##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630140.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1528646
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.878256967276922	33.0	31.0	34.0	30.0	34.0
2	32.08552143530942	33.0	31.0	34.0	30.0	34.0
3	32.21535070905887	34.0	31.0	34.0	30.0	34.0
4	35.8431736320901	37.0	35.0	37.0	35.0	37.0
5	35.77459594961816	37.0	35.0	37.0	35.0	37.0
6	35.8273027241101	37.0	35.0	37.0	35.0	37.0
7	35.80406778286144	37.0	35.0	37.0	35.0	37.0
8	35.80307867223674	37.0	35.0	37.0	35.0	37.0
9	37.55207484270394	39.0	37.0	39.0	35.0	39.0
10	37.449461157128596	39.0	37.0	39.0	35.0	39.0
11	37.51278124562521	39.0	37.0	39.0	35.0	39.0
12	37.43471019451201	39.0	37.0	39.0	34.0	39.0
13	37.442421594011954	39.0	37.0	39.0	35.0	39.0
14	38.684974153597366	40.0	38.0	41.0	35.0	41.0
15	38.68213373141983	40.0	38.0	41.0	34.0	41.0
16	38.64744290044916	40.0	38.0	41.0	34.0	41.0
17	38.6155329618499	40.0	38.0	41.0	34.0	41.0
18	38.57329689149744	40.0	38.0	41.0	34.0	41.0
19	38.58764619146617	40.0	38.0	41.0	34.0	41.0
20	38.56930054440335	40.0	38.0	41.0	34.0	41.0
21	38.51120141615521	40.0	38.0	41.0	34.0	41.0
22	38.466269496011506	40.0	38.0	41.0	34.0	41.0
23	38.39824131944217	40.0	38.0	41.0	34.0	41.0
24	38.35283119832845	40.0	38.0	41.0	34.0	41.0
25	38.3450897068386	40.0	38.0	41.0	34.0	41.0
26	38.175328362485494	40.0	38.0	41.0	33.0	41.0
27	38.057314119815835	40.0	38.0	41.0	33.0	41.0
28	37.96790493024546	40.0	37.0	41.0	33.0	41.0
29	37.90474053508792	40.0	37.0	41.0	33.0	41.0
30	37.84167688267918	40.0	37.0	41.0	33.0	41.0
31	37.793669037828245	40.0	37.0	41.0	33.0	41.0
32	37.69735962413796	40.0	37.0	41.0	33.0	41.0
33	37.586567459045455	40.0	37.0	41.0	32.0	41.0
34	37.53679465356924	40.0	37.0	41.0	32.0	41.0
35	37.40025617441841	40.0	37.0	41.0	31.0	41.0
36	37.31362329800359	40.0	36.0	41.0	31.0	41.0
37	37.251580810730545	40.0	36.0	41.0	31.0	41.0
38	37.14271453299194	39.0	36.0	41.0	31.0	41.0
39	37.061549894481786	39.0	36.0	41.0	31.0	41.0
40	36.935369601595134	39.0	36.0	41.0	31.0	41.0
41	36.81144489960396	39.0	35.0	41.0	30.0	41.0
42	36.75214470845441	39.0	35.0	41.0	30.0	41.0
43	35.512248094064944	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	0.0
13	0.0
14	3.0
15	5.0
16	15.0
17	39.0
18	67.0
19	136.0
20	262.0
21	482.0
22	976.0
23	1666.0
24	2484.0
25	4168.0
26	5998.0
27	9129.0
28	12813.0
29	17782.0
30	23919.0
31	30997.0
32	39913.0
33	51600.0
34	68052.0
35	91122.0
36	127425.0
37	193767.0
38	351305.0
39	494514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89135614131722	18.898358416533323	12.062177901227622	26.148107540921835
2	19.225445263324538	20.62877867079756	33.62649037121741	26.519285694660503
3	20.016341258865687	22.464390054989842	29.21389255589587	28.3053761302486
4	14.634192612285643	15.376156415546829	34.001855236595006	35.987795735572526
5	15.254938030126006	35.90994906603622	33.403220889597726	15.431892014240054
6	35.397534811853106	34.475607825487394	15.491029316139905	14.6358280465196
7	30.107428403960107	30.020946641668512	20.849104370796116	19.022520583575268
8	27.965205809585736	32.76631738152587	19.493591060323972	19.774885748564415
9	26.99814083836284	13.864949766002068	19.389969947260514	39.746939448374576
10	17.62886894676727	25.83351541167805	32.08702341810989	24.4505922234448
