##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630142.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162245
Sequences flagged as poor quality	0
Sequence length	43
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73650343616136	31.0	31.0	34.0	30.0	34.0
2	31.764325557027952	31.0	31.0	34.0	30.0	34.0
3	31.73703349872107	33.0	31.0	34.0	28.0	34.0
4	35.495996794970566	37.0	35.0	37.0	33.0	37.0
5	35.497476039323246	37.0	35.0	37.0	33.0	37.0
6	35.66074763474992	37.0	35.0	37.0	33.0	37.0
7	35.60967672347376	37.0	35.0	37.0	33.0	37.0
8	35.739733119664706	37.0	35.0	37.0	35.0	37.0
9	37.30075503097168	39.0	37.0	39.0	34.0	39.0
10	37.24365003544023	39.0	37.0	39.0	34.0	39.0
11	37.359752226570926	39.0	37.0	39.0	34.0	39.0
12	37.358266818700116	39.0	37.0	39.0	34.0	39.0
13	37.40485685229129	39.0	37.0	39.0	34.0	39.0
14	38.316478165737	40.0	38.0	41.0	34.0	41.0
15	38.28178988566674	40.0	38.0	41.0	34.0	41.0
16	38.13724922185583	40.0	37.0	41.0	33.0	41.0
17	38.4139973496872	40.0	38.0	41.0	34.0	41.0
18	38.545631606521	40.0	38.0	41.0	34.0	41.0
19	38.65704336035009	40.0	38.0	41.0	34.0	41.0
20	38.72654935437148	40.0	38.0	41.0	35.0	41.0
21	38.710659804616476	40.0	38.0	41.0	35.0	41.0
22	38.61134703688866	40.0	38.0	41.0	34.0	41.0
23	38.483971771086935	40.0	38.0	41.0	34.0	41.0
24	38.40975684920953	40.0	38.0	41.0	34.0	41.0
25	38.33655274430645	40.0	37.0	41.0	34.0	41.0
26	37.9658849271164	40.0	37.0	41.0	33.0	41.0
27	37.63458350026195	40.0	35.0	41.0	33.0	41.0
28	37.39390428056335	39.0	35.0	41.0	33.0	41.0
29	37.08532158155875	39.0	35.0	41.0	33.0	41.0
30	36.675823600110945	38.0	35.0	40.0	33.0	41.0
31	36.18293938179913	37.0	35.0	40.0	32.0	41.0
32	35.76515146845819	37.0	35.0	40.0	31.0	41.0
33	35.39718943572991	35.0	35.0	40.0	30.0	41.0
34	35.14009676723474	35.0	35.0	40.0	30.0	41.0
35	34.77005146537644	35.0	35.0	40.0	27.0	41.0
36	34.33333538783938	35.0	34.0	40.0	24.0	41.0
37	33.89379641899596	35.0	33.0	40.0	21.0	41.0
38	33.41613609048045	35.0	33.0	40.0	19.0	41.0
39	32.853591790193846	35.0	33.0	40.0	15.0	41.0
40	32.24397053838331	35.0	33.0	39.0	10.0	41.0
41	31.56033776079386	35.0	31.0	39.0	8.0	41.0
42	30.868106875404482	35.0	30.0	39.0	7.0	41.0
43	29.75534531110358	35.0	24.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	3.0
12	1.0
13	4.0
14	0.0
15	0.0
16	1.0
17	2.0
18	10.0
19	25.0
20	59.0
21	119.0
22	208.0
23	345.0
24	624.0
25	942.0
26	1457.0
27	2047.0
28	3062.0
29	3798.0
30	4413.0
31	5443.0
32	6548.0
33	8330.0
34	10504.0
35	12636.0
36	17835.0
37	31621.0
38	27846.0
39	24349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.36894819563006	18.853585626675706	15.524053129526333	13.253413048167895
2	16.20697093901199	32.19082252149527	31.576319763320903	20.025886776171838
3	22.766803291318684	33.295941323307346	31.46969089956547	12.467564485808499
4	13.66328700422201	17.629510924835895	30.416345650097078	38.29085642084502
5	10.69000585534223	46.72871274923727	27.624888286233784	14.956393109186724
6	27.102838300101702	32.78806742888841	19.76270455175814	20.346389719251746
7	21.436716077537056	37.96788807051065	23.381306049493052	17.21408980245924
8	34.44543745569971	29.611390181515606	20.314339424943757	15.628832937840919
9	30.18151560910968	10.894018305648864	24.286726863693797	34.63773922154766
10	20.45979845295695	34.944682424728036	24.686122838916454	19.909396283398564
11	25.392462017319485	31.212055841474314	17.83105796788807	25.56442417331813
12	18.339548214120622	38.24709544207834	28.70781842275571	14.705537921045334
13	30.57659712163703	21.64134487965731	29.80615735461802	17.975900644087645
14	22.670036056581097	25.807883139696138	34.402909180560265	17.1191716231625
15	25.443002866035936	28.151869086874786	30.742395759499523	15.662732287589757
16	15.196770316496655	28.801503898425224	34.80661961847823	21.195106166599896
17	15.021726401429936	33.16342568338007	34.742519029862244	17.072328885327746
18	12.494067613793954	26.495731763690717	39.95993713211501	21.05026349040032
19	13.565903417670807	30.76704983204413	43.51258898579309	12.154457764491971
20	14.989676107121946	28.50442232426269	43.95143147708712	12.554470091528245
21	17.81133470985238	28.20549169465931	41.311596659373166	12.671576936115134
22	17.19806465530525	29.627415328669603	39.76393725538537	13.410582760639771
23	14.735122808098863	31.15720053006256	40.32111929489353	13.78655736694505
24	12.865727757403928	33.274985361644426	40.69524484575796	13.164042035193688
25	12.734444821103885	32.37942617646152	40.43206262134426	14.454066381090328
