##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630146.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544388
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.784278859930783	31.0	31.0	34.0	30.0	34.0
2	31.994867631174824	33.0	31.0	34.0	30.0	34.0
3	32.07597706047892	33.0	31.0	34.0	30.0	34.0
4	35.75137953077584	37.0	35.0	37.0	33.0	37.0
5	35.69629565677421	37.0	35.0	37.0	33.0	37.0
6	35.74349177424925	37.0	35.0	37.0	35.0	37.0
7	35.718364475337445	37.0	35.0	37.0	33.0	37.0
8	35.72385871841407	37.0	35.0	37.0	33.0	37.0
9	37.459431508409445	39.0	37.0	39.0	35.0	39.0
10	37.35315987861599	39.0	37.0	39.0	34.0	39.0
11	37.42793926390736	39.0	37.0	39.0	35.0	39.0
12	37.357886286986485	39.0	37.0	39.0	34.0	39.0
13	37.339289256927046	39.0	37.0	39.0	34.0	39.0
14	38.54048215610925	40.0	38.0	41.0	34.0	41.0
15	38.55486711683579	40.0	38.0	41.0	34.0	41.0
16	38.499968772272716	40.0	38.0	41.0	34.0	41.0
17	38.48483985686679	40.0	38.0	41.0	34.0	41.0
18	38.34142192700794	40.0	38.0	41.0	33.0	41.0
19	38.38552649948199	40.0	38.0	41.0	34.0	41.0
20	38.367155411214064	40.0	38.0	41.0	34.0	41.0
21	38.31437136748055	40.0	38.0	41.0	34.0	41.0
22	38.263183611688724	40.0	38.0	41.0	34.0	41.0
23	38.188204001557715	40.0	38.0	41.0	33.0	41.0
24	38.119596684717514	40.0	38.0	41.0	33.0	41.0
25	38.08705371903863	40.0	37.0	41.0	33.0	41.0
26	37.8633474654107	40.0	37.0	41.0	33.0	41.0
27	37.72888087173119	40.0	37.0	41.0	32.0	41.0
28	37.60252797636979	40.0	37.0	41.0	32.0	41.0
29	37.51199879497711	40.0	37.0	41.0	32.0	41.0
30	37.44050199490069	40.0	36.0	41.0	32.0	41.0
31	37.38416717488262	40.0	36.0	41.0	32.0	41.0
32	37.275114440435864	39.0	36.0	41.0	31.0	41.0
33	37.13344158945458	39.0	36.0	41.0	31.0	41.0
34	37.028150877682826	39.0	35.0	41.0	31.0	41.0
35	36.83257345863612	39.0	35.0	41.0	30.0	41.0
36	36.71754520672756	39.0	35.0	41.0	30.0	41.0
37	36.5895225464191	39.0	35.0	41.0	30.0	41.0
38	36.46328354041603	39.0	35.0	41.0	30.0	41.0
39	36.35957074733462	39.0	35.0	41.0	30.0	41.0
40	36.184149907786356	39.0	35.0	40.0	29.0	41.0
41	35.97548256023278	39.0	35.0	40.0	28.0	41.0
42	35.915479400721544	39.0	35.0	40.0	28.0	41.0
43	34.55643217705019	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	1.0
13	3.0
14	0.0
15	2.0
16	6.0
17	19.0
18	33.0
19	64.0
20	146.0
21	274.0
22	460.0
23	806.0
24	1394.0
25	2113.0
26	3013.0
27	4188.0
28	5814.0
29	7818.0
30	10087.0
31	12697.0
32	16395.0
33	20890.0
34	28024.0
35	35547.0
36	47640.0
37	71387.0
38	121457.0
39	154107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90049009162583	16.81466160165176	11.827226169570233	27.45762213715218
2	20.53370022851349	19.155087915236926	32.92982211217	27.38138974407959
3	21.654591945450672	21.13272151480194	28.2070875919381	29.005598947809286
4	16.436989794043953	14.336098517968802	30.43637993489937	38.79053175308787
5	16.72024364974981	35.310660778709305	30.894692755902042	17.074402815638845
6	37.95638404961167	30.6957537638596	14.819577213311094	16.528284973217634
7	31.62634003688546	28.456909410200076	19.417033439385143	20.49971711352932
8	29.692425255516287	31.323063697215957	18.290630947045123	20.693880100222636
9	28.582555089384776	13.102419597786872	17.71255060728745	40.6024747055409
10	20.26128423109988	25.257353211312523	28.845235383586708	25.636127174000894
11	37.308133169724535	20.796564215228845	18.993071118393498	22.902231496653123
12	24.98603936897948	23.530643585090047	26.1657126902136	25.317604355716878
13	31.39433639242599	17.49946729171106	23.703314547712292	27.402881768150657
14	24.431104285913722	19.097041080993705	23.49739524015959	32.97445939293298
15	28.112485947522725	24.670639323423735	20.394828688361976	26.822046040691568
16	26.362631064608333	23.59034365195412	21.383461795631057	28.663563487806492
17	25.5453830723675	25.002571695187992	23.770178622600056	25.68186660984445
18	25.044086203222705	21.931967640726835	25.072925927830887	27.95102022821958
19	26.40947265553245	22.852634518027585	24.22536867087445	26.512524155565515
20	25.898991160716257	23.196323210651226	24.4454323019611	26.459253326671416
21	29.313467600314482	22.148541114058357	22.96799341646032	25.569997869166844
22	28.191841113323584	23.068473221305393	23.086107702594475	25.65357796277655
23	26.982409604914142	23.450553649235474	22.816079707855426	26.75095703799496
24	27.00114624128379	23.392874200019104	23.766872157358353	25.83910740133875
25	26.487174588712463	22.76482949660904	24.20295818423624	26.545037730442257
26	27.690727936692213	23.305436563627413	24.346789422250307	24.657046077430067
27	28.336958198931644	22.78944429340838	22.783566132978684	26.090031374681296
28	25.951159834529786	22.18454484669023	25.020940946530786	26.843354372249205
