FastQCFastQC Report
Fri 10 Feb 2017
ERR1630147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612810
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC60690.3762997501255573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53640.3325872235415207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42280.2621511523366051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39670.24596821696294047No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT32810.20343375847123962No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG22860.1417401925831313No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT22310.13832999547373837No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG21790.1351058091157669No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.134733787612924No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG21390.13262566576348112No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT20830.12915346507028105No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG20320.1259912822961167No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT17820.1104903863443307No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG17100.10602612831021634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC451.3235353E-424.66666836
TTAACGG555.1443285E-420.1818235
GGTATCA15550.019.8681681
GACAGGC15600.018.618597
AACGCCG902.1533106E-618.55
CGCGTAA701.21958445E-418.510
CAGGACA17900.017.8798894
CGAATTA1156.409573E-817.69565215
TGCATCA16250.017.64615414
CGCGCTT752.0679175E-417.26666612
TCGCGTA752.0679175E-417.2666669
GCATCAG16500.017.26666615
CCAGGAC18550.017.2533683
GGACAGG17400.017.2241366
ACAGGCT16800.017.1785728
CTTATAC10800.017.1296337
AGGCCAT16550.017.1027225
GCTGCAT16650.016.77777912
ATCAAGC17300.016.3612730
GGCCATC18050.016.09141326
Produced by FastQC (version 0.11.5)