FastQCFastQC Report
Fri 10 Feb 2017
ERR1630143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160489
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17271.076086211516054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.9352665914797899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9830.6125030375913614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.3545414327461695No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2750.17135130756625064No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.14393509835565055No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.13708104605300053No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1950.12150365445606863No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1930.12025746312831409No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1920.11963436746443683No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1820.11340341082566405No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1790.11153412383403224No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1780.11091102817015497No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1680.10468007153138222No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1670.10405697586750493No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC1650.10281078453975039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG200.001837220637.03
CACATGT200.001837220637.028
TAGCGAG200.001837220637.028
ACATGTT200.001837220637.029
GAGGCAC200.001837220637.07
TATTAGA251.2288975E-436.9999962
CCCCTGC250.005482434829.59999810
GCCCAGT250.005482434829.59999819
TGCCCAG250.005482434829.59999818
TGGCGTA250.005482434829.59999822
CGAGGTT250.005482434829.59999822
TTGGTAA250.005482434829.59999833
TTTGGCG250.005482434829.59999820
GTAAGGC250.005482434829.59999816
CTCTAAT250.005482434829.5999981
TGATGTT250.005482434829.59999825
AAATGGA250.005482434829.59999812
TTATACA405.908815E-527.7537
GGCACCG358.8360376E-426.4285729
GCACCGC358.8360376E-426.42857210
Produced by FastQC (version 0.11.5)