393853709353374	0.0
11	0.0	0.0	0.0	0.003125232532182454	0.0
12	0.0	0.0	0.0	0.006101644467594315	0.0
13	0.0	0.0	0.0	0.007887491628841432	0.0
14	0.0	0.0	0.0	0.010566262370712107	0.0
15	0.0	0.0	0.0	0.01413795669320634	0.0
16	0.0	0.0	0.0	0.026043604434853783	0.0
17	0.0	0.0	0.0	0.04018156112806012	0.0
18	0.0	0.0	0.0	0.045539102611801476	0.0
19	0.0	0.0	0.0	0.05655182677282536	0.0
20	0.0	0.0	0.0	0.06771337153061983	0.0
21	0.0	0.0	0.0	0.08899471686881465	0.0
22	0.0	0.0	0.0	0.12887863680333358	0.0
23	0.0	0.0	0.0	0.17516184239898802	0.0
24	0.0	0.0	0.0	0.23662474886524296	0.0
25	0.0	0.0	0.0	0.2583525559937495	0.0
26	0.0	0.0	0.0	0.2952600639928566	0.0
27	0.0	0.0	0.0	0.33990624302403455	0.0
28	0.0	0.0	0.0	0.4290497804896198	0.0
29	0.0	0.0	0.0	0.5635835999702359	0.0
30	0.0	0.0	0.0	0.8354788302701094	0.0
31	0.0	0.0	0.0	1.4173673636431283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGAGC	20	0.0018413924	37.0	4
GCTGTCC	25	1.2330804E-4	36.999996	8
TGTCCCC	30	3.5979008E-4	30.833334	10
CGACGAG	25	0.0054947403	29.599998	24
TAGCACA	25	0.0054947403	29.599998	4
ACGCTGC	25	0.0054947403	29.599998	31
TACTAAA	25	0.0054947403	29.599998	2
CAATGCG	25	0.0054947403	29.599998	19
CTGTCCC	40	5.9365157E-5	27.75	9
AGACTAT	50	9.083755E-6	25.899998	6
GTACTGG	50	9.083755E-6	25.899998	1
ATGGTTG	45	1.3224136E-4	24.666666	18
TGTTATG	40	0.0019303574	23.125	5
AATGTGG	40	0.0019303574	23.125	13
CTCAGTA	40	0.0019303574	23.125	30
GTCAGTC	50	2.7004848E-4	22.199999	18
ATCCGGA	50	2.7004848E-4	22.199999	1
ACCAGTT	50	2.7004848E-4	22.199999	7
CGGTCAG	50	2.7004848E-4	22.199999	16
ATACCAG	100	5.329639E-10	22.199999	5
>>END_MODULE