FastQCFastQC Report
Fri 10 Feb 2017
ERR1630146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544388
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC34670.6368619440546082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27070.49725563384938687No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT25080.46070082367723025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22370.41092015253826314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16690.30658280491120304No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT15900.29207109635039713No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG14620.26855845463162303No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG14520.2667215294973438No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG13880.25496520863795674No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT12810.235310109701169No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG12690.23310579954003396No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT12390.22759502413719623No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC12280.22557440648948912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.21161377546896698No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG11410.20959315782125987No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT10960.20132699471700333No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT10620.19508144926045393No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG10560.1939792941798864No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG10540.19361190915303056No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG10340.1899380588844721No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT9900.1818555882936435No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC9640.17707958294451753No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG9280.17046665246111228No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT8530.1566897139540181No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA8390.15411801876602718No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA7650.1405247727723609No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC7590.13942261769179337No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT7560.1388715401515096No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA7440.1366672299903745No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA7410.13611615245009073No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT7140.13115645458753683No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG7050.1295032219666855No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC6910.1269315267786946No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA6630.12178813640271277No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC6480.11903274870129392No Hit
GGGCTGCAGGCTGCCTGCACCAGGGCCCCCGCCCAGCTCCACC6450.11848167116101016No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA6400.11756320859387054No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG6320.11609366848644716No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC6210.11407305083874No Hit
CCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGA6180.11352197329845624No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG6170.11333828078502832No Hit
GGTGCAGGCAGCCTGCAGCCCTTGGCCCTGGAGGGGTCCCTGC5740.10543950270762764No Hit
CACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCG5700.10470473265391596No Hit
GGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGC5660.10396996260020426No Hit
CACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGT5660.10396996260020426No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG5630.10341888505992049No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC5600.10286780751963673No Hit
GGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAG5570.10231672997935295No Hit
CCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG5540.1017656524390692No Hit
GCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGG5490.10084718987192957No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCC5490.10084718987192957No Hit
GCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACAC5480.10066349735850166No Hit
GCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGC5450.10011241981821789No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC5450.10011241981821789No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAC200.001841084737.02
GGTACAC250.00549383329.61
ATACCGA250.00549383329.66
CCATACA358.8633725E-426.4285722
TTATACC502.6995706E-422.24
GTATATA502.6995706E-422.21
CCGCAAG502.6995706E-422.211
CCAGGAC8400.020.4821413
CAGGACA8700.020.201154
ATTAGAG555.138314E-420.1818183
CGGGTAA759.251085E-619.73333424
GCGGGTA759.251085E-619.73333423
CAGATAC1153.0395313E-919.3043483
TGCATCA8600.019.14534814
GCATCAG8650.019.03468115
GACAGGC8850.019.0225987
ACAGGCT8950.018.8100578
GCTGCAT8800.018.71022812
AAGACGG609.228304E-418.55
ACCGACC609.228304E-418.58
Produced by FastQC (version 0.11.5)