FastQCFastQC Report
Fri 10 Feb 2017
ERR1630145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967025
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC56930.2894218426303682No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT32300.16420736899632693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27870.14168604872840965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26890.13670390564431056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21330.10843786937125863No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG20340.10340488809242383No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT20240.10289650614506679No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG20150.10243896239244546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.021.1194691
TTAACGG609.240002E-418.535
TGCATCA14050.017.64412914
GCATCAG14450.017.53979115
GACAGGC15200.017.5263167
GACGGAC3200.017.343757
TAACGGC650.001580474517.07692336
CCAGGAC17300.016.5751463
CTTATAC8600.016.34883737
CGGACCA3400.016.3235289
CAGGACA17100.016.228074
TAATTCG803.3840965E-416.18755
ACAGGCT16250.016.0523078
GCTGCAT15700.016.02547812
TCTAGCG1659.749783E-1015.6969728
TCTATAC2600.015.6538463
AGAGGCC16600.015.60240923
GGACCGT957.0637594E-515.5789486
AAGACGG3650.015.205485
CGCAAGA3650.015.205482
Produced by FastQC (version 0.11.5)