26	14.590896483712903	32.14891059816944	40.252087891768625	13.00810502634904
27	12.778822151684183	33.68855742858023	39.871182470954416	13.661437948781163
28	12.304847607014084	34.72895928996272	41.731948596258746	11.234244506764462
29	13.106104964713857	34.30984005670437	41.26228851428395	11.32176646429782
30	11.758759900151006	36.430090295540694	41.08354648833554	10.727603315972758
31	12.195753336004191	36.393109186723784	40.343924311997284	11.067213165274739
32	10.473050016949676	37.34352368331844	40.63422601621005	11.549200283521834
33	10.248081604980122	37.53397639372554	41.267219328792876	10.950722672501465
34	10.447163240777837	37.90193842645382	39.15621436716077	12.49468396560757
35	10.164257758328453	38.240931923942185	38.89303214274708	12.70177817498228
36	10.166106813769298	38.39686893278684	37.89762396375851	13.539400289685352
37	9.88135227587907	38.875157940152235	38.25510801565534	12.988381768313353
38	10.418194705537921	37.68005177355234	38.28592560633609	13.61582791457364
39	10.669666245492927	37.40639156830719	39.32447841227773	12.599463773922153
40	10.437301611759993	37.63259268390397	38.89241579093346	13.03768991340257
41	9.776572467564487	38.04123393633086	39.02678048630158	13.155413109803076
42	9.628648032296836	37.94323399796604	38.40549785817745	14.022620111559677
43	9.24835896329625	38.202101759684425	37.928441554439274	14.621097722580048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	135.0
2	208.0
3	674.0
4	1140.0
5	1140.0
6	1663.0
7	2186.0
8	2147.5
9	2109.0
10	2834.5
11	3560.0
12	3560.0
13	7108.5
14	10657.0
15	15340.0
16	20023.0
17	15925.5
18	11828.0
19	11828.0
20	12426.0
21	13024.0
22	8774.5
23	4525.0
24	4762.0
25	4999.0
26	4999.0
27	4899.5
28	4800.0
29	4585.5
30	4371.0
31	4331.5
32	4292.0
33	4292.0
34	4326.0
35	4360.0
36	4212.5
37	4065.0
38	4519.5
39	4974.0
40	4974.0
41	5875.0
42	6776.0
43	7237.5
44	7699.0
45	8817.0
46	9935.0
47	9935.0
48	10837.0
49	11739.0
50	9438.0
51	7137.0
52	5961.5
53	4786.0
54	4786.0
55	4229.0
56	3672.0
57	3446.5
58	3221.0
59	2690.5
60	2160.0
61	2160.0
62	1789.5
63	1419.0
64	1215.5
65	1012.0
66	792.0
67	572.0
68	572.0
69	479.5
70	387.0
71	305.5
72	224.0
73	179.5
74	135.0
75	135.0
76	114.5
77	94.0
78	81.0
79	68.0
80	42.0
81	16.0
82	16.0
83	10.0
84	4.0
85	2.0
86	0.0
87	2.0
88	4.0
89	4.0
90	2.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	162245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.34984128940799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25027626414663	24.019846528398407
2	11.233471782951645	7.268020586150575
3	4.633616583469878	4.496902832136584
4	2.3625347711770757	3.0571049955314495
5	1.575658270776969	2.5486147492989
6	1.0669511869831956	2.0709420937471106
7	0.804023930190908	1.8207032574193351
8	0.5639599131196891	1.459521094640821
9	0.4744122242121709	1.381244414311689
>10	2.6673779674579885	15.76381398502265
>50	0.1886217276988149	4.255292921199421
>100	0.14480051823343368	8.675768128447718
>500	0.017147429790801357	3.761595118493636
>1k	0.011431619860534236	6.8673919073006875
>5k	0.005715809930267118	12.553237387901014
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8947	5.514499676415298	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6259	3.8577460014176093	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5161	3.1809917100681067	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3988	2.458011032697464	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1894	1.1673703349872107	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1443	0.8893956670467502	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1399	0.862276187247681	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1371	0.8450183364664551	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1047	0.6453203488551265	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	989	0.6095719436654442	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.5688927239668403	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	747	0.460414804770563	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	662	0.40802490061327007	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	636	0.39199975345927457	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	616	0.3796727171869703	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	515	0.31742118401183395	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	513	0.31618848038460357	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	502	