##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630141.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1736263
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835705765773962	33.0	31.0	34.0	30.0	34.0
2	32.04195274563819	33.0	31.0	34.0	30.0	34.0
3	32.1675281912936	34.0	31.0	34.0	30.0	34.0
4	35.804988645153415	37.0	35.0	37.0	35.0	37.0
5	35.73669714783993	37.0	35.0	37.0	35.0	37.0
6	35.8001293582827	37.0	35.0	37.0	35.0	37.0
7	35.77738856382933	37.0	35.0	37.0	35.0	37.0
8	35.78402292740213	37.0	35.0	37.0	35.0	37.0
9	37.510613311462606	39.0	37.0	39.0	35.0	39.0
10	37.42136358374279	39.0	37.0	39.0	34.0	39.0
11	37.48288364147597	39.0	37.0	39.0	35.0	39.0
12	37.41958793109109	39.0	37.0	39.0	34.0	39.0
13	37.428850928689954	39.0	37.0	39.0	34.0	39.0
14	38.65135178253525	40.0	38.0	41.0	34.0	41.0
15	38.660321621781954	40.0	38.0	41.0	34.0	41.0
16	38.60835253645329	40.0	38.0	41.0	34.0	41.0
17	38.599739210016	40.0	38.0	41.0	34.0	41.0
18	38.56699935436048	40.0	38.0	41.0	34.0	41.0
19	38.58575400155391	40.0	38.0	41.0	34.0	41.0
20	38.57576761124323	40.0	38.0	41.0	34.0	41.0
21	38.510114539099206	40.0	38.0	41.0	34.0	41.0
22	38.46693041319201	40.0	38.0	41.0	34.0	41.0
23	38.421023197522494	40.0	38.0	41.0	34.0	41.0
24	38.37246891743935	40.0	38.0	41.0	34.0	41.0
25	38.35850501911289	40.0	38.0	41.0	34.0	41.0
26	38.18892126365648	40.0	38.0	41.0	33.0	41.0
27	38.06407266641056	40.0	38.0	41.0	33.0	41.0
28	37.974658217101904	40.0	37.0	41.0	33.0	41.0
29	37.91626441385896	40.0	37.0	41.0	33.0	41.0
30	37.855763210988194	40.0	37.0	41.0	33.0	41.0
31	37.779376165938	40.0	37.0	41.0	33.0	41.0
32	37.69958929033217	40.0	37.0	41.0	33.0	41.0
33	37.57877925176082	40.0	37.0	41.0	32.0	41.0
34	37.53098234541656	40.0	37.0	41.0	32.0	41.0
35	37.38584592311188	40.0	36.0	41.0	32.0	41.0
36	37.29990675375793	40.0	36.0	41.0	31.0	41.0
37	37.23441609940429	40.0	36.0	41.0	31.0	41.0
38	37.12085093099375	39.0	36.0	41.0	31.0	41.0
39	37.0236070226688	39.0	36.0	41.0	31.0	41.0
40	36.88759421815704	39.0	36.0	41.0	30.0	41.0
41	36.76754500902225	39.0	35.0	41.0	30.0	41.0
42	36.709155237426586	39.0	35.0	41.0	30.0	41.0
43	35.51452055362581	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	7.0
12	2.0
13	4.0
14	4.0
15	8.0
16	14.0
17	20.0
18	59.0
19	130.0
20	265.0
21	500.0
22	983.0
23	1632.0
24	2875.0
25	4541.0
26	6900.0
27	10263.0
28	14716.0
29	20254.0
30	27462.0
31	35730.0
32	46051.0
33	59243.0
34	78084.0
35	105139.0
36	146466.0
37	222278.0
38	399643.0
39	552983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11259296546664	19.695345693595957	12.557602160502181	24.634459180435222
2	19.088064423419723	21.513330641728817	33.05887414521878	26.339730789632675
3	20.727677776926654	23.149776272373483	29.52369543093414	26.598850519765726
4	14.696391042140505	15.423988186121573	34.957952798625556	34.92166797311237
5	14.626931519015265	36.6324686985785	34.06528849604006	14.675311286366178
6	34.40083673959533	34.911704044836526	15.611805354373157	15.075653861194994
7	28.98207241644843	30.663672496620613	21.409083762079824	18.94517132485113
8	28.38112659199672	31.53796400660499	20.37081939775253	19.71009000364576
9	26.65823092469286	14.198425008192883	20.58599417254183	38.557349894572425
10	18.009771561105662	26.659670798721162	32.682663859104295	22.64789378106888
11	34.628855190717076	22.628253899322857	21.34883943273571	21.39405147722436
12	22.457139269799566	26.18197819109202	28.33545378781901	23.025428751289407
13	30.688150355101733	19.8909957765615	24.91552259075958	24.505331277577188
14	22.938748334785686	21.442143269769616	25.44752724673624	30.17158114870846