29	26.07662182120106	22.395607544618912	25.16367002946428	26.364100604715755
30	25.247250123073982	23.34382829893385	25.107643812868762	26.301277765123405
31	25.581937882539656	23.394711125153382	24.30472383667531	26.718627155631648
32	25.154118018766024	23.271637141156674	24.52515485278882	27.049089987288475
33	26.636883987156217	23.3296839753999	24.060412793816173	25.973019243627704
34	26.834353439091235	23.979037010367605	23.713601328464257	25.4730082220769
35	27.167020580909206	23.122111435226344	24.717664606861284	24.993203377003166
36	26.43849607265406	23.99005856117328	22.811120009992873	26.76032535617978
37	24.951321483941598	23.497211547646163	24.524971160275392	27.02649580813684
38	26.570754682322168	22.77162611960587	24.867006620278183	25.79061257779378
39	24.588161384894597	23.17721918925472	25.233105799540034	27.001513626310647
40	25.615920997523823	22.01187388406798	26.52115770369663	25.851047414711566
41	23.7777100156506	22.58756622115109	27.169408583583767	26.46531517961454
42	24.288375202980227	23.312600571651103	25.61702315260439	26.782001072764277
43	24.094579601313768	20.793808827527425	27.62386386180445	27.48774770935436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	78.0
1	79.0
2	80.0
3	224.5
4	369.0
5	369.0
6	520.0
7	671.0
8	657.0
9	643.0
10	897.5
11	1152.0
12	1152.0
13	2137.0
14	3122.0
15	4642.0
16	6162.0
17	5099.5
18	4037.0
19	4037.0
20	4395.0
21	4753.0
22	3639.0
23	2525.0
24	2774.5
25	3024.0
26	3024.0
27	3513.5
28	4003.0
29	4851.0
30	5699.0
31	6922.5
32	8146.0
33	8146.0
34	9800.5
35	11455.0
36	13450.0
37	15445.0
38	16938.5
39	18432.0
40	18432.0
41	20524.0
42	22616.0
43	23915.5
44	25215.0
45	26780.5
46	28346.0
47	28346.0
48	29970.0
49	31594.0
50	34445.0
51	37296.0
52	41238.5
53	45181.0
54	45181.0
55	45891.5
56	46602.0
57	44524.5
58	42447.0
59	37517.5
60	32588.0
61	32588.0
62	32900.0
63	33212.0
64	29024.5
65	24837.0
66	20957.0
67	17077.0
68	17077.0
69	14768.0
70	12459.0
71	12115.5
72	11772.0
73	14166.5
74	16561.0
75	16561.0
76	16145.5
77	15730.0
78	11948.0
79	8166.0
80	4931.0
81	1696.0
82	1696.0
83	1102.5
84	509.0
85	403.5
86	298.0
87	247.5
88	197.0
89	197.0
90	171.0
91	145.0
92	87.5
93	30.0
94	21.0
95	12.0
96	12.0
97	8.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	544388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.36214882962615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8774649573001	31.029396822509163
2	12.126280014230705	9.788854373682017
3	3.9943357166728086	4.83659918015518
4	1.9152046200769883	3.0920709565894002
5	1.0543402133976598	2.127771830510807
6	0.691855303962031	1.675486004824904
7	0.46973523952692925	1.3271666553814203
8	0.37176273199165993	1.200411417436464
9	0.290573480271486	1.055535305099414
>10	1.8281156523153534	13.91379059151705
>50	0.1876548389854184	5.292508194361172
>100	0.16436920203032168	13.973113577066162
>500	0.01917640690353753	4.957476337478823
>1k	0.00913162233501787	5.729818753388037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	3467	0.6368619440546082	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2707	0.49725563384938687	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	2508	0.46070082367723025	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2237	0.41092015253826314	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1669	0.30658280491120304	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	1590	0.29207109635039713	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1462	0.26855845463162303	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	1452	0.2667215294973438	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1388	0.25496520863795674	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1281	0.235310109701169	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	1269	0.23310579954003396	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1239	0.22759502413719623	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1228	0.22557440648948912	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.21161377546896698	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1141	0.20959315782125987	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1096	0.20132699471700333	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	1062	0.19508144926045393	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	1056	0.1939792941798864	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1054	0.19361190915303056	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1034	0.1899380588844721	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	990	0.1818555882936435	