FastQCFastQC Report
Fri 10 Feb 2017
ERR1630139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161311
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63110.5434375460148057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42270.36398518570822114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40720.3506382011364742No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG30600.26349530831964907No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT21800.18771887978327942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.16886088222706924No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC18050.15542778807744007No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA17390.14974455593721234No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG16830.14492241957580698No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC14720.12675329864265472No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA14250.12270614848218953No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA14080.12124228565819146No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC13650.11753957380925523No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC12900.11108135546808737No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA12380.10660365741821097No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT12330.10617310952879977No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT12130.10445091797115502No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC11940.10281483599139249No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT11630.1001454390770431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTT400.001931199723.12512
ACGCAAT855.1808456E-821.76470611
TAGACTA450.003825832420.5555555
TCGCCAT3800.019.47368413
GTCGCCA3900.018.9743612
GCCGAAT609.236895E-418.522
AACAACG500.00703492618.4999987
GACGCAA1002.8749855E-718.49999810
AGGTCGC4350.017.8620710
CGGCCTT5100.017.7745124
TCTAGCG1156.4050255E-817.69565228
CTTATAC4500.017.67777837
TCGGCCT5150.017.2427223
GAGGTCG4550.017.0769239
GGCGCAT650.001579947417.07692316
TCTATAC1201.0411895E-716.9583323
ATCTCGC5350.016.59813111
GCTTAGG8400.016.5178571
GACGGAC2700.016.4444437
TAGGACA8750.016.284
Produced by FastQC (version 0.11.5)