FastQCFastQC Report
Fri 10 Feb 2017
ERR1630141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736263
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73870.425453977882383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.4181394178186139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53440.30778747228962433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.21546274959496342No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA34330.19772350156629495No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT33550.1932310945979958No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA32650.18804754809611215No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC29540.17013551518404757No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA22700.1307405617697319No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG21350.12296524201690642No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA20200.11634182148672176No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA18150.10453485445465348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG2000.020.355
GGTATCA29250.018.7846151
ACGGACC2200.018.58
GACGGAC2200.018.57
GCTTTAT11100.018.1666661
CGGACCA2350.017.3191499
GTATACG1005.8812675E-616.6500021
TTATTGA13050.016.3026834
TCTAGCG2003.6379788E-1215.72528
CTAGCGG2003.6379788E-1215.72529
CGCAAGA2850.015.5789472
CTTAACG855.366109E-415.23529430
TTTATTG16550.015.2024173
GCGAAAG2800.015.19642818
CGAATTA1601.0973963E-815.0312515
CTTATAC10850.014.83410237
CGCGCAA1651.6465492E-814.57575721
CGCAATA2059.276846E-1114.43902536
TATTGAG13750.014.3963635
ATTACGT908.277014E-414.38888817
Produced by FastQC (version 0.11.5)