##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630149.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	343625
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70078137504547	31.0	31.0	34.0	30.0	34.0
2	31.759560567479085	31.0	31.0	34.0	30.0	34.0
3	31.78583339396144	33.0	31.0	34.0	30.0	34.0
4	35.516638777737356	37.0	35.0	37.0	33.0	37.0
5	35.51163332120771	37.0	35.0	37.0	33.0	37.0
6	35.65588650418334	37.0	35.0	37.0	33.0	37.0
7	35.62116551473263	37.0	35.0	37.0	33.0	37.0
8	35.69669552564569	37.0	35.0	37.0	33.0	37.0
9	37.25698945070935	39.0	37.0	39.0	34.0	39.0
10	37.19230556566024	39.0	37.0	39.0	34.0	39.0
11	37.317113132048014	39.0	37.0	39.0	34.0	39.0
12	37.1991298654056	39.0	37.0	39.0	34.0	39.0
13	37.270026918879594	39.0	37.0	39.0	34.0	39.0
14	38.14820516551473	40.0	37.0	41.0	33.0	41.0
15	38.15401382320844	40.0	37.0	41.0	33.0	41.0
16	38.04955692979265	40.0	37.0	41.0	33.0	41.0
17	38.26521644234267	40.0	38.0	41.0	34.0	41.0
18	38.38765805747545	40.0	38.0	41.0	34.0	41.0
19	38.4315227355402	40.0	38.0	41.0	34.0	41.0
20	38.477034558021096	40.0	38.0	41.0	34.0	41.0
21	38.45748417606402	40.0	38.0	41.0	34.0	41.0
22	38.400547108039284	40.0	38.0	41.0	34.0	41.0
23	38.305909057839216	40.0	38.0	41.0	34.0	41.0
24	38.231324845398326	40.0	37.0	41.0	34.0	41.0
25	38.163628955983995	40.0	37.0	41.0	33.0	41.0
26	37.831845762095305	40.0	37.0	41.0	33.0	41.0
27	37.619515460167335	40.0	36.0	41.0	33.0	41.0
28	37.456873044743546	39.0	35.0	41.0	33.0	41.0
29	37.25031647871953	39.0	35.0	41.0	33.0	41.0
30	36.92688832302655	38.0	35.0	40.0	32.0	41.0
31	36.53459730811204	38.0	35.0	40.0	31.0	41.0
32	36.20315169152419	38.0	35.0	40.0	31.0	41.0
33	35.94647653692252	37.0	35.0	40.0	31.0	41.0
34	35.686140414696254	37.0	35.0	40.0	30.0	41.0
35	35.41095962168061	37.0	35.0	40.0	30.0	41.0
36	35.04009603492179	37.0	35.0	40.0	27.0	41.0
37	34.742853401236815	36.0	34.0	40.0	25.0	41.0
38	34.37442269916333	35.0	33.0	40.0	24.0	41.0
39	33.962915969443436	35.0	33.0	40.0	21.0	41.0
40	33.48687668242997	35.0	33.0	40.0	19.0	41.0
41	32.986208803201166	35.0	33.0	40.0	15.0	41.0
42	32.48577373590396	35.0	33.0	40.0	12.0	41.0
43	31.381377955620227	35.0	30.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	8.0
11	3.0
12	4.0
13	3.0
14	6.0
15	2.0
16	4.0
17	11.0
18	23.0
19	35.0
20	93.0
21	184.0
22	389.0
23	633.0
24	1089.0
25	1724.0
26	2593.0
27	3714.0
28	5031.0
29	6701.0
30	8792.0
31	10784.0
32	13154.0
33	16857.0
34	21501.0
35	27452.0
36	37855.0
37	63160.0
38	64771.0
39	57045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.79854492542743	19.546016733357586	14.773954165150963	16.881484176064024
2	18.173590396507823	28.336413241178608	31.721207711895232	21.768788650418333
3	22.860676609676243	28.50287377228083	31.410694798108402	17.225754819934522
4	13.487086213168425	18.536777009821755	31.19883594034194	36.777300836667884
5	12.024445252819207	43.93423062931976	29.174827209894506	14.866496907966534
6	28.059658057475446	33.87646416878865	18.47071662422699	19.593161149508912
7	23.56493270280102	36.54623499454347	20.31429610767552	19.574536194979995
8	33.12622771917061	30.166315023644962	19.774463441251363	16.932993815933067
9	29.154747180793017	11.592870134594397	21.96027646416879	37.2921062204438
10	22.523972353583122	31.50760276464169	25.453910512913787	20.514514368861402
11	31.25383775918516	26.94012368133867	17.57671880683885	24.229319752637323
12	20.075954892688248	34.12440887595489	27.45783921425973	18.341797017097125
13	33.51473263004729	20.97664605311022	26.810622044379773	18.697999272462713
14	22.802473626773374	25.398326664241544	30.8577664605311	20.941433248453983
15	27.977882866496905	28.048308475809385	26.677046198617678	17.296762459076028
16	18.38486722444525	27.5463077482721	30.63310294652601	23.435722080756637
17	18.086867951982537	31.068461258639505	30.175627500909425	20.669043288468533
18	16.508694070571117	25.102946526009458	35.17730083666788	23.211058566751547
19	17.487959257911967	28.994397962895597	37.25922153510368	16.25842124408876
20	18.889196071298652	25.816806111313205	38.76900691160422	16.52499090578392
21	20.590469261549654	26.68315751182248	35.89785376500546	16.828519461622406
22	19.957511822480903	28.239505274645328	34.60545652964714	17.19752637322663
23	18.9488541287741	28.998763186613314	35.04226991633321	17.010112768279377
24	17.46933430338305	30.198617679156058	35.271589668970535	17.06045834849036
25	17.28221171335031	30.849327028010187	35.57831938886868	16.290141869770824
