FastQCFastQC Report
Fri 10 Feb 2017
ERR1630140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528646
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36860.24112842345448193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.23975465869795887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28420.18591616371612527No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17470.11428414426884968No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15990.10460237360382979No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.10427528675703858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATTC1303.274181E-1119.9230773
GGTATCA15200.019.4736841
GATCGGA701.2195312E-418.517
TACGGGT609.2387194E-418.512
GTAATAC1451.546141E-1017.8620683
ACAACGC650.001580256217.0769238
AAGACGG3900.017.0769235
GTTCTAG2550.015.9607841
GTATTAA1902.7284841E-1115.5789481
GACGGAC4000.015.2624997
TACGCAG855.365589E-415.2352945
CTAGACT1101.4524128E-515.1363644
CTAGCGG3250.014.80000129
AGACGGA4400.014.7159096
CTAGAAC2650.014.66037753
CGCAGTT1152.2106775E-514.4782627
TCTATAC1152.2106775E-514.478263
GTCCTAA2059.276846E-1114.4390251
ACGGACC4400.014.2954548
GTCTAGG1304.445981E-614.2307691
Produced by FastQC (version 0.11.5)