##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160489
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64707238502327	31.0	31.0	34.0	30.0	34.0
2	31.8360012212675	33.0	31.0	34.0	30.0	34.0
3	31.9118693493012	33.0	31.0	34.0	30.0	34.0
4	35.621033217229844	37.0	35.0	37.0	33.0	37.0
5	35.566443806117555	37.0	35.0	37.0	33.0	37.0
6	35.65934113864502	37.0	35.0	37.0	33.0	37.0
7	35.66710491061693	37.0	35.0	37.0	33.0	37.0
8	35.67945466667498	37.0	35.0	37.0	33.0	37.0
9	37.30333543108873	39.0	37.0	39.0	34.0	39.0
10	37.21812086809688	39.0	37.0	39.0	34.0	39.0
11	37.30918629937254	39.0	37.0	39.0	34.0	39.0
12	37.17800596925646	39.0	37.0	39.0	33.0	39.0
13	37.25158110524709	39.0	37.0	39.0	34.0	39.0
14	38.36141417791874	40.0	38.0	41.0	34.0	41.0
15	38.360921932344276	40.0	38.0	41.0	34.0	41.0
16	38.257425742574256	40.0	38.0	41.0	33.0	41.0
17	38.3128438709195	40.0	38.0	41.0	34.0	41.0
18	38.336683511019444	40.0	38.0	41.0	34.0	41.0
19	38.3717575659391	40.0	38.0	41.0	34.0	41.0
20	38.33616634161843	40.0	38.0	41.0	34.0	41.0
21	38.297278941235845	40.0	38.0	41.0	34.0	41.0
22	38.28294151001003	40.0	38.0	41.0	34.0	41.0
23	38.23581678495099	40.0	38.0	41.0	34.0	41.0
24	38.234745060409125	40.0	38.0	41.0	34.0	41.0
25	38.19534672158216	40.0	37.0	41.0	34.0	41.0
26	37.972384400176956	40.0	37.0	41.0	33.0	41.0
27	37.844375626990015	40.0	37.0	41.0	33.0	41.0
28	37.776769747459326	40.0	37.0	41.0	33.0	41.0
29	37.69496974870552	40.0	37.0	41.0	33.0	41.0
30	37.57660649639539	39.0	37.0	41.0	33.0	41.0
31	37.3942887051449	39.0	36.0	41.0	32.0	41.0
32	37.251599798117006	39.0	36.0	41.0	32.0	41.0
33	37.17628622522416	39.0	35.0	41.0	31.0	41.0
34	37.07272772588775	39.0	35.0	41.0	31.0	41.0
35	36.94524235305847	39.0	35.0	41.0	31.0	41.0
36	36.79482705979849	39.0	35.0	41.0	31.0	41.0
37	36.680102686165405	39.0	35.0	40.0	30.0	41.0
38	36.54093426963842	39.0	35.0	40.0	30.0	41.0
39	36.40370368062609	39.0	35.0	40.0	30.0	41.0
40	36.25150633376742	39.0	35.0	40.0	30.0	41.0
41	36.03777205914424	39.0	35.0	40.0	29.0	41.0
42	35.91279776184037	39.0	35.0	40.0	29.0	41.0
43	34.8340073151431	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	10.0
19	11.0
20	23.0
21	54.0
22	89.0
23	197.0
24	308.0
25	524.0
26	787.0
27	1142.0
28	1631.0
29	2214.0
30	2961.0
31	3733.0
32	4776.0
33	6453.0
34	8194.0
35	10813.0
36	15085.0
37	23333.0
38	38031.0
39	40118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8833128750257	20.882428079182997	13.380356286100605	22.853902759690694
2	19.65804509966415	22.263831165998916	33.78611618241749	24.292007551919443
3	19.843727607499577	25.922648904286277	30.268118064166394	23.965505424047752
4	13.862632329941615	17.973817520203877	36.69161126307722	31.471938886777284
5	12.719874882390695	38.69486382244266	35.58187788571179	13.003383409454852
6	30.086174130314227	36.74208201185128	17.084660007851006	16.08708384998349
7	27.66357818915938	31.53923321847603	22.95359806591106	17.843590526453525
8	27.653608658537344	32.992292306637836	20.19390737059861	19.160191664226208
9	26.64170130040065	13.767298693368394	21.56097925714535	38.03002074908561
10	19.74091682295983	26.931440784103582	31.49686271333238	21.83077967960421
11	32.76486238932264	24.430957884964076	20.946606932562357	21.857572793150933
12	20.141567334832917	29.580843546909758	28.17140115521936	22.106187963037964
13	30.45629295465733	20.53224831608397	25.90644841702547	23.105010312233237
