##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630151.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1432660
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87256222690659	33.0	31.0	34.0	30.0	34.0
2	32.072677397288956	33.0	31.0	34.0	30.0	34.0
3	32.214696438792174	34.0	31.0	34.0	30.0	34.0
4	35.860658495386204	37.0	35.0	37.0	35.0	37.0
5	35.77277372160876	37.0	35.0	37.0	35.0	37.0
6	35.8408687336842	37.0	35.0	37.0	35.0	37.0
7	35.817164574986386	37.0	35.0	37.0	35.0	37.0
8	35.794628872167856	37.0	35.0	37.0	35.0	37.0
9	37.51959152904388	39.0	37.0	39.0	35.0	39.0
10	37.43806974439155	39.0	37.0	39.0	34.0	39.0
11	37.5146371085952	39.0	37.0	39.0	35.0	39.0
12	37.399023494758005	39.0	37.0	39.0	34.0	39.0
13	37.4210636159312	39.0	37.0	39.0	34.0	39.0
14	38.66462733656276	40.0	38.0	41.0	34.0	41.0
15	38.6665621989865	40.0	38.0	41.0	35.0	41.0
16	38.64545111889772	40.0	38.0	41.0	34.0	41.0
17	38.611475856099844	40.0	38.0	41.0	34.0	41.0
18	38.57974676475926	40.0	38.0	41.0	34.0	41.0
19	38.6072543380844	40.0	38.0	41.0	34.0	41.0
20	38.55210308098223	40.0	38.0	41.0	34.0	41.0
21	38.514307651501404	40.0	38.0	41.0	34.0	41.0
22	38.49607583097176	40.0	38.0	41.0	34.0	41.0
23	38.44575474990577	40.0	38.0	41.0	34.0	41.0
24	38.43760068683428	40.0	38.0	41.0	34.0	41.0
25	38.40778342384097	40.0	38.0	41.0	34.0	41.0
26	38.261265059399996	40.0	38.0	41.0	34.0	41.0
27	38.17823558974216	40.0	38.0	41.0	34.0	41.0
28	38.11739700975807	40.0	38.0	41.0	33.0	41.0
29	38.063496572808624	40.0	38.0	41.0	33.0	41.0
30	38.025809333686986	40.0	38.0	41.0	33.0	41.0
31	37.93046291513688	40.0	37.0	41.0	33.0	41.0
32	37.842480421035	40.0	37.0	41.0	33.0	41.0
33	37.80015565451677	40.0	37.0	41.0	33.0	41.0
34	37.723049432524114	40.0	37.0	41.0	33.0	41.0
35	37.641946449262214	40.0	37.0	41.0	32.0	41.0
36	37.56008822749291	40.0	37.0	41.0	32.0	41.0
37	37.512844638644204	40.0	37.0	41.0	32.0	41.0
38	37.42175812823699	40.0	37.0	41.0	32.0	41.0
39	37.34081428950344	39.0	37.0	41.0	31.0	41.0
40	37.274698812000054	39.0	36.0	41.0	31.0	41.0
41	37.172521044769866	39.0	36.0	41.0	31.0	41.0
42	37.139294040456214	39.0	36.0	41.0	31.0	41.0
43	35.94844903885081	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	1.0
12	1.0
13	0.0
14	1.0
15	3.0
16	5.0
17	27.0
18	55.0
19	103.0
20	190.0
21	335.0
22	666.0
23	1160.0
24	2066.0
25	3119.0
26	4807.0
27	7149.0
28	10366.0
29	14491.0
30	20160.0
31	27046.0
32	35776.0
33	47366.0
34	63014.0
35	85579.0
36	120262.0
37	180726.0
38	338142.0
39	470042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22495218684126	18.507810645931343	12.388912931190932	26.878324236036466
2	19.140061145003003	20.635461309731546	33.873982661622435	26.350494883643016
3	20.11642678653693	21.990563008669188	29.486340094649115	28.406670110144766
4	15.584716541258917	15.244859212932587	33.17186213058228	35.99856211522622
5	15.169614563120348	35.86461547052336	34.06579369843507	14.899976267921208
6	36.23602250359471	33.690198651459525	15.0142392472743	15.059539597671465
7	30.18189940390602	29.70320941465526	20.534948976030602	19.57994220540812
8	27.297893429006187	33.523794898999064	19.65707146147725	19.521240210517497
9	26.935839626987562	14.120935881507126	18.59994695182388	40.34327753968143
10	17.22585958985384	26.674507559365097	32.791311267153404	23.30832158362766
11	36.363756927673	21.296469504278754	20.3825052699175	21.95726829813075