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	964	0.17707958294451753	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	928	0.17046665246111228	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	853	0.1566897139540181	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	839	0.15411801876602718	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	765	0.1405247727723609	No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC	759	0.13942261769179337	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	756	0.1388715401515096	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	744	0.1366672299903745	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	741	0.13611615245009073	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	714	0.13115645458753683	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	705	0.1295032219666855	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	691	0.1269315267786946	No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA	663	0.12178813640271277	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	648	0.11903274870129392	No Hit
GGGCTGCAGGCTGCCTGCACCAGGGCCCCCGCCCAGCTCCACC	645	0.11848167116101016	No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA	640	0.11756320859387054	No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG	632	0.11609366848644716	No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC	621	0.11407305083874	No Hit
CCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGA	618	0.11352197329845624	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	617	0.11333828078502832	No Hit
GGTGCAGGCAGCCTGCAGCCCTTGGCCCTGGAGGGGTCCCTGC	574	0.10543950270762764	No Hit
CACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCG	570	0.10470473265391596	No Hit
GGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGC	566	0.10396996260020426	No Hit
CACTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGT	566	0.10396996260020426	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	563	0.10341888505992049	No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC	560	0.10286780751963673	No Hit
GGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAG	557	0.10231672997935295	No Hit
CCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG	554	0.1017656524390692	No Hit
GCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGG	549	0.10084718987192957	No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCC	549	0.10084718987192957	No Hit
GCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACAC	548	0.10066349735850166	No Hit
GCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGC	545	0.10011241981821789	No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC	545	0.10011241981821789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	1.8369251342792274E-4	0.0	0.0	0.0
11	0.0	1.8369251342792274E-4	0.0	0.0	0.0
12	0.0	1.8369251342792274E-4	0.0	0.0	0.0
13	0.0	1.8369251342792274E-4	0.0	1.8369251342792274E-4	0.0
14	0.0	1.8369251342792274E-4	0.0	5.510775402837682E-4	0.0
15	0.0	1.8369251342792274E-4	0.0	5.510775402837682E-4	0.0
16	0.0	1.8369251342792274E-4	0.0	0.001285847593995459	0.0
17	0.0	1.8369251342792274E-4	0.0	0.001469540107423382	0.0
18	0.0	1.8369251342792274E-4	0.0	0.0016532326208513045	0.0
19	0.0	1.8369251342792274E-4	0.0	0.0020206176477071502	0.0
20	0.0	1.8369251342792274E-4	0.0	0.002571695187990918	0.0
21	0.0	1.8369251342792274E-4	0.0	0.0034901577551305317	0.0
22	0.0	1.8369251342792274E-4	0.0	0.004592312835698068	0.0
23	0.0	1.8369251342792274E-4	0.0	0.008266163104256524	0.0
24	0.0	1.8369251342792274E-4	0.0	0.011572628345959133	0.0
25	0.0	1.8369251342792274E-4	0.0	0.013593245993666282	0.0
26	0.0	1.8369251342792274E-4	0.0	0.0202061764770715	0.0
27	0.0	1.8369251342792274E-4	0.0	0.03710588771244039	0.0
28	0.0	1.8369251342792274E-4	0.0	0.07953885831429054	0.0
29	0.0	1.8369251342792274E-4	0.0	0.13648353747694658	0.0
30	0.0	1.8369251342792274E-4	0.0	0.23163625943261057	0.0
31	0.0	1.8369251342792274E-4	0.0	0.4614355937309419	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTAGAC	20	0.0018410847	37.0	2
GGTACAC	25	0.005493833	29.6	1
ATACCGA	25	0.005493833	29.6	6
CCATACA	35	8.8633725E-4	26.428572	2
TTATACC	50	2.6995706E-4	22.2	4
GTATATA	50	2.6995706E-4	22.2	1
CCGCAAG	50	2.6995706E-4	22.2	11
CCAGGAC	840	0.0	20.482141	3
CAGGACA	870	0.0	20.20115	4
ATTAGAG	55	5.138314E-4	20.181818	3
CGGGTAA	75	9.251085E-6	19.733334	24
GCGGGTA	75	9.251085E-6	19.733334	23
CAGATAC	115	3.0395313E-9	19.304348	3
TGCATCA	860	0.0	19.145348	14
GCATCAG	865	0.0	19.034681	15
GACAGGC	885	0.0	19.022598	7
ACAGGCT	895	0.0	18.810057	8
GCTGCAT	880	0.0	18.710228	12
AAGACGG	60	9.228304E-4	18.5	5
ACCGACC	60	9.228304E-4	18.5	8
>>END_MODULE