14	22.43455937790129	22.90998136943965	27.15637831876328	27.49908093389578
15	27.213703119839987	27.378823470767465	23.32122450759865	22.086248901793894
16	22.807793680563776	26.064091619986417	27.280997451538735	23.847117247911072
17	21.16095184093614	28.889830455669856	26.786882590084055	23.162335113309947
18	20.70858438896124	25.97623513137972	28.76209584457502	24.553084635084023
19	22.03453196169208	26.857915495766065	30.223255176367225	20.88429736617463
20	22.195290642972417	25.6559639601468	31.15852176784702	20.990223629033768
21	23.134295808435468	25.88962483410078	29.264934045323976	21.711145312139774
22	23.482606284542864	25.89211721675629	28.47173326520821	22.153543233492638
23	22.45449843914536	26.76569733751223	29.393914847746576	21.385889375595834
24	21.086180361270866	27.803151617867893	28.114076354142654	22.99659166671859
25	22.788477714983582	26.713980397410413	29.587697599212408	20.909844288393597
26	22.517431101196966	26.963218662961324	29.064297241555497	21.455052994286213
27	21.322333617880354	27.457333524416004	28.488556848132895	22.73177600957075
28	21.14724373633084	27.603137909763287	30.461900815632227	20.78771753827365
29	22.20339088660282	27.27601268622772	29.30792764613151	21.21266878103795
30	21.188991145810615	28.69230913022076	29.469309423075725	20.649390300892893
31	22.479422265700453	27.115877100611257	29.274903575946016	21.129797057742277
32	20.382082261089547	27.62058458835185	29.23004068814685	22.767292462411753
33	20.235654780078384	28.00503461296413	30.03009552056527	21.729215086392216
34	21.308002417611178	27.18130214531837	29.15277682582607	22.357918611244383
35	21.61331929291104	28.719725339431363	29.254341419038067	20.412613948619533
36	21.39585890621787	28.410046794484355	27.843029740356034	22.351064558941736
37	21.43760631569765	27.912816454710292	28.605075737277947	22.044501492314115
38	21.13353563172554	28.302874340297468	28.964601935335132	21.598988092641864
39	20.178329979001678	28.015004143586165	29.57461259027098	22.232053287141174
40	20.74659322445775	28.14398494600876	30.180884671223573	20.928537158309915
41	20.01694820205746	27.652362467209592	30.11047486120544	22.22021446952751
42	19.47734735713974	28.031204630846972	30.137267974752163	22.35418003726112
43	20.395790365694847	27.456710428752128	30.313604047629433	21.833895157923596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	20.5
2	33.0
3	101.5
4	170.0
5	170.0
6	275.5
7	381.0
8	354.0
9	327.0
10	416.5
11	506.0
12	506.0
13	1023.5
14	1541.0
15	2387.5
16	3234.0
17	2719.0
18	2204.0
19	2204.0
20	2572.0
21	2940.0
22	1967.5
23	995.0
24	1059.5
25	1124.0
26	1124.0
27	1394.0
28	1664.0
29	2155.5
30	2647.0
31	3434.0
32	4221.0
33	4221.0
34	5356.0
35	6491.0
36	7266.5
37	8042.0
38	8705.0
39	9368.0
40	9368.0
41	10662.0
42	11956.0
43	13024.5
44	14093.0
45	15105.0
46	16117.0
47	16117.0
48	16961.0
49	17805.0
50	16244.0
51	14683.0
52	13169.5
53	11656.0
54	11656.0
55	10620.0
56	9584.0
57	8022.5
58	6461.0
59	5477.5
60	4494.0
61	4494.0
62	3817.5
63	3141.0
64	2408.5
65	1676.0
66	1393.0
67	1110.0
68	1110.0
69	880.0
70	650.0
71	525.0
72	400.0
73	323.5
74	247.0
75	247.0
76	215.5
77	184.0
78	150.0
79	116.0
80	98.0
81	80.0
82	80.0
83	65.0
84	50.0
85	46.5
86	43.0
87	39.0
88	35.0
89	35.0
90	23.0
91	11.0