26	17.916333212077117	30.761731538741362	35.26547835576574	16.05645689341579
27	16.62830120043652	31.39003273917788	35.83761367770098	16.144052382684613
28	15.582393597671881	33.06249545289196	35.48664969079665	15.868461258639504
29	16.281411422335395	32.88614041469625	34.78763186613314	16.04481629683521
30	15.179919970898508	34.333357584576206	36.057329938159334	14.429392506365952
31	16.43972353583121	32.71938886867952	34.60341942524555	16.237468170243723
32	14.229465260094578	33.71029465260095	34.695962168061115	17.364277919243364
33	15.092615496544198	33.377664605311026	35.89290651145871	15.636813386686068
34	14.014987268097489	33.69341578755911	34.776864314296105	17.514732630047288
35	15.303601309567117	34.502728264823574	34.41920698435795	15.774463441251363
36	14.7463077482721	34.568788650418334	34.17941069479811	16.50549290651146
37	15.179337941069479	34.88512186249545	33.42218988723172	16.513350309203346
38	14.908112040742088	33.81593306656966	33.711458712259	17.56449618042925
39	15.270134594397963	32.755474718079306	34.80945798472172	17.16493270280102
40	14.883375773008368	33.84183339396144	34.261767915605674	17.013022917424518
41	14.768715896689704	32.547690069116044	34.59992724627137	18.08366678792288
42	14.239941797017098	32.918443070207346	34.55889414332485	18.28272098945071
43	14.570825754819936	33.16114950891233	33.76122226264096	18.506802473626774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	109.5
2	152.0
3	550.5
4	949.0
5	949.0
6	1438.5
7	1928.0
8	1923.0
9	1918.0
10	2867.0
11	3816.0
12	3816.0
13	7820.0
14	11824.0
15	19740.5
16	27657.0
17	23389.5
18	19122.0
19	19122.0
20	21743.0
21	24364.0
22	17340.5
23	10317.0
24	9953.0
25	9589.0
26	9589.0
27	9620.0
28	9651.0
29	9019.5
30	8388.0
31	8024.0
32	7660.0
33	7660.0
34	7747.5
35	7835.0
36	7730.5
37	7626.0
38	8498.0
39	9370.0
40	9370.0
41	10681.0
42	11992.0
43	14211.0
44	16430.0
45	20062.0
46	23694.0
47	23694.0
48	27073.0
49	30452.0
50	26106.5
51	21761.0
52	20082.0
53	18403.0
54	18403.0
55	17997.5
56	17592.0
57	15823.5
58	14055.0
59	11667.0
60	9279.0
61	9279.0
62	7678.0
63	6077.0
64	4984.5
65	3892.0
66	3359.5
67	2827.0
68	2827.0
69	2541.5
70	2256.0
71	1722.5
72	1189.0
73	955.5
74	722.0
75	722.0
76	564.0
77	406.0
78	310.0
79	214.0
80	149.0
81	84.0
82	84.0
83	64.5
84	45.0
85	28.5
86	12.0
87	10.5
88	9.0
89	9.0
90	5.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	343625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.14211443525321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94699999999025	21.54971936143385
2	11.338999999998506	6.608848711625852
3	4.3799999999994235	3.8292738367917667
4	2.2649999999997017	2.640275567833593
5	1.5090000000129122	2.198772534158669
6	0.9859999999999225	1.7240474899894442
7	0.7119999999999065	1.452442983452829
8	0.5249999999999309	1.2239688062804734
9	0.46899999999993824	1.230088650311876
>10	3.2249999999995755	18.550704364902337
>50	0.37099999999995115	7.418416650637079
>100	0.2419999999999681	13.917691011986394
>500	0.01799999999999763	3.3157897804426733
>1k	0.008999999999998815	4.194424530665442
>5k	9.999999999998682E-4	2.5068046837201505
>10k+	0.0019999999999997364	7.638731035767564
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14162	4.121353219352492	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12050	3.5067297198981446	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8602	2.503310294652601	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4114	1.1972353583121134	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1887	0.5491451436886141	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1413	0.41120407420880317	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1332	0.3876318661331393	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1204	0.35038195707530007	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	1184	0.3445616587850127	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1161	0.33786831575118226	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	1065	0.3099308839578028	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1033	0.30061840669334305	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	865	0.25172790105492904	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	810	0.23572208075663875	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	723	0.21040378319388867	No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	