##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	115214
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.758041557449616	31.0	31.0	34.0	30.0	34.0
2	31.918117589876232	33.0	31.0	34.0	30.0	34.0
3	31.961402260142	33.0	31.0	34.0	30.0	34.0
4	35.69017654104536	37.0	35.0	37.0	33.0	37.0
5	35.64166681132501	37.0	35.0	37.0	33.0	37.0
6	35.753285191035815	37.0	35.0	37.0	35.0	37.0
7	35.7381655007204	37.0	35.0	37.0	33.0	37.0
8	35.76069748468068	37.0	35.0	37.0	35.0	37.0
9	37.413821237002445	39.0	37.0	39.0	34.0	39.0
10	37.31412849132918	39.0	37.0	39.0	34.0	39.0
11	37.411200027774406	39.0	37.0	39.0	34.0	39.0
12	37.296526463797804	39.0	37.0	39.0	34.0	39.0
13	37.36292464457444	39.0	37.0	39.0	34.0	39.0
14	38.45868557640564	40.0	38.0	41.0	34.0	41.0
15	38.41672019025466	40.0	38.0	41.0	34.0	41.0
16	38.33032443973823	40.0	38.0	41.0	34.0	41.0
17	38.39544673390387	40.0	38.0	41.0	34.0	41.0
18	38.45545680212474	40.0	38.0	41.0	34.0	41.0
19	38.512819622615304	40.0	38.0	41.0	34.0	41.0
20	38.497847483812734	40.0	38.0	41.0	34.0	41.0
21	38.51308868713872	40.0	38.0	41.0	34.0	41.0
22	38.469118336313294	40.0	38.0	41.0	34.0	41.0
23	38.427187668165324	40.0	38.0	41.0	34.0	41.0
24	38.39194889509955	40.0	38.0	41.0	34.0	41.0
25	38.36280313156387	40.0	38.0	41.0	34.0	41.0
26	38.15225580224625	40.0	37.0	41.0	34.0	41.0
27	38.02525734719739	40.0	37.0	41.0	33.0	41.0
28	37.919072334959296	40.0	37.0	41.0	33.0	41.0
29	37.790285902754874	40.0	37.0	41.0	33.0	41.0
30	37.63662402138629	39.0	37.0	41.0	33.0	41.0
31	37.41024528269134	39.0	36.0	41.0	33.0	41.0
32	37.23196833718124	39.0	35.0	41.0	32.0	41.0
33	37.1056034856875	39.0	35.0	41.0	32.0	41.0
34	37.00740361414412	39.0	35.0	41.0	31.0	41.0
35	36.88576909056191	39.0	35.0	41.0	31.0	41.0
36	36.75435276962869	39.0	35.0	41.0	31.0	41.0
37	36.61599284809138	39.0	35.0	41.0	31.0	41.0
38	36.474768691304874	39.0	35.0	41.0	30.0	41.0
39	36.27267519572274	39.0	35.0	40.0	30.0	41.0
40	36.0600447862239	39.0	35.0	40.0	30.0	41.0
41	35.824517853733056	38.0	35.0	40.0	28.0	41.0
42	35.652767892790806	38.0	35.0	40.0	27.0	41.0
43	34.50457409689795	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	6.0
19	8.0
20	11.0
21	36.0
22	59.0
23	110.0
24	215.0
25	343.0
26	560.0
27	837.0
28	1174.0
29	1530.0
30	2005.0
31	2598.0
32	3372.0
33	4352.0
34	5877.0
35	7599.0
36	10858.0
37	17739.0
38	26280.0
39	29640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49632857118059	19.06105160831149	14.311628795111705	22.13099102539622
2	19.46551634349992	22.699498324856354	34.06617251375701	23.768812817886715
3	19.461176593122364	26.445570850764668	31.413717082993386	22.679535473119586
4	13.719686843612756	17.883243355842172	34.97665214296873	33.42041765757634
5	12.44380023261062	39.85626746749527	34.073984064436615	13.625948235457496
6	30.192511326748484	35.48093113684101	17.29477320464527	17.031784331765238
7	26.91599979169198	33.07150172722065	22.027704966410333	17.984793514677037
8	29.35146770357769	32.45438922353186	19.032409255819605	19.161733817070843
9	27.70757026055861	13.280504105403857	20.45845122988526	38.55347440415227
10	19.723297515926884	28.3021160622841	29.65611818008228	22.31846824170674
11	31.883278073845194	25.513392469665146	18.959501449476626	23.643828007013038
12	20.682382349367266	30.638637665561475	27.36039023035395	21.31858975471731
13	31.531758293262975	20.330862568785044	25.561129723818286	22.5762494141337