12	21.77411249005347	24.960702469532198	28.505507238284032	24.7596778021303
13	30.88925495232644	19.185152094704954	24.28098781287954	25.644605140089066
14	23.67965881646727	20.276757918836292	23.618025211843705	32.425558052852736
15	26.08329959655466	26.980651375762566	21.3183867770441	25.617662250638674
16	25.903284798905535	25.7847640054165	22.80897072578281	25.50298046989516
17	24.531849845741487	25.946072340960175	24.38122094565354	25.1408568676448
18	24.172448452528865	24.878756997473232	25.600281992936218	25.348512557061692
19	25.402119135035527	24.80302374603884	25.389415492859435	24.4054416260662
20	25.748747085840325	24.30276548518141	25.28625075035249	24.662236678625774
21	25.40875015705052	24.794019516144793	24.21488699342482	25.582343333379864
22	25.644186338698642	24.528708835313335	24.4136082531794	25.41349657280862
23	24.92182374045482	25.014448647969513	24.669565702958135	25.394161908617534
24	25.560356260382783	25.106654754093782	24.608769701115406	24.724219284408026
25	25.88325213239708	24.54713609649184	24.53673586196306	25.032875909148018
26	25.47792218670166	25.00670082224673	24.365725294207977	25.149651696843634
27	25.594279173006857	24.45137017854899	24.22563622911228	25.728714419331872
28	25.07503524911703	24.65476805382994	24.894392249382268	25.37580444767077
29	24.74034313793922	24.934108581240487	24.97878072955202	25.346767551268268
30	24.770636438512973	25.80486647215669	24.631175575502912	24.793321513827426
31	25.555260843465998	24.993857579607166	24.52277581561571	24.92810576131113
32	24.57156617759971	25.020381667667134	24.87296357823908	25.535088576494076
33	24.75695559309257	24.80853796434604	24.99434618122932	25.440160261332068
34	25.409238758672682	24.272541984839393	25.01703125654377	25.30118799994416
35	25.14092666787654	24.585107422556643	25.28562254826686	24.988343361299957
36	24.76896123295129	24.9369703907417	25.10093113509137	25.19313724121564
37	25.58597294543018	23.82233049013723	25.042019739505534	25.549676824927058
38	24.76561082182793	24.265561961665714	25.90377340052769	25.065053815978672
39	24.800022336074157	23.87928747923443	26.11275529434758	25.207934890343836
40	24.15367219019167	23.46579090642581	26.969692739379898	25.410844164002626
41	23.678472212527744	23.72265575921712	27.25713009367191	25.341741934583222
42	24.12191308475144	23.3030865662474	26.959641506009802	25.61535884299136
43	23.561626624600393	23.105761311127555	27.345217986123714	25.98739407814834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	37.0
2	49.0
3	116.0
4	183.0
5	183.0
6	287.0
7	391.0
8	352.5
9	314.0
10	468.5
11	623.0
12	623.0
13	1092.5
14	1562.0
15	2592.5
16	3623.0
17	3602.0
18	3581.0
19	3581.0
20	4646.0
21	5711.0
22	6690.5
23	7670.0
24	9567.5
25	11465.0
26	11465.0
27	13814.0
28	16163.0
29	21729.0
30	27295.0
31	31118.5
32	34942.0
33	34942.0
34	39486.5
35	44031.0
36	49204.5
37	54378.0
38	61934.0
39	69490.0
40	69490.0
41	76012.5
42	82535.0
43	82247.5
44	81960.0
45	88784.5
46	95609.0
47	95609.0
48	100717.0
49	105825.0
50	111781.0
51	117737.0
52	124686.0
53	131635.0
54	131635.0
55	121742.0
56	111849.0
57	105737.0
58	99625.0
59	91125.0
60	82625.0
61	82625.0
62	77236.0
63	71847.0
64	63621.0
65	55395.0
66	47747.0
67	40099.0
68	40099.0
69	33778.0
70	27457.0
71	23728.0
72	19999.0
73	16142.5
74	12286.0
