FastQCFastQC Report
Fri 10 Feb 2017
ERR1630148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313897
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC54530.4150249220448787No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT35940.27353742340533543No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG27500.20930103349044862No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG23490.17878113733420506No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT22960.17474733559784367No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG20900.15906878545274097No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG20660.15724215825136978No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG18960.14430354890832386No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT18740.14262914064040028No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT18520.1409547323724767No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC17730.13494208450129652No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG17720.13486597503457273No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT17140.13045162596459237No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG16160.12299289822566No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG15950.12139459942446021No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG15440.11751301662154642No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT15310.11652359355413704No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC14890.11332699595173748No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT14050.10693380074693831No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT13540.10305221794402453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGAC15800.020.0221523
CAGGACA15050.019.9136224
GAACCGT759.267425E-619.7333346
TGCATCA14050.019.48754314
GCATCAG14350.019.08014115
GACAGGC14950.019.0568567
TATATCG609.237774E-418.55
CGTACAC500.007035405418.53
GGTATCA6600.018.2196961
GCTGCAT15350.017.95765512
ACAGGCT16000.017.8062528
CTGCATC15600.017.78846213
GGACAGG17200.017.6395346
TCCAGGA18050.017.2188362
CGAATTA650.001580096517.07692315
CTAGCGG650.001580096517.07692329
CATCAGA16450.016.98176416
ATAAGAC1107.8096855E-716.8181823
ATCAGAA16750.016.45671717
TTAGACT904.446736E-516.4444454
Produced by FastQC (version 0.11.5)