FastQCFastQC Report
Fri 10 Feb 2017
ERR1630150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115214
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19451.6881628968701718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16601.44079712534935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12671.0996927456732688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.601489402329578No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3810.3306889787699412No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2410.20917596819830922No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.1779297654798896No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.16664641449823805No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.16317461419619145No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.15102331313902825No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1610.1397399621573767No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1600.13887201208186503No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1510.13106046140226013No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.11717326019407363No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1300.11283350981651535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAAT250.005476092529.5999981
GCGTGAT250.005476092529.59999836
AGGGCGT250.005476092529.59999833
TACCATG400.001920715623.1257
AGATGTG400.001920715623.1255
ATACCAT603.6889636E-521.5833326
TTTTGCG450.00380526120.5555552
TATACTC450.00380526120.5555555
AGCCTCC609.16823E-418.519
TTGCGTT500.00699746318.4999984
CTACCAC500.00699746318.4999983
CGGTGAT1351.1150405E-917.81481614
CAGTCGG1351.1150405E-917.81481610
TCGGTGA1351.1150405E-917.81481613
TCCTCGC1054.7053618E-717.61904721
TTCCTCG1054.7053618E-717.61904720
GCCTTAT1107.6588185E-716.81818226
CGCCTTA1107.6588185E-716.81818225
CTCGCCT1107.6588185E-716.81818223
CTATACT904.3903623E-516.4444454
Produced by FastQC (version 0.11.5)