11	37.16099083764325	21.20190024374512	20.88881271399657	20.748296204615063
12	22.047288907961686	24.996761840216767	28.74223332282294	24.213715928998607
13	30.547752717110438	19.06262143099187	24.631536667089698	25.758089184807993
14	23.325217218374956	20.097524214239268	24.310468218279443	32.26679034910633
15	25.724922578543364	27.475491382569935	21.41588045891593	25.383705579970773
16	25.968406027294744	25.500410166905873	23.35216917455055	25.17901463124883
17	24.370259693872878	25.947734138577538	24.65561025901353	25.026395908536053
18	24.87161841263445	24.23242529663506	25.878326309688443	25.017629981042045
19	24.995453492829604	24.72822353900118	25.461159745290928	24.815163222878287
20	25.106139681783752	24.313739086747358	25.4124892224884	25.167632008980494
21	26.028982511320475	24.188399407056966	25.216891288107252	24.565726793515307
22	25.686130078513926	24.263171460233433	25.34314681096866	24.707551650283975
23	24.711215022968037	24.319299563142806	25.63732872097268	25.332156692916474
24	24.76590394375153	25.027050082229636	25.28885039440132	24.918195579617517
25	25.45998223264248	24.299216430749826	25.287214960167365	24.953586376440327
26	25.110784315008182	25.155398960910507	25.22336760767372	24.510449116407592
27	25.6283665413706	24.264283555512524	25.14388550390346	24.96346439921342
28	24.644096802006484	24.540148602096234	25.95211710232454	24.863637493572742
29	24.40074418799382	24.656591519553906	25.868448286915346	25.074216005536925
30	24.004969103376453	25.474177801793218	25.964153898286458	24.556699196543867
31	25.095084146362208	24.984136287930625	25.018349572104988	24.902429993602183
32	24.57632440735134	24.69322524639452	25.168417017412796	25.562033328841338
33	24.219668909610203	24.506393239507382	25.78785408786599	25.48608376301642
34	25.327512059692044	24.3299625943482	25.38972397795173	24.95280136800803
35	24.954436802241982	24.397277067417832	26.365227789821844	24.28305834051834
36	24.410229706550766	24.927550263435748	25.5776026627486	25.08461736726489
37	24.81463988392342	24.28004914152786	25.97821863269848	24.927092341850237
38	24.12193535978899	24.22503313389758	26.403889455112562	25.249142051200867
39	24.624340756460292	24.042518673388084	26.147911288813763	25.18522928133786
40	24.286198374247537	24.303795646604904	26.67432486003954	24.735681119108023
41	23.679256021341764	24.295945562281915	27.17228187559448	24.852516540781842
42	24.250872994794086	24.230855279770463	27.008869286937593	24.509402438497858
43	23.675788900765777	23.587279200024074	27.09476229290496	25.64216960630519
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	172.0
1	199.0
2	226.0
3	477.0
4	728.0
5	728.0
6	956.5
7	1185.0
8	1064.5
9	944.0
10	1328.5
11	1713.0
12	1713.0
13	3104.5
14	4496.0
15	6435.0
16	8374.0
17	8200.0
18	8026.0
19	8026.0
20	9545.5
21	11065.0
22	10577.0
23	10089.0
24	11859.5
25	13630.0
26	13630.0
27	16967.5
28	20305.0
29	23698.5
30	27092.0
31	31051.0
32	35010.0
33	35010.0
34	41021.5
35	47033.0
36	52920.0
37	58807.0
38	65803.0
39	72799.0
40	72799.0
41	79294.5
42	85790.0
43	89239.0
44	92688.0
45	97633.0
46	102578.0
47	102578.0
48	107879.0
49	113180.0
50	113101.0
51	113022.0
52	115262.0
53	117502.0
54	117502.0
55	114338.5