14	23.642960056937525	22.674327772666516	26.3440206919298	27.33869147846616
15	27.6468137552728	27.104344958078013	23.365216032773795	21.883625253875397
16	22.594476365719444	26.110542121617165	27.634662454215636	23.660319058447758
17	20.771781207144965	29.27682399708369	26.94637804433489	23.005016751436457
18	20.19719825715625	25.372784557432254	29.57192702275765	24.858090162653845
19	21.29862690298054	27.55307514711754	31.57168399673651	19.576613953165413
20	22.348846494349644	25.298140850938257	32.03169753675768	20.321315117954416
21	23.69156526116618	25.42486156196296	29.994618709531828	20.888954467339037
22	23.785303869321435	25.249535646709603	29.091082680924192	21.87407780304477
23	22.24295658513722	26.676445570850767	29.480792264828924	21.599805579183084
24	20.232784210252227	28.06169389136737	29.789782491711076	21.915739406669328
25	21.657090284166856	26.93683059350426	29.883521099866332	21.522558022462547
26	22.097140972451264	26.711163573871232	29.593625774645442	21.59806967903206
27	20.835141562657316	27.64854965542382	28.900133664311628	22.616175117607234
28	20.896766018018646	27.43850573715	31.29480792264829	20.36992032218307
29	21.587654278125925	27.289218324161997	31.12382175777249	19.999305639939593
30	20.62856944468554	28.88364261287691	30.361761591473257	20.126026350964292
31	21.441838665439963	28.07471314250004	29.972919957644034	20.510528234415958
32	19.9776068880518	28.76299755238079	30.372176992379394	20.887218567188015
33	19.833527175516863	28.799451455552276	30.603919662541013	20.763101706389847
34	21.101602235839394	28.0564861909143	29.48513201520648	21.356779558039822
35	20.072213446282568	29.55456802124742	29.63355147811898	20.73966705435103
36	19.781450170986165	29.22127519225094	28.80031940562779	22.196955231135103
37	20.11040324960508	28.756053951776693	29.949485305605222	21.184057493013004
38	19.905567031784333	29.00341972329751	29.959900706511363	21.13111253840679
39	19.793601472043328	28.269133959414656	30.816567431041364	21.120697137500652
40	19.603520405506274	28.2057736039023	31.02313954901314	21.16756644157828
41	18.881385942680577	28.77428090336244	30.704601871300362	21.639731282656623
42	18.314614543371466	28.846320759629908	30.66641206797785	22.172652629020778
43	18.507299460135055	28.677938444980644	31.356432378009618	21.458329716874687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	17.5
2	25.0
3	96.0
4	167.0
5	167.0
6	276.0
7	385.0
8	344.5
9	304.0
10	411.0
11	518.0
12	518.0
13	1065.0
14	1612.0
15	2700.5
16	3789.0
17	3144.5
18	2500.0
19	2500.0
20	2787.5
21	3075.0
22	2024.5
23	974.0
24	1105.5
25	1237.0
26	1237.0
27	1300.0
28	1363.0
29	1699.5
30	2036.0
31	2310.5
32	2585.0
33	2585.0
34	3055.5
35	3526.0
36	4016.5
37	4507.0
38	5155.0
39	5803.0
40	5803.0
41	6795.0
42	7787.0
43	8503.0
44	9219.0
45	10075.0
46	10931.0
47	10931.0
48	11798.0
49	12665.0
50	11761.5
51	10858.0
52	9546.0
53	8234.0
54	8234.0
55	7218.0
56	6202.0
57	5305.5
58	4409.0
59	3861.5
60	3314.0
61	3314.0
62	2824.5
63	2335.0
64	1909.5
65	1484.0
66	1233.0
67	982.0
68	982.0
69	761.0
70	540.0
71	479.0
72	418.0
73	397.0
74	376.0
75	376.0
76	341.5
77	307.0
78	276.0
79	245.0
80	210.5
81	176.0
82	176.0
83	165.0
84	154.0
85	115.0
86	76.0
87	69.5
88	63.0
89	63.0
90	40.5
91	18.0