0.3094086104348362	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	436	0.26872939073623225	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	404	0.24900613270054547	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	378	0.23298098554654997	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	377	0.23236463373293476	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	363	0.22373570834232182	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	350	0.21572313476532404	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	346	0.21325772751086322	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	336	0.2070942093747111	No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	316	0.19476717310240685	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	287	0.17689297050756572	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	270	0.16641498967610713	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	264	0.16271687879441585	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	260	0.160251471539955	No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	259	0.15963511972633979	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	258	0.1590187679127246	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	258	0.1590187679127246	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	257	0.15840241609910938	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	249	0.1534716015901877	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	238	0.14669173164042035	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	232	0.14299362075872907	No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	228	0.14052821350426825	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	227	0.139911861690653	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	227	0.139911861690653	No Hit
GAACTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	221	0.13621375080896175	No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA	216	0.13313199174088572	No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	215	0.13251563992727047	No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	203	0.12511941816388794	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	202	0.12450306635027274	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	192	0.11833954821412061	No Hit
TATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAA	182	0.1121760300779685	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	176	0.10847791919627724	No Hit
GAGTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAA	169	0.10416345650097075	No Hit
GAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	169	0.10416345650097075	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	165	0.10169804924650991	No Hit
GAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	164	0.1010816974328947	No Hit
AGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	163	0.10046534561927947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.163518136152116E-4	0.0
12	0.0	0.0	0.0	6.163518136152116E-4	0.0
13	0.0	0.0	0.0	6.163518136152116E-4	0.0
14	0.0	0.0	0.0	0.0018490554408456348	0.0
15	0.0	0.0	0.0	0.0036981108816912696	0.0
16	0.0	0.0	0.0	0.008628925390612961	0.0
17	0.0	0.0	0.0	0.012943388085919444	0.0
18	0.0	0.0	0.0	0.018490554408456348	0.0
19	0.0	0.0	0.0	0.02342136891737804	0.0
20	0.0	0.0	0.0	0.03143394249437579	0.0
21	0.0	0.0	0.0	0.042528275139449595	0.0
22	0.0	0.0	0.0	0.06594964405682764	0.0
23	0.0	0.0	0.0	0.08937101297420567	0.0
24	0.0	0.0	0.0	0.128201177231964	0.0
25	0.0	0.0	0.0	0.14545902801318994	0.0
26	0.0	0.0	0.0	0.16394958242164628	0.0
27	0.0	0.0	0.0	0.19045271040710038	0.0
28	0.0	0.0	0.0	0.24900613270054547	0.0
29	0.0	0.0	0.0	0.3414589047428272	0.0
30	0.0	0.0	0.0	0.6163518136152115	0.0
31	0.0	0.0	0.0	1.1057351536256896	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCTAG	20	0.0018372792	37.0	26
TATGTGT	20	0.0018372792	37.0	10
CTGATGT	20	0.0018372792	37.0	35
GTGAGTG	20	0.0018372792	37.0	15
ATGTGTG	20	0.0018372792	37.0	11
TATTCTC	20	0.0018372792	37.0	5
CTAGTGC	20	0.0018372792	37.0	29
CTATGTG	20	0.0018372792	37.0	9
CCTAGTG	20	0.0018372792	37.0	28
TGAGTGC	20	0.0018372792	37.0	16
CCCTAGT	20	0.0018372792	37.0	27
TACTATG	25	1.2289562E-4	37.0	7
AATTGCC	20	0.0018372792	37.0	7
ACTATGT	20	0.0018372792	37.0	8
TGATGTA	20	0.0018372792	37.0	36
GCGTTAT	30	3.5859828E-4	30.833334	1
TTCACGG	25	0.0054826075	29.6	2
GTACTAT	25	0.0054826075	29.6	6
TGCTGAT	25	0.0054826075	29.6	33
TACACTG	25	0.0054826075	29.6	5
>>END_MODULE