15	25.485539921083383	28.163360043956477	22.446829771756928	23.904270263203212
16	24.354720454216903	26.571089748500082	24.607735118469957	24.46645467881306
17	23.616641027309804	26.544884041185007	25.694840009837222	24.14363492166797
18	23.132094619305946	25.002433387107832	27.463293291396525	24.4021787021897
19	24.394000217708953	25.20009929371299	27.727308593225796	22.67859189535226
20	25.147630284121703	24.85648775559924	26.298320012578742	23.69756194770032
21	24.81369469947813	24.933434623671644	26.610196727108736	23.642673949741486
22	24.724652889567995	25.224749937077505	27.129069731947293	22.921527441407203
23	24.25415965208036	24.956242228279933	26.432861841783183	24.356736277856523
24	23.544013781322302	25.72905141674965	27.309169175407185	23.41776562652087
25	24.05378678230199	25.872002110279375	26.391508659690384	23.682702447728254
26	23.93969116429942	26.200984528265593	26.454344762285437	23.404979545149555
27	24.2078533033302	24.815940902962282	26.796919591098817	24.179286202608708
28	23.551501126269464	25.60648933946067	26.759367676440725	24.08264185782914
29	23.513315667038924	26.019963565427588	27.224792557348742	23.24192821018475
30	24.016522842449557	26.06177750720945	26.28507317151837	23.63662647882262
31	23.10565853214634	26.79991452907768	26.948567123759474	23.145859815016504
32	23.15904906111574	25.80046916855338	26.66468156033965	24.375800209991226
33	22.299962620870225	26.290371907942518	27.303352084332843	24.10631338685441
34	24.477916076078333	24.706856046578196	26.87893481575084	23.93629306159263
35	23.285642785683965	25.18120814646168	27.212467235666487	24.320681832187866
36	22.66476910468057	26.213252254986713	27.33341665404377	23.788561986288943
37	23.409587142040117	24.89674663343053	27.831785852719314	23.86188037181003
38	23.543668211555506	25.214670818879398	27.30404322386643	23.937617745698663
39	23.277176326397555	24.735019982571764	27.904701073512477	24.083102617518197
40	22.30289996388796	24.463056576106272	28.694558370477285	24.539485089528487
41	21.605252199695553	24.766582021272125	29.08027182517856	24.547893953853766
42	22.033413140751144	24.60393385103524	29.37734663469762	23.985306373515993
43	22.186961307129163	24.565921176688093	29.064893970556305	24.182223545626442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1458.0
1	1097.5
2	737.0
3	1266.5
4	1796.0
5	1796.0
6	2307.5
7	2819.0
8	2526.5
9	2234.0
10	3146.0
11	4058.0
12	4058.0
13	7303.0
14	10548.0
15	14399.5
16	18251.0
17	17417.0
18	16583.0
19	16583.0
20	20050.5
21	23518.0
22	22529.5
23	21541.0
24	25457.5
25	29374.0
26	29374.0
27	32622.0
28	35870.0
29	40160.0
30	44450.0
31	49900.5
32	55351.0
33	55351.0
34	60313.0
35	65275.0
36	70902.5
37	76530.0
38	79526.0
39	82522.0
40	82522.0
41	86022.5
42	89523.0
43	90670.0
44	91817.0
45	95142.0
46	98467.0
47	98467.0
48	105151.0
49	111835.0
50	110896.5
51	109958.0
52	113068.5
53	116179.0
54	116179.0
55	112007.5
56	107836.0
57	106418.5
58	105001.0
59	104016.0
60	103031.0
61	103031.0
62	96589.5
63	90148.0
64	83257.5
65	76367.0
66	65468.5
67	54570.0
68	54570.0
69	47832.5
70	41095.0
71	34276.5
72	27458.0
73	18574.5
74	9691.0
75	9691.0
76	6969.0
77	4247.0
78	3370.5
79	2494.0
80	1972.0
81	1450.0
82	1450.0
83	1068.5
84	687.0
85	643.0
86	599.0
87	542.0
88	485.0
89	485.0
90	393.0
91	301.0
92	183.5
93	66.0
94	48.0
95	30.0
96	30.0
97	19.5
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1736263.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46570962330239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.625734330877	40.68834896907561