705	0.20516551473263003	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	656	0.190905783921426	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	636	0.1850854856311386	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	623	0.1813022917424518	No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA	621	0.18072026191342305	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	619	0.18013823208439433	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	615	0.17897417242633684	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	614	0.17868315751182248	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	605	0.17606402328119317	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	585	0.1702437249909058	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	575	0.16733357584576208	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	545	0.15860312841033103	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	542	0.15773008366678792	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	531	0.15452891960712986	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	508	0.14783557657329938	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	500	0.14550745725718442	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	493	0.14347035285558385	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	491	0.1428883230265551	No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA	490	0.14259730811204074	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	486	0.14143324845398325	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	482	0.1402691887959258	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	480	0.13968715896689707	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	444	0.12921062204437977	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	417	0.12135321935249181	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	406	0.11815205529283376	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	404	0.11757002546380502	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	402	0.11698799563477628	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	397	0.11553292106220445	No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA	394	0.11465987631866133	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	391	0.11378683157511821	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	387	0.11262277191706074	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	370	0.10767551837031648	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	365	0.10622044379774463	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	365	0.10622044379774463	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	349	0.10156420516551473	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	347	0.100982175336486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.910149145143689E-4	0.0
10	0.0	0.0	0.0	2.910149145143689E-4	0.0
11	0.0	0.0	0.0	8.730447435431065E-4	0.0
12	0.0	0.0	0.0	0.0011640596580574755	0.0
13	0.0	0.0	0.0	0.00261913423062932	0.0
14	0.0	0.0	0.0	0.0032011640596580574	0.0
15	0.0	0.0	0.0	0.004947253546744271	0.0
16	0.0	0.0	0.0	0.01018552200800291	0.0
17	0.0	0.0	0.0	0.01542379046926155	0.0
18	0.0	0.0	0.0	0.0177519097853765	0.0
19	0.0	0.0	0.0	0.022408148417606404	0.0
20	0.0	0.0	0.0	0.026482357220807565	0.0
21	0.0	0.0	0.0	0.03666787922881048	0.0
22	0.0	0.0	0.0	0.05674790833030193	0.0
23	0.0	0.0	0.0	0.08119316114950892	0.0
24	0.0	0.0	0.0	0.12164423426700618	0.0
25	0.0	0.0	0.0	0.1338668606766097	0.0
26	0.0	0.0	0.0	0.15161877046198619	0.0
27	0.0	0.0	0.0	0.1728628592215351	0.0
28	0.0	0.0	0.0	0.20778464896325938	0.0
29	0.0	0.0	0.0	0.2694798108403056	0.0
30	0.0	0.0	0.0	0.3879228810476537	0.0
31	0.0	0.0	0.0	0.6539105129137869	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTTT	20	0.0018401411	37.0	14
ACGTTTA	25	0.005491047	29.6	26
AGCTTGG	25	0.005491047	29.6	17
TGGCGTT	25	0.005491047	29.6	13
CCGTATA	25	0.005491047	29.6	2
AAACCCT	25	0.005491047	29.6	37
GTATACT	25	0.005491047	29.6	4
CTGTGCG	25	0.005491047	29.6	9
TCACGTT	25	0.005491047	29.6	24
CGTATAC	40	5.9282014E-5	27.75	3
CGTGGTT	35	8.856693E-4	26.42857	36
TATACTG	35	8.856693E-4	26.42857	5
TAAGCGG	35	8.856693E-4	26.42857	18
TACTGTG	60	1.3328518E-6	24.666668	7
AGCCGTC	40	0.001928448	23.125	12
CGAAAAC	40	0.001928448	23.125	17
CAGTGGA	40	0.001928448	23.125	24
ACTGTGC	40	0.001928448	23.125	8
ACCAATG	40	0.001928448	23.125	7
GAACAGA	40	0.001928448	23.125	1
>>END_MODULE