FastQCFastQC Report
Fri 10 Feb 2017
ERR1630144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049911
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40420.3849850130153889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.37126956475358386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28780.27411847289913144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17800.16953817990286796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA358.868884E-426.428576
CGTAGAC701.2190701E-418.53
GTCGGGT2150.017.20930324
GGTATCA18450.016.4444451
TGTACCG904.445367E-516.4444455
CCTAGTA803.3822158E-416.18752
TCGGGTA2200.015.97727325
TTGTCGG2400.015.41666622
GTCATAC750.0041040914.81
AGTGTCG750.0041040914.826
TGTCGGG2400.014.64583323
CGAAATT2301.8189894E-1214.47826113
ACCGAGC908.2731736E-414.3888888
AAGTACG1304.4427907E-614.23076935
TAGAACT1702.4350811E-814.1470594
ACTGTGT2101.3460522E-1014.0952398
GTATAGA1051.6554151E-414.0952391
GGGTAAG2500.014.05999927
GTAGCGA2500.014.0599999
GTCCTAT1601.7824823E-713.8750011
Produced by FastQC (version 0.11.5)