##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630148.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1313897
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.951840212741182	33.0	31.0	34.0	30.0	34.0
2	32.18747207733939	33.0	31.0	34.0	30.0	34.0
3	32.31095512053076	34.0	31.0	34.0	30.0	34.0
4	35.94069930900215	37.0	35.0	37.0	35.0	37.0
5	35.87348475565436	37.0	35.0	37.0	35.0	37.0
6	35.9175437648461	37.0	35.0	37.0	35.0	37.0
7	35.89794481607006	37.0	35.0	37.0	35.0	37.0
8	35.892595081654044	37.0	35.0	37.0	35.0	37.0
9	37.66913997063697	39.0	37.0	39.0	35.0	39.0
10	37.57415535616567	39.0	37.0	39.0	35.0	39.0
11	37.63258383267486	39.0	37.0	39.0	35.0	39.0
12	37.565203360689615	39.0	37.0	39.0	35.0	39.0
13	37.55418879866534	39.0	37.0	39.0	35.0	39.0
14	38.84124250226616	40.0	38.0	41.0	35.0	41.0
15	38.85062527732387	40.0	38.0	41.0	35.0	41.0
16	38.82326164075266	40.0	38.0	41.0	35.0	41.0
17	38.77878935715661	40.0	38.0	41.0	35.0	41.0
18	38.68738188762133	40.0	38.0	41.0	35.0	41.0
19	38.70906547469094	40.0	38.0	41.0	34.0	41.0
20	38.69513896446982	40.0	38.0	41.0	34.0	41.0
21	38.646823913898885	40.0	38.0	41.0	34.0	41.0
22	38.6005310918588	40.0	38.0	41.0	34.0	41.0
23	38.5559994428787	40.0	38.0	41.0	34.0	41.0
24	38.52720418723842	40.0	38.0	41.0	34.0	41.0
25	38.513378902608046	40.0	38.0	41.0	34.0	41.0
26	38.33688942131689	40.0	38.0	41.0	34.0	41.0
27	38.23203721448485	40.0	38.0	41.0	34.0	41.0
28	38.16569792000438	40.0	38.0	41.0	33.0	41.0
29	38.113614689735954	40.0	38.0	41.0	33.0	41.0
30	38.06196147795451	40.0	38.0	41.0	33.0	41.0
31	38.04315482872706	40.0	38.0	41.0	33.0	41.0
32	37.974469079387504	40.0	37.0	41.0	33.0	41.0
33	37.88659917786554	40.0	37.0	41.0	33.0	41.0
34	37.81865016816387	40.0	37.0	41.0	33.0	41.0
35	37.68262276266709	40.0	37.0	41.0	33.0	41.0
36	37.618471615354935	40.0	37.0	41.0	32.0	41.0
37	37.544213891956524	40.0	37.0	41.0	32.0	41.0
38	37.46924073957091	40.0	37.0	41.0	32.0	41.0
39	37.40274542068366	40.0	37.0	41.0	32.0	41.0
40	37.296898463121536	40.0	36.0	41.0	31.0	41.0
41	37.17115801314715	39.0	36.0	41.0	31.0	41.0
42	37.165909504321874	39.0	36.0	41.0	31.0	41.0
43	35.92232876701903	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	3.0
12	1.0
13	1.0
14	0.0
15	1.0
16	7.0
17	11.0
18	34.0
19	100.0
20	179.0
21	306.0
22	654.0
23	1106.0
24	1747.0
25	2784.0
26	4190.0
27	6299.0
28	8769.0
29	12107.0
30	16925.0
31	23271.0
32	30329.0
33	40663.0
34	55915.0
35	76171.0
36	105847.0
37	163919.0
38	313230.0
39	449323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.855870741770474	17.54970138450731	11.677094932098939	27.91733294162328
2	19.247703587115275	19.29161874941491	34.28693421173806	27.17374345173176
3	20.137727690983386	21.53776133136768	28.88909861275275	29.435412364896184
4	15.450678401731643	14.683494977155744	33.16804894143148	36.69777767968113
5	15.937931207697407	35.34927014826885	32.93599117739062	15.776807466643122
6	36.66801887819213	33.35345160237066	15.174020490190632	14.804509029246585
7	31.299028767095137	29.118720873858457	20.291697142165635	19.290553216880777
8	28.593717772397685	32.73772601657512	19.01275366333891	19.65580254768829
9	27.894347882672694	13.761200459396742	17.94714501973899	40.39730663819158
10	17.957115359879808	25.63252675057482	31.77646345185353	24.633894437691843
11	37.242493133023366	20.795541811877186	20.562494624768913	21.399470430330535
12	23.76069052596969	23.42215561798223	28.15654499553618	24.6606088605119
13	31.12017152029421	18.365518758319716	23.65847551215963	26.855834209226447
14	23.59941456598196	18.978047746512853	24.22001115764782	33.202526529857366
15	26.616622155313546	25.725532518911297	20.917469177568712	26.74037614820644
16	25.967636732559708	24.73816440710345	21.828956151052935	27.46524270928391
17	24.98658570648993	25.329002197280303	24.193220625361043	25.491191470868724
18	25.033925794792133	23.705511162594934	24.79889976154904	26.461663281063892
19	26.10432933479565	23.64386249454866	24.363553611888907	25.888254558766782
