FastQCFastQC Report
Fri 10 Feb 2017
ERR1630142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162245
Sequences flagged as poor quality0
Sequence length43
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT89475.514499676415298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62593.8577460014176093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51613.1809917100681067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39882.458011032697464No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18941.1673703349872107No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA14430.8893956670467502No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13990.862276187247681No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.8450183364664551No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10470.6453203488551265No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.6095719436654442No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9230.5688927239668403No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA7470.460414804770563No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6620.40802490061327007No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA6360.39199975345927457No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.3796727171869703No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5150.31742118401183395No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.31618848038460357No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5020.3094086104348362No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.26872939073623225No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4040.24900613270054547No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA3780.23298098554654997No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770.23236463373293476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3630.22373570834232182No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3500.21572313476532404No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.21325772751086322No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3360.2070942093747111No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3160.19476717310240685No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.17689297050756572No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA2700.16641498967610713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2640.16271687879441585No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA2600.160251471539955No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.15963511972633979No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2580.1590187679127246No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2580.1590187679127246No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA2570.15840241609910938No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA2490.1534716015901877No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2380.14669173164042035No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2320.14299362075872907No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.14052821350426825No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA2270.139911861690653No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2270.139911861690653No Hit
GAACTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.13621375080896175No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA2160.13313199174088572No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.13251563992727047No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.12511941816388794No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA2020.12450306635027274No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.11833954821412061No Hit
TATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAA1820.1121760300779685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1760.10847791919627724No Hit
GAGTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAA1690.10416345650097075No Hit
GAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.10416345650097075No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1650.10169804924650991No Hit
GAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.1010816974328947No Hit
AGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.10046534561927947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG200.001837279237.026
TATGTGT200.001837279237.010
CTGATGT200.001837279237.035
GTGAGTG200.001837279237.015
ATGTGTG200.001837279237.011
TATTCTC200.001837279237.05
CTAGTGC200.001837279237.029
CTATGTG200.001837279237.09
CCTAGTG200.001837279237.028
TGAGTGC200.001837279237.016
CCCTAGT200.001837279237.027
TACTATG251.2289562E-437.07
AATTGCC200.001837279237.07
ACTATGT200.001837279237.08
TGATGTA200.001837279237.036
GCGTTAT303.5859828E-430.8333341
TTCACGG250.005482607529.62
GTACTAT250.005482607529.66
TGCTGAT250.005482607529.633
TACACTG250.005482607529.65
Produced by FastQC (version 0.11.5)