##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1161311
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87037580803075	33.0	31.0	34.0	30.0	34.0
2	32.07489811084197	33.0	31.0	34.0	30.0	34.0
3	32.19393685240215	34.0	31.0	34.0	30.0	34.0
4	35.84201217417212	37.0	35.0	37.0	35.0	37.0
5	35.76910750005813	37.0	35.0	37.0	35.0	37.0
6	35.82463095587659	37.0	35.0	37.0	35.0	37.0
7	35.80299592443368	37.0	35.0	37.0	35.0	37.0
8	35.80903048365167	37.0	35.0	37.0	35.0	37.0
9	37.548153767595416	39.0	37.0	39.0	35.0	39.0
10	37.44330588447022	39.0	37.0	39.0	34.0	39.0
11	37.508200645649616	39.0	37.0	39.0	35.0	39.0
12	37.44172060714141	39.0	37.0	39.0	35.0	39.0
13	37.44367529455934	39.0	37.0	39.0	35.0	39.0
14	38.671212965346925	40.0	38.0	41.0	34.0	41.0
15	38.68871129266837	40.0	38.0	41.0	35.0	41.0
16	38.640733619159725	40.0	38.0	41.0	34.0	41.0
17	38.63259368076252	40.0	38.0	41.0	34.0	41.0
18	38.586866911619715	40.0	38.0	41.0	34.0	41.0
19	38.631847110722276	40.0	38.0	41.0	34.0	41.0
20	38.61315788793872	40.0	38.0	41.0	34.0	41.0
21	38.55422621502767	40.0	38.0	41.0	34.0	41.0
22	38.52861980985283	40.0	38.0	41.0	34.0	41.0
23	38.46831555027034	40.0	38.0	41.0	34.0	41.0
24	38.434558873548944	40.0	38.0	41.0	34.0	41.0
25	38.413758243915716	40.0	38.0	41.0	34.0	41.0
26	38.24984263474642	40.0	38.0	41.0	34.0	41.0
27	38.13993667501642	40.0	38.0	41.0	33.0	41.0
28	38.068328811145335	40.0	38.0	41.0	33.0	41.0
29	38.011102107876354	40.0	37.0	41.0	33.0	41.0
30	37.93450505506277	40.0	37.0	41.0	33.0	41.0
31	37.85912559168044	40.0	37.0	41.0	33.0	41.0
32	37.75045530439305	40.0	37.0	41.0	33.0	41.0
33	37.664088258872944	40.0	37.0	41.0	33.0	41.0
34	37.6096291174371	40.0	37.0	41.0	33.0	41.0
35	37.46663985788475	40.0	37.0	41.0	32.0	41.0
36	37.372930248658626	40.0	36.0	41.0	32.0	41.0
37	37.3130341484753	40.0	36.0	41.0	31.0	41.0
38	37.19165494858828	40.0	36.0	41.0	31.0	41.0
39	37.10437514154262	39.0	36.0	41.0	31.0	41.0
40	37.001720469366084	39.0	36.0	41.0	31.0	41.0
41	36.86711656050791	39.0	35.0	41.0	30.0	41.0
42	36.81952896338707	39.0	35.0	41.0	30.0	41.0
43	35.599098777157884	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	5.0
13	4.0
14	3.0
15	5.0
16	6.0
17	25.0
18	50.0
19	78.0
20	216.0
21	377.0
22	661.0
23	1158.0
24	1885.0
25	2808.0
26	4456.0
27	6552.0
28	9404.0
29	13099.0
30	17511.0
31	23277.0
32	30250.0
33	38319.0
34	50947.0
35	67704.0
36	95800.0
37	148174.0
38	267534.0
39	380995.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.760896951807055	19.02332794574408	12.786325110155678	25.42944999229319
2	17.131328300515538	22.022955091271847	35.74615240878627	25.09956419942634
3	18.94625987353947	23.75074377147896	31.649403131460907	25.65359322352066
4	14.091574091694644	15.813593430183644	35.30570191791863	34.78913056020308
5	13.405280755973207	38.111754732367125	34.722567856500106	13.760396655159557
6	33.14064880122552	36.120384634262486	16.474312221274058	14.264654343237945
7	28.113657754038325	32.133252849581204	21.492433981939378	18.260655414441093
8	27.024543812983776	35.05899797728602	20.26907520896642	17.647383000763792
9	26.495142128163774	14.773045291054679	19.622220059915044	39.10959252086651
10	16.318367775729325	28.17961769069612	32.935363567554255	22.566650966020298
11	34.659191207178786	22.6413079700442	22.02683002227655	20.67267080050047
12	20.643824091909917	26.71041607286937	29.657860814200504	22.98789902102021
13	29.116059350165457	19.800466886131275	26.534149766944427	24.549323996758833
14	22.245203911785904	21.218863853007505	25.680803850131444	30.855128385075144
15	24.367202239537903	28.38628067761349	23.34344546809597	23.903071614752637
16	24.164241964469465	27.534743061936034	24.260340253386044	24.040674720208454
17	23.12601878394332	27.81451308047543	25.822712434481375	23.236755701099877
18	22.151775019783678	26.124354285802852	27.79462176798463	23.929248926428837
