FastQCFastQC Report
Fri 10 Feb 2017
ERR1630138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645448
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20900.3238061005689072No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC15210.23565027701689367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15090.23379110323372296No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT13250.20528377189177127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12900.1998611816908566No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA10340.1601988076498804No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA9410.14579021083030702No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG8990.13928310258920937No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG8250.12781819759298968No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG7360.11402932536780655No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG7170.11108563354445283No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA6720.1041137318575625No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG6540.10132497118280637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT1550.023.87096611
GCAGTCG1650.023.5454549
CAGTCGG1800.021.58333210
TCTAGCG450.00382401820.55555528
GTCGGTG1800.020.55555512
GCTTTAT2800.020.4821431
CCGGTCC555.1397353E-420.1818189
TATTGAG2800.019.821435
TCGGTGA1900.019.47368413
GGTATCA7550.019.3576151
CCGCTTA609.230835E-418.525
TAAGACT902.1492415E-618.54
TAATAGT1054.789199E-717.619054
TTATACT1054.789199E-717.619054
ACGCTGA1054.789199E-717.6190511
CGGTGAT2000.017.57499914
CGCGGTT650.00157891917.07692310
TCCGATA650.00157891917.0769238
TTAGACT650.00157891917.0769234
TTTATTG3600.016.9583343
Produced by FastQC (version 0.11.5)