20	25.82698643805412	24.392779647110846	24.07753423594087	25.70269967889416
21	27.634205725410744	23.59819681451438	23.230664199705153	25.536933260369725
22	27.17785336293484	24.118861676371893	23.363094671804564	25.340190288888703
23	26.505426224430074	24.151817075463295	23.107519082546045	26.235237617560585
24	26.603607436503772	23.88786944486516	23.650864565487247	25.857658553143814
25	26.37094079672912	23.397191712896827	24.23279754805742	25.999069942316634
26	26.87508990430757	23.813814933742904	24.163537933338763	25.14755722861077
27	27.10136334887742	23.66616256829873	23.239569007311836	25.992905075512006
28	25.827823642188086	23.17449541326299	24.586021583122573	26.411659361426352
29	25.752779707998418	23.449250588135904	24.93665789631912	25.861311807546556
30	25.5972880674817	23.971589858261343	24.295740077038	26.13538199721896
31	25.583588363471417	23.9995981420157	24.132333051982005	26.28448044253088
32	25.295285703521664	23.926609163427575	24.575594586181413	26.20251054686935
33	26.381291684203557	23.956139636516408	24.002338082817758	25.66023059646228
34	26.607869566640307	23.833603395091092	24.070836602869175	25.487690435399422
35	26.43540551504418	23.64606966908365	24.78139458420257	25.13713023166961
36	26.29384190693791	24.011395109357885	23.288811832282136	26.40595115142207
37	25.138347983137187	23.529469966062788	24.725454126160574	26.60672792463945
38	26.159965354970748	23.319255618971653	24.844793769983493	25.675985256074107
39	24.59880797353217	23.592488604510095	25.46942416338571	26.339279258572017
40	25.384181560655062	22.405865908819337	26.3913381338111	25.818614396714505
41	24.119698880505855	22.74866294694333	26.75620691728499	26.375431255265823
42	24.105542519695227	23.187281803672587	26.024262175802214	26.682913500829976
43	24.228839855787783	21.350227605360235	27.37939123081946	27.041541308032517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	41.5
2	33.0
3	107.5
4	182.0
5	182.0
6	255.0
7	328.0
8	320.0
9	312.0
10	422.5
11	533.0
12	533.0
13	985.0
14	1437.0
15	2162.0
16	2887.0
17	3079.0
18	3271.0
19	3271.0
20	4134.0
21	4997.0
22	5790.5
23	6584.0
24	8440.0
25	10296.0
26	10296.0
27	12632.0
28	14968.0
29	18398.5
30	21829.0
31	26036.5
32	30244.0
33	30244.0
34	35341.5
35	40439.0
36	45837.5
37	51236.0
38	55081.5
39	58927.0
40	58927.0
41	63561.5
42	68196.0
43	71170.0
44	74144.0
45	76235.5
46	78327.0
47	78327.0
48	80162.5
49	81998.0
50	86869.0
51	91740.0
52	97729.0
53	103718.0
54	103718.0
55	104042.5
56	104367.0
57	99004.0
58	93641.0
59	84397.5
60	75154.0
61	75154.0
62	73968.5
63	72783.0
64	64110.5
65	55438.0
66	46901.5
67	38365.0
68	38365.0
69	33202.0
70	28039.0
71	25929.0
72	23819.0
73	26870.0
74	29921.0
75	29921.0
76	28358.0
77	26795.0
78	20122.0
79	13449.0
80	8401.5
81	3354.0
82	3354.0
83	2219.0
84	1084.0
85	801.5
86	519.0
87	399.0
88	279.0
89	279.0
90	212.0
91	145.0
92	97.5
93	50.0
94	32.0
95	14.0
96	14.0
97	9.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1313897.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.40320544430409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.79365621611524	45.04240288610402
2	10.171934702303906	11.067717067509712
3	2.860044092389501	4.667866990141028
4	1.2037682658330935	2.619554090938058
5	0.6528430017246973	1.775837597285244
6	0.41425127329929995	1.3521958276119828
7	0.29163942023834905	1.1106283506419248
8	0.2169629591666634	0.9442784353078518
9	0.15943058338452873	0.7806181303776394
>10	1.0521577138777691	11.056683324736156
>50	0.1040525466604419	3.928913230578563
>100	0.06382824182802507	7.148838656179106
>500	0.01066140655975579	4.054302692855562
>1k	0.004629294953578172	4.034002178656715
>5k	1.402816652599446E-4	0.4161605410763757
