FastQCFastQC Report
Fri 10 Feb 2017
ERR1630149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343625
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141624.121353219352492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120503.5067297198981446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86022.503310294652601No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41141.1972353583121134No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18870.5491451436886141No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.41120407420880317No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA13320.3876318661331393No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12040.35038195707530007No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA11840.3445616587850127No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11610.33786831575118226No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA10650.3099308839578028No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10330.30061840669334305No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8650.25172790105492904No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8100.23572208075663875No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA7230.21040378319388867No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA7050.20516551473263003No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6560.190905783921426No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA6360.1850854856311386No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6230.1813022917424518No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA6210.18072026191342305No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6190.18013823208439433No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6150.17897417242633684No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6140.17868315751182248No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.17606402328119317No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5850.1702437249909058No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5750.16733357584576208No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5450.15860312841033103No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5420.15773008366678792No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5310.15452891960712986No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5080.14783557657329938No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5000.14550745725718442No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4930.14347035285558385No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.1428883230265551No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4900.14259730811204074No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.14143324845398325No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4820.1402691887959258No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4800.13968715896689707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4440.12921062204437977No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4170.12135321935249181No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA4060.11815205529283376No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4040.11757002546380502No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4020.11698799563477628No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3970.11553292106220445No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA3940.11465987631866133No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3910.11378683157511821No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3870.11262277191706074No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3700.10767551837031648No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3650.10622044379774463No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3650.10622044379774463No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3490.10156420516551473No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3470.100982175336486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTT200.001840141137.014
ACGTTTA250.00549104729.626
AGCTTGG250.00549104729.617
TGGCGTT250.00549104729.613
CCGTATA250.00549104729.62
AAACCCT250.00549104729.637
GTATACT250.00549104729.64
CTGTGCG250.00549104729.69
TCACGTT250.00549104729.624
CGTATAC405.9282014E-527.753
CGTGGTT358.856693E-426.4285736
TATACTG358.856693E-426.428575
TAAGCGG358.856693E-426.4285718
TACTGTG601.3328518E-624.6666687
AGCCGTC400.00192844823.12512
CGAAAAC400.00192844823.12517
CAGTGGA400.00192844823.12524
ACTGTGC400.00192844823.1258
ACCAATG400.00192844823.1257
GAACAGA400.00192844823.1251
Produced by FastQC (version 0.11.5)