FastQCFastQC Report
Fri 10 Feb 2017
ERR1630151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432660
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG19030.1328298409950721No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG14410.10058213393268467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG308.31342E-637.036
GCGATCG358.8704657E-426.42857235
TAACGGC555.143946E-420.18181836
AAGACCG759.268388E-619.7333345
CTTATAC4900.019.63265237
GGTATCA9750.018.7846151
TATACTG1603.45608E-1117.343755
GCGTTAT1301.3938916E-817.0769231
TCTTATA9100.016.46703137
ATAAGAC803.383294E-416.18753
TCGCCAT3550.016.1549313
ATTAGAC700.002592911515.8571433
AGTCGTG700.002592911515.8571437
GTTAGCC1101.4522864E-515.1363643
CGTACTC750.004105062714.83
GTCGCCA3800.014.60526412
TCCTATA908.275767E-414.3888892
TCCTAAG1803.3305696E-914.3888892
ATACCGC1304.4455137E-614.23076927
CGATTTG1458.913594E-714.03448328
Produced by FastQC (version 0.11.5)