92	6.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	160489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.38671186187215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.12022694188347	23.07697100735876
2	10.990631182113395	7.338820729146546
3	5.16591392631854	5.1741863928368925
4	3.058863051024598	4.085015172379415
5	1.982008883580307	3.3086379751883306
6	1.4781083199581948	2.960950594744811
7	1.1291105221902877	2.63881013652026
8	0.890224329065731	2.3777330533556817
9	0.6905304020006718	2.074908560711326
>10	5.044604531372475	32.18289103926126
>50	0.36766078160576315	8.214893232557994
>100	0.07465193535142399	3.58778483260535
>500	0.0037325967675711992	0.9670444703375309
>1k	0.0037325967675711992	2.011352802995844
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1727	1.076086211516054	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1501	0.9352665914797899	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.6125030375913614	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	569	0.3545414327461695	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	275	0.17135130756625064	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	231	0.14393509835565055	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	220	0.13708104605300053	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	195	0.12150365445606863	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	193	0.12025746312831409	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	192	0.11963436746443683	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	182	0.11340341082566405	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	179	0.11153412383403224	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	178	0.11091102817015497	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	168	0.10468007153138222	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	167	0.10405697586750493	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	165	0.10281078453975039	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.00124619132775455	0.0
16	0.0	0.0	0.0	0.0031154783193863755	0.0
17	0.0	0.0	0.0	0.0049847653110182	0.0
18	0.0	0.0	0.0	0.006854052302650026	0.0
19	0.0	0.0	0.0	0.009346434958159126	0.0
20	0.0	0.0	0.0	0.010592626285913675	0.0
21	0.0	0.0	0.0	0.015577391596931877	0.0
22	0.0	0.0	0.0	0.031154783193863753	0.0
23	0.0	0.0	0.0	0.050470748774059285	0.0
24	0.0	0.0	0.0	0.07601767099302756	0.0
25	0.0	0.0	0.0	0.08847958427057306	0.0
26	0.0	0.0	0.0	0.10654935852301403	0.0
27	0.0	0.0	0.0	0.12025746312831409	0.0
28	0.0	0.0	0.0	0.1370810460530005	0.0
29	0.0	0.0	0.0	0.17010511623849608	0.0
30	0.0	0.0	0.0	0.24861516988703275	0.0
31	0.0	0.0	0.0	0.4573522172859199	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAG	20	0.0018372206	37.0	3
CACATGT	20	0.0018372206	37.0	28
TAGCGAG	20	0.0018372206	37.0	28
ACATGTT	20	0.0018372206	37.0	29
GAGGCAC	20	0.0018372206	37.0	7
TATTAGA	25	1.2288975E-4	36.999996	2
CCCCTGC	25	0.0054824348	29.599998	10
GCCCAGT	25	0.0054824348	29.599998	19
TGCCCAG	25	0.0054824348	29.599998	18
TGGCGTA	25	0.0054824348	29.599998	22
CGAGGTT	25	0.0054824348	29.599998	22
TTGGTAA	25	0.0054824348	29.599998	33
TTTGGCG	25	0.0054824348	29.599998	20
GTAAGGC	25	0.0054824348	29.599998	16
CTCTAAT	25	0.0054824348	29.599998	1
TGATGTT	25	0.0054824348	29.599998	25
AAATGGA	25	0.0054824348	29.599998	12
TTATACA	40	5.908815E-5	27.75	37
GGCACCG	35	8.8360376E-4	26.428572	9
GCACCGC	35	8.8360376E-4	26.428572	10
>>END_MODULE