75	12286.0
76	9436.0
77	6586.0
78	5425.5
79	4265.0
80	3155.0
81	2045.0
82	2045.0
83	1472.0
84	899.0
85	678.0
86	457.0
87	343.5
88	230.0
89	230.0
90	195.0
91	160.0
92	93.5
93	27.0
94	17.5
95	8.0
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1432660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.40931270956978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.42374579557234	49.31132968286812
2	9.300743688203726	10.865000930316977
3	2.494228853292327	4.370585791835495
4	1.064850287073741	2.4878869362626115
5	0.583588589499616	1.7043504208909908
6	0.38902048330649036	1.363345143592606
7	0.269412662389351	1.1015345911792147
8	0.19304300909233862	0.9020407587576582
9	0.15427402175344318	0.810993562160385
>10	0.9331911149498532	10.346374180718056
>50	0.10287519948258216	4.231943101875987
>100	0.0836005103967013	9.34142753748981
>500	0.006587389908290309	2.513704181998656
>1k	8.383950792369486E-4	0.6494831800533096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1903	0.1328298409950721	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1441	0.10058213393268467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.980023173676937E-5	0.0	0.0	0.0	0.0
6	6.980023173676937E-5	0.0	0.0	0.0	0.0
7	6.980023173676937E-5	0.0	0.0	0.0	0.0
8	6.980023173676937E-5	0.0	0.0	0.0	0.0
9	6.980023173676937E-5	0.0	0.0	0.0	0.0
10	6.980023173676937E-5	0.0	0.0	6.980023173676937E-5	0.0
11	6.980023173676937E-5	0.0	0.0	6.980023173676937E-5	0.0
12	6.980023173676937E-5	0.0	0.0	6.980023173676937E-5	0.0
13	6.980023173676937E-5	0.0	0.0	6.980023173676937E-5	0.0
14	6.980023173676937E-5	0.0	0.0	6.980023173676937E-5	0.0
15	6.980023173676937E-5	0.0	0.0	6.980023173676937E-5	0.0
16	6.980023173676937E-5	0.0	0.0	3.490011586838468E-4	0.0
17	6.980023173676937E-5	0.0	0.0	6.980023173676936E-4	0.0
18	1.3960046347353874E-4	0.0	0.0	7.67802549104463E-4	0.0
19	1.3960046347353874E-4	0.0	0.0	0.00111680370778831	0.0
20	1.3960046347353874E-4	0.0	0.0	0.0011866039395250793	0.0
21	1.3960046347353874E-4	0.0	0.0	0.0016054053299456954	0.0
22	2.094006952103081E-4	0.0	0.0	0.0025128083425236974	0.0
23	2.094006952103081E-4	0.0	0.0	0.004048413440732623	0.0
24	2.094006952103081E-4	0.0	0.0	0.006282020856309243	0.0
25	2.094006952103081E-4	0.0	0.0	0.007538425027571092	0.0
26	2.094006952103081E-4	0.0	0.0	0.00991163290662125	0.0
27	2.094006952103081E-4	0.0	0.0	0.017101056775508494	0.0
28	2.094006952103081E-4	0.0	0.0	0.03790152583306577	0.0
29	2.094006952103081E-4	0.0	0.0	0.06784582524813983	0.0
30	2.094006952103081E-4	0.0	0.0	0.11021456591235883	0.0
31	2.094006952103081E-4	0.0	0.0	0.25735345441346863	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGG	30	8.31342E-6	37.0	36
GCGATCG	35	8.8704657E-4	26.428572	35
TAACGGC	55	5.143946E-4	20.181818	36
AAGACCG	75	9.268388E-6	19.733334	5
CTTATAC	490	0.0	19.632652	37
GGTATCA	975	0.0	18.784615	1
TATACTG	160	3.45608E-11	17.34375	5
GCGTTAT	130	1.3938916E-8	17.076923	1
TCTTATA	910	0.0	16.467031	37
ATAAGAC	80	3.383294E-4	16.1875	3
TCGCCAT	355	0.0	16.15493	13
ATTAGAC	70	0.0025929115	15.857143	3
AGTCGTG	70	0.0025929115	15.857143	7
GTTAGCC	110	1.4522864E-5	15.136364	3
CGTACTC	75	0.0041050627	14.8	3
GTCGCCA	380	0.0	14.605264	12
TCCTATA	90	8.275767E-4	14.388889	2
TCCTAAG	180	3.3305696E-9	14.388889	2
ATACCGC	130	4.4455137E-6	14.230769	27
CGATTTG	145	8.913594E-7	14.034483	28
>>END_MODULE