2	11.695633181181218	11.804568559643036
3	3.3636004878106798	5.0923945651995615
4	1.3398492395313781	2.7046577064477226
5	0.6637122033851951	1.674735366473974
6	0.406091799180804	1.229622649071773
7	0.2653015442987695	0.9372041488036778
8	0.2048706989075352	0.8271156161112547
9	0.15574858461470165	0.7073966560865288
>10	1.0407441104921822	10.356636364195056
>50	0.12120527479614483	4.311598121741507
>100	0.10063990406426757	10.573850348353215
>500	0.011131007914624664	3.7387176089067387
>1k	0.005393374968941847	4.195459643935355
>5k	3.4425797674096897E-4	1.1576936759549972
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7387	0.425453977882383	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7260	0.4181394178186139	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5344	0.30778747228962433	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3741	0.21546274959496342	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	3433	0.19772350156629495	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	3355	0.1932310945979958	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3265	0.18804754809611215	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2954	0.17013551518404757	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	2270	0.1307405617697319	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	2135	0.12296524201690642	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	2020	0.11634182148672176	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	1815	0.10453485445465348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1518992226408097E-4	0.0
2	0.0	0.0	0.0	1.1518992226408097E-4	0.0
3	0.0	0.0	0.0	1.1518992226408097E-4	0.0
4	0.0	0.0	0.0	1.1518992226408097E-4	0.0
5	0.0	0.0	0.0	1.1518992226408097E-4	0.0
6	0.0	0.0	0.0	1.1518992226408097E-4	0.0
7	0.0	0.0	0.0	1.1518992226408097E-4	0.0
8	0.0	0.0	0.0	1.1518992226408097E-4	0.0
9	0.0	0.0	0.0	1.7278488339612144E-4	0.0
10	0.0	0.0	0.0	2.3037984452816193E-4	0.0
11	0.0	0.0	0.0	2.3037984452816193E-4	0.0
12	0.0	0.0	0.0	2.879748056602024E-4	0.0
13	0.0	0.0	0.0	4.6075968905632386E-4	0.0
14	0.0	0.0	0.0	5.183546501883644E-4	0.0
15	0.0	0.0	0.0	5.759496113204047E-4	0.0
16	0.0	0.0	0.0	8.063294558485667E-4	0.0
17	0.0	0.0	0.0	0.001439874028301012	0.0
18	0.0	0.0	0.0	0.0016126589116971335	0.0
19	0.0	0.0	0.0	0.002131013561885498	0.0
20	0.0	0.0	0.0	0.002649368212073862	0.0
21	0.0	0.0	0.0	0.0032829127845263074	0.0
22	0.0	0.0	0.0	0.004492406968299158	0.0
23	0.0	0.0	0.0	0.006162660841128331	0.0
24	0.0	0.0	0.0	0.010597472848295448	0.0
25	0.0	0.0	0.0	0.01324684106036931	0.0
26	5.759496113204048E-5	0.0	0.0	0.018660767406781114	0.0
27	5.759496113204048E-5	0.0	0.0	0.040028497986768136	0.0
28	5.759496113204048E-5	0.0	0.0	0.08812029053202193	0.0
29	5.759496113204048E-5	0.0	0.0	0.1614962710142415	0.0
30	5.759496113204048E-5	0.0	0.0	0.2516323851858849	0.0
31	5.759496113204048E-5	0.0	0.0	0.4771166580178233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	200	0.0	20.35	5
GGTATCA	2925	0.0	18.784615	1
ACGGACC	220	0.0	18.5	8
GACGGAC	220	0.0	18.5	7
GCTTTAT	1110	0.0	18.166666	1
CGGACCA	235	0.0	17.319149	9
GTATACG	100	5.8812675E-6	16.650002	1
TTATTGA	1305	0.0	16.302683	4
TCTAGCG	200	3.6379788E-12	15.725	28
CTAGCGG	200	3.6379788E-12	15.725	29
CGCAAGA	285	0.0	15.578947	2
CTTAACG	85	5.366109E-4	15.235294	30
TTTATTG	1655	0.0	15.202417	3
GCGAAAG	280	0.0	15.196428	18
CGAATTA	160	1.0973963E-8	15.03125	15
CTTATAC	1085	0.0	14.834102	37
CGCGCAA	165	1.6465492E-8	14.575757	21
CGCAATA	205	9.276846E-11	14.439025	36
TATTGAG	1375	0.0	14.396363	5
ATTACGT	90	8.277014E-4	14.388888	17
>>END_MODULE