19	23.74506053933873	26.376310910686286	27.839743186794923	22.03888536318006
20	23.948020814407165	25.320693595427922	27.842929241176567	22.888356348988342
21	23.507053666072224	26.65961142191885	26.307337138802612	23.525997773206317
22	23.601429763431156	26.065197005797756	26.654444847245912	23.67892838352517
23	22.639499668908673	26.489803334335075	27.036082496419994	23.834614500336258
24	23.758235304754713	26.58882934889965	26.999916473709458	22.653018872636185
25	23.836508911049666	25.597621997897203	27.036943592198813	23.52892549885431
26	23.55260563277193	26.542416286421123	26.830883372326618	23.07409470848033
27	23.875344330674555	25.819526380099735	26.486789499109197	23.818339790116518
28	22.661027063379233	26.422723973164814	27.522084953987346	23.39416400946861
29	22.906267141187847	26.96349212226527	27.06406810923172	23.06617262731516
30	22.313661026202283	27.830960009850937	27.022735511848246	22.832643452098534
31	23.374014368244165	26.698705170277385	26.89804884307477	23.029231618403685
32	22.403473315933457	26.83734159066779	26.972791956676552	23.786393136722204
33	22.58473397737557	26.33446165583552	27.34917692159981	23.7316274451891
34	23.1353186183546	26.4630232556137	27.065531972055716	23.336126153975982
35	23.673331260962826	26.391810634705088	27.13596960676339	22.798888497568694
36	22.661543720846524	27.02368271720495	27.099114707429795	23.21565885451873
37	23.993400561951105	25.49739044924228	26.84664142507907	23.662567563727546
38	23.05213676612036	26.161381404292218	27.77653875662936	23.009943072958063
39	23.245883316355396	25.857500703945803	27.55911207247671	23.337503907222096
40	22.845043231313575	25.412916953339803	28.324798439005573	23.41724137634105
41	21.93848159536937	26.233627340135417	28.730202331675148	23.097688732820064
42	23.126191003099084	25.229934100340046	28.22103639765747	23.422838498903396
43	22.197499205639144	25.15011052164321	28.515875592326257	24.136514680391386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	182.5
2	179.0
3	458.5
4	738.0
5	738.0
6	1047.5
7	1357.0
8	1276.0
9	1195.0
10	1748.0
11	2301.0
12	2301.0
13	3963.5
14	5626.0
15	9743.5
16	13861.0
17	12632.5
18	11404.0
19	11404.0
20	12498.5
21	13593.0
22	13974.5
23	14356.0
24	15870.0
25	17384.0
26	17384.0
27	19238.5
28	21093.0
29	27207.5
30	33322.0
31	35301.5
32	37281.0
33	37281.0
34	40811.0
35	44341.0
36	47575.5
37	50810.0
38	58254.0
39	65698.0
40	65698.0
41	71038.5
42	76379.0
43	71549.5
44	66720.0
45	72625.0
46	78530.0
47	78530.0
48	82383.0
49	86236.0
50	88275.0
51	90314.0
52	95450.5
53	100587.0
54	100587.0
55	87099.0
56	73611.0
57	68315.0
58	63019.0
59	55829.0
60	48639.0
61	48639.0
62	45091.5
63	41544.0
64	36756.5
65	31969.0
66	27832.0
67	23695.0
68	23695.0
69	19944.5
70	16194.0
71	14157.5
72	12121.0
73	9747.0
74	7373.0
75	7373.0
76	5574.0
77	3775.0
78	3165.5
79	2556.0
80	1964.0
81	1372.0
82	1372.0
83	1005.0
84	638.0
85	569.0
86	500.0
87	474.0
88	448.0
89	448.0
90	376.5
91	305.0
92	173.0
93	41.0
94	28.5
95	16.0
96	16.0
97	9.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1161311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.215700461589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.86909478819315	38.760689627157504
2	9.207941356192293	8.511029191713213
3	2.5051718931117994	3.4733482145054033
4	1.0976253764151327	2.029101024617626
5	0.6191300696636957	1.4306764923170048
6	0.4155622441319007	1.1523300118767388
7	0.28381999651634227	0.9181857960805956
8	0.2123323159295402	0.785046936905209
9	0.17156476282610547	0.7136087119681366
>10	1.243555670933857	11.626969038838709
>50	0.18308246482882468	5.9891714575252974
>100	0.17165149787579803	16.19576560138043
>500	0.014600672536136677	4.296014786949301
>1k	0.004679702735941242	3.572096630377647
>5k	1.871881094376497E-4	0.5459664777872497