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5453	0.4150249220448787	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3594	0.27353742340533543	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2750	0.20930103349044862	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2349	0.17878113733420506	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2296	0.17474733559784367	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2090	0.15906878545274097	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2066	0.15724215825136978	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1896	0.14430354890832386	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1874	0.14262914064040028	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1852	0.1409547323724767	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1773	0.13494208450129652	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1772	0.13486597503457273	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	1714	0.13045162596459237	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	1616	0.12299289822566	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1595	0.12139459942446021	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1544	0.11751301662154642	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1531	0.11652359355413704	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	1489	0.11332699595173748	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1405	0.10693380074693831	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1354	0.10305221794402453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.805473336189975E-4	0.0	0.0	0.0	0.0
6	3.805473336189975E-4	0.0	0.0	0.0	7.61094667237995E-5
7	5.327662670665965E-4	0.0	0.0	0.0	7.61094667237995E-5
8	5.327662670665965E-4	0.0	0.0	0.0	7.61094667237995E-5
9	5.327662670665965E-4	0.0	0.0	0.0	7.61094667237995E-5
10	5.327662670665965E-4	0.0	0.0	0.0	7.61094667237995E-5
11	6.08875733790396E-4	0.0	0.0	7.61094667237995E-5	7.61094667237995E-5
12	6.849852005141956E-4	0.0	0.0	1.52218933447599E-4	7.61094667237995E-5
13	6.849852005141956E-4	0.0	0.0	2.2832840017139851E-4	7.61094667237995E-5
14	6.849852005141956E-4	0.0	0.0	2.2832840017139851E-4	7.61094667237995E-5
15	8.372041339617946E-4	0.0	0.0	4.5665680034279703E-4	7.61094667237995E-5
16	8.372041339617946E-4	0.0	0.0	6.849852005141956E-4	1.52218933447599E-4
17	8.372041339617946E-4	0.0	0.0	8.372041339617946E-4	1.52218933447599E-4
18	8.372041339617946E-4	0.0	0.0	9.894230674093935E-4	1.52218933447599E-4
19	8.372041339617946E-4	0.0	0.0	0.001065532534133193	1.52218933447599E-4
20	8.372041339617946E-4	0.0	0.0	0.001217751467580792	1.52218933447599E-4
21	0.001065532534133193	0.0	0.0	0.0015982988011997896	2.2832840017139851E-4
22	0.001217751467580792	0.0	0.0	0.0019027366680949877	2.2832840017139851E-4
23	0.001217751467580792	0.0	0.0	0.002435502935161584	2.2832840017139851E-4
24	0.001217751467580792	0.0	0.0	0.004109911203085174	2.2832840017139851E-4
25	0.001217751467580792	0.0	0.0	0.005251553203942166	2.2832840017139851E-4
26	0.001217751467580792	0.0	0.0	0.007915384539275148	2.2832840017139851E-4
27	0.0014460798677521907	0.0	0.0	0.018570709880607078	2.2832840017139851E-4
28	0.00152218933447599	0.0	0.0	0.05061279537132667	2.2832840017139851E-4
29	0.0015982988011997896	0.0	0.0	0.09711567953956816	2.2832840017139851E-4
30	0.0016744082679235892	0.0	0.0	0.16432033865668313	2.2832840017139851E-4
31	0.0016744082679235892	0.0	0.0	0.3710336502785226	2.2832840017139851E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGGAC	1580	0.0	20.022152	3
CAGGACA	1505	0.0	19.913622	4
GAACCGT	75	9.267425E-6	19.733334	6
TGCATCA	1405	0.0	19.487543	14
GCATCAG	1435	0.0	19.080141	15
GACAGGC	1495	0.0	19.056856	7
TATATCG	60	9.237774E-4	18.5	5
CGTACAC	50	0.0070354054	18.5	3
GGTATCA	660	0.0	18.219696	1
GCTGCAT	1535	0.0	17.957655	12
ACAGGCT	1600	0.0	17.806252	8
CTGCATC	1560	0.0	17.788462	13
GGACAGG	1720	0.0	17.639534	6
TCCAGGA	1805	0.0	17.218836	2
CGAATTA	65	0.0015800965	17.076923	15
CTAGCGG	65	0.0015800965	17.076923	29
CATCAGA	1645	0.0	16.981764	16
ATAAGAC	110	7.8096855E-7	16.818182	3
ATCAGAA	1675	0.0	16.456717	17
TTAGACT	90	4.446736E-5	16.444445	4
>>END_MODULE