92	11.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	115214.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.77667644557085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14156118552405	32.75036019928134
2	11.417162573416814	10.224451889527314
3	4.690922483475159	6.3013175482146275
4	2.6672352633313303	4.777197215616158
5	1.7561883347225182	3.9318138420678044
6	1.1921145980732326	3.202735778638013
7	0.9246157126519219	2.8980853021334214
8	0.7327143383279382	2.6246810283472493
9	0.5272441799608444	2.124741784852535
>10	2.8281222741282055	21.819396948287533
>50	0.09304309058132548	2.676758032877949
>100	0.02132237492488709	1.8383182599336887
>500	0.0019383977204442805	0.601489402329578
>1k	0.005815193161332843	4.228652767892791
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1945	1.6881628968701718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1660	1.44079712534935	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1267	1.0996927456732688	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	693	0.601489402329578	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	381	0.3306889787699412	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	241	0.20917596819830922	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.1779297654798896	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	192	0.16664641449823805	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	188	0.16317461419619145	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.15102331313902825	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	161	0.1397399621573767	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	160	0.13887201208186503	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	151	0.13106046140226013	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	135	0.11717326019407363	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	130	0.11283350981651535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0026038502265349696	0.0
16	0.0	0.0	0.0	0.006943600604093252	0.0
17	0.0	0.0	0.0	0.014755151283698162	0.0
18	0.0	0.0	0.0	0.016491051434721474	0.0
19	0.0	0.0	0.0	0.021698751887791416	0.0
20	0.0	0.0	0.0	0.026906452340861354	0.0
21	0.0	0.0	0.0	0.032114152793931296	0.0
22	0.0	0.0	0.0	0.0520770045306994	0.0
23	0.0	0.0	0.0	0.08158730709809572	0.0
24	0.0	0.0	0.0	0.12672071102470187	0.0
25	0.0	0.0	0.0	0.13973996215737672	0.0
26	0.0	0.0	0.0	0.16317461419619145	0.0
27	0.0	0.0	0.0	0.18053361570642457	0.0
28	0.0	0.0	0.0	0.21264776850035585	0.0
29	0.0	0.0	0.0	0.26038502265349694	0.0
30	0.0	0.0	0.0	0.3818980332251289	0.0
31	0.0	0.0	0.0	0.7342857638828615	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTAAT	25	0.0054760925	29.599998	1
GCGTGAT	25	0.0054760925	29.599998	36
AGGGCGT	25	0.0054760925	29.599998	33
TACCATG	40	0.0019207156	23.125	7
AGATGTG	40	0.0019207156	23.125	5
ATACCAT	60	3.6889636E-5	21.583332	6
TTTTGCG	45	0.003805261	20.555555	2
TATACTC	45	0.003805261	20.555555	5
AGCCTCC	60	9.16823E-4	18.5	19
TTGCGTT	50	0.006997463	18.499998	4
CTACCAC	50	0.006997463	18.499998	3
CGGTGAT	135	1.1150405E-9	17.814816	14
CAGTCGG	135	1.1150405E-9	17.814816	10
TCGGTGA	135	1.1150405E-9	17.814816	13
TCCTCGC	105	4.7053618E-7	17.619047	21
TTCCTCG	105	4.7053618E-7	17.619047	20
GCCTTAT	110	7.6588185E-7	16.818182	26
CGCCTTA	110	7.6588185E-7	16.818182	25
CTCGCCT	110	7.6588185E-7	16.818182	23
CTATACT	90	4.3903623E-5	16.444445	4
>>END_MODULE