56	111175.0
57	106456.0
58	101737.0
59	98024.0
60	94311.0
61	94311.0
62	87506.0
63	80701.0
64	72502.5
65	64304.0
66	56159.5
67	48015.0
68	48015.0
69	40157.5
70	32300.0
71	27096.0
72	21892.0
73	16926.5
74	11961.0
75	11961.0
76	9117.5
77	6274.0
78	4942.0
79	3610.0
80	3075.0
81	2540.0
82	2540.0
83	1861.5
84	1183.0
85	1027.5
86	872.0
87	787.5
88	703.0
89	703.0
90	599.5
91	496.0
92	288.5
93	81.0
94	55.5
95	30.0
96	30.0
97	17.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1528646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.7125781192543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85204512098642	42.619197445414706
2	10.705448116726357	11.286235403091242
3	3.282748917328292	5.191264762516954
4	1.473715170252991	3.1073330414996376
5	0.8139552305041134	2.1452839336761858
6	0.5416634041293168	1.7131484702704698
7	0.3756219056588943	1.3859999331743347
8	0.2603484144401384	1.0978908907519833
9	0.21359025082138772	1.0133003503740168
>10	1.2708273844627547	12.729594974776848
>50	0.11449498006919889	4.208183775462794
>100	0.08672008516302784	9.23314590268518
>500	0.0068331840865295725	2.494905308117297
>1k	0.0019878353706267848	1.774515808188426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3686	0.24112842345448193	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3665	0.23975465869795887	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2842	0.18591616371612527	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1747	0.11428414426884968	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1599	0.10460237360382979	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1594	0.10427528675703858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	6.541736935824252E-5	0.0
12	0.0	0.0	0.0	1.3083473871648504E-4	0.0
13	0.0	0.0	0.0	1.3083473871648504E-4	0.0
14	0.0	0.0	0.0	1.9625210807472756E-4	0.0
15	0.0	0.0	0.0	3.270868467912126E-4	0.0
16	0.0	0.0	0.0	7.195910629406678E-4	0.0
17	0.0	0.0	0.0	7.850084322989102E-4	0.0
18	0.0	0.0	0.0	8.504258016571528E-4	0.0
19	0.0	0.0	0.0	0.001112095279090123	0.0
20	0.0	0.0	0.0	0.0013083473871648504	0.0
21	0.0	0.0	0.0	0.0014391821258813355	0.0
22	0.0	0.0	0.0	0.0018316863420307907	0.0
23	0.0	0.0	0.0	0.0022896079275384882	0.0
24	0.0	0.0	0.0	0.003990459530852794	0.0
25	0.0	0.0	0.0	0.005037137440584674	0.0
26	0.0	0.0	0.0	0.00804633643106383	0.0
27	0.0	0.0	0.0	0.018578532897740876	0.0
28	0.0	0.0	0.0	0.04337171588451479	0.0
29	0.0	0.0	0.0	0.07594956582491957	0.0
30	0.0	0.0	0.0	0.12841429605023008	0.0
31	0.0	0.0	0.0	0.28495806092450443	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATTC	130	3.274181E-11	19.923077	3
GGTATCA	1520	0.0	19.473684	1
GATCGGA	70	1.2195312E-4	18.5	17
TACGGGT	60	9.2387194E-4	18.5	12
GTAATAC	145	1.546141E-10	17.862068	3
ACAACGC	65	0.0015802562	17.076923	8
AAGACGG	390	0.0	17.076923	5
GTTCTAG	255	0.0	15.960784	1
GTATTAA	190	2.7284841E-11	15.578948	1
GACGGAC	400	0.0	15.262499	7
TACGCAG	85	5.365589E-4	15.235294	5
CTAGACT	110	1.4524128E-5	15.136364	4
CTAGCGG	325	0.0	14.800001	29
AGACGGA	440	0.0	14.715909	6
CTAGAAC	265	0.0	14.6603775	3
CGCAGTT	115	2.2106775E-5	14.47826	27
TCTATAC	115	2.2106775E-5	14.47826	3
GTCCTAA	205	9.276846E-11	14.439025	1
ACGGACC	440	0.0	14.295454	8
GTCTAGG	130	4.445981E-6	14.230769	1
>>END_MODULE