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6311	0.5434375460148057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4227	0.36398518570822114	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4072	0.3506382011364742	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3060	0.26349530831964907	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2180	0.18771887978327942	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1961	0.16886088222706924	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1805	0.15542778807744007	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1739	0.14974455593721234	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1683	0.14492241957580698	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1472	0.12675329864265472	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1425	0.12270614848218953	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1408	0.12124228565819146	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1365	0.11753957380925523	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1290	0.11108135546808737	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1238	0.10660365741821097	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1233	0.10617310952879977	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1213	0.10445091797115502	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1194	0.10281483599139249	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1163	0.1001454390770431	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	8.610957788223826E-5	0.0	0.0	0.0
2	0.0	8.610957788223826E-5	0.0	0.0	0.0
3	0.0	8.610957788223826E-5	0.0	0.0	0.0
4	0.0	8.610957788223826E-5	0.0	0.0	0.0
5	0.0	8.610957788223826E-5	0.0	0.0	0.0
6	0.0	8.610957788223826E-5	0.0	0.0	0.0
7	0.0	8.610957788223826E-5	0.0	0.0	0.0
8	0.0	8.610957788223826E-5	0.0	0.0	0.0
9	0.0	8.610957788223826E-5	0.0	0.0	0.0
10	0.0	8.610957788223826E-5	0.0	8.610957788223826E-5	0.0
11	0.0	8.610957788223826E-5	0.0	8.610957788223826E-5	0.0
12	0.0	8.610957788223826E-5	0.0	2.583287336467148E-4	0.0
13	0.0	8.610957788223826E-5	0.0	2.583287336467148E-4	0.0
14	0.0	8.610957788223826E-5	0.0	3.4443831152895305E-4	0.0
15	0.0	8.610957788223826E-5	0.0	4.305478894111913E-4	0.0
16	0.0	8.610957788223826E-5	0.0	6.888766230579061E-4	0.0
17	0.0	8.610957788223826E-5	0.0	0.001033314934586859	0.0
18	0.0	8.610957788223826E-5	0.0	0.001291643668233574	0.0
19	0.0	8.610957788223826E-5	0.0	0.001636081979762527	0.0
20	0.0	8.610957788223826E-5	0.0	0.0017221915576447653	0.0
21	0.0	8.610957788223826E-5	0.0	0.00198052029129148	0.0
22	0.0	8.610957788223826E-5	0.0	0.0024110681807026713	0.0
23	0.0	8.610957788223826E-5	0.0	0.003616602271054007	0.0
24	0.0	8.610957788223826E-5	0.0	0.004391588471994151	0.0
25	0.0	8.610957788223826E-5	0.0	0.0050804650950520575	0.0
26	0.0	8.610957788223826E-5	0.0	0.006285999185403394	0.0
27	0.0	8.610957788223826E-5	0.0	0.014466409084216028	0.0
28	0.0	8.610957788223826E-5	0.0	0.04012706329312303	0.0
29	0.0	8.610957788223826E-5	0.0	0.08309574265635992	0.0
30	0.0	8.610957788223826E-5	0.0	0.13803365334522794	0.0
31	0.0	8.610957788223826E-5	0.0	0.2975085915831332	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTT	40	0.0019311997	23.125	12
ACGCAAT	85	5.1808456E-8	21.764706	11
TAGACTA	45	0.0038258324	20.555555	5
TCGCCAT	380	0.0	19.473684	13
GTCGCCA	390	0.0	18.97436	12
GCCGAAT	60	9.236895E-4	18.5	22
AACAACG	50	0.007034926	18.499998	7
GACGCAA	100	2.8749855E-7	18.499998	10
AGGTCGC	435	0.0	17.86207	10
CGGCCTT	510	0.0	17.77451	24
TCTAGCG	115	6.4050255E-8	17.695652	28
CTTATAC	450	0.0	17.677778	37
TCGGCCT	515	0.0	17.24272	23
GAGGTCG	455	0.0	17.076923	9
GGCGCAT	65	0.0015799474	17.076923	16
TCTATAC	120	1.0411895E-7	16.958332	3
ATCTCGC	535	0.0	16.598131	11
GCTTAGG	840	0.0	16.517857	1
GACGGAC	270	0.0	16.444443	7
TAGGACA	875	0.0	16.28	4
>>END_MODULE