##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630138.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645448
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.452990790892528	31.0	31.0	34.0	30.0	34.0
2	31.676277562251336	31.0	31.0	34.0	30.0	34.0
3	31.801263618447962	31.0	31.0	34.0	30.0	34.0
4	35.52026654354805	37.0	35.0	37.0	33.0	37.0
5	35.406671025396314	37.0	35.0	37.0	33.0	37.0
6	35.46632261622935	37.0	35.0	37.0	33.0	37.0
7	35.46490189759671	37.0	35.0	37.0	33.0	37.0
8	35.4621518697091	37.0	35.0	37.0	33.0	37.0
9	37.13883070363531	39.0	37.0	39.0	33.0	39.0
10	36.981318402102104	39.0	37.0	39.0	33.0	39.0
11	37.063300219382505	39.0	37.0	39.0	33.0	39.0
12	36.96962574831745	39.0	37.0	39.0	33.0	39.0
13	36.99188470643646	39.0	37.0	39.0	33.0	39.0
14	38.11402932536781	40.0	37.0	41.0	33.0	41.0
15	38.11795063273881	40.0	37.0	41.0	33.0	41.0
16	38.06819759298968	40.0	37.0	41.0	33.0	41.0
17	38.04476115814132	40.0	37.0	41.0	33.0	41.0
18	37.99115033279211	40.0	37.0	41.0	33.0	41.0
19	38.00333566762931	40.0	37.0	41.0	33.0	41.0
20	37.99263302388419	40.0	37.0	41.0	33.0	41.0
21	37.9153998463083	40.0	37.0	41.0	33.0	41.0
22	37.88247697723132	40.0	37.0	41.0	33.0	41.0
23	37.84757563738675	40.0	37.0	41.0	33.0	41.0
24	37.78756615560045	40.0	37.0	41.0	33.0	41.0
25	37.78245032907376	40.0	37.0	41.0	33.0	41.0
26	37.60131102737943	39.0	37.0	41.0	32.0	41.0
27	37.46940729539793	39.0	37.0	41.0	32.0	41.0
28	37.392933280450166	39.0	37.0	41.0	32.0	41.0
29	37.329126436211745	39.0	36.0	40.0	31.0	41.0
30	37.26362929314213	39.0	36.0	40.0	31.0	41.0
31	37.19048939651219	39.0	36.0	40.0	31.0	41.0
32	37.12031178344344	39.0	36.0	40.0	31.0	41.0
33	37.011370396995574	39.0	36.0	40.0	31.0	41.0
34	36.95641321996505	39.0	36.0	40.0	31.0	41.0
35	36.816820255078646	39.0	36.0	40.0	30.0	41.0
36	36.726998921679204	39.0	35.0	40.0	30.0	41.0
37	36.658564284032174	39.0	35.0	40.0	30.0	41.0
38	36.55368209367757	39.0	35.0	40.0	30.0	41.0
39	36.460518895402885	39.0	35.0	40.0	30.0	41.0
40	36.348533111885075	39.0	35.0	40.0	30.0	41.0
41	36.244214870911364	38.0	35.0	40.0	30.0	41.0
42	36.189316567717306	38.0	35.0	40.0	29.0	41.0
43	34.91701732749966	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	1.0
15	1.0
16	4.0
17	13.0
18	26.0
19	80.0
20	156.0
21	242.0
22	536.0
23	872.0
24	1393.0
25	2348.0
26	3427.0
27	4984.0
28	7023.0
29	9557.0
30	12739.0
31	16309.0
32	20737.0
33	26507.0
34	34691.0
35	46337.0
36	64812.0
37	99522.0
38	171548.0
39	121579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.28481922633582	18.985417880293998	12.137925905727496	25.59183698764269
2	18.815303479133874	21.111228170201162	33.05161686146676	27.021851489198202
3	20.24361373805481	22.73970947311015	29.816499547601044	27.200177241233998
4	14.589246538838141	15.146069086897782	35.28216060782588	34.98252376643819
5	14.405033403155636	36.892514966348955	34.62897088533855	14.073480745156852
6	35.12552521659374	35.91071627768619	15.103153158736257	13.860605346983801
7	30.217461360171537	30.541267460740446	20.58694116334701	18.654330015741007
8	29.54800386708147	32.164790966894316	19.360816053345893	18.926389112678326
9	27.361925360369852	13.644941188135991	18.63666786480088	40.356465586693275
10	16.875100705246588	26.957709993678808	33.013658730060364	23.15353057101424
11	37.05100953136426	21.586711865247086	20.75147804315762	20.61080056023103
12	23.116347095350825	25.20404432270299	28.515387761678713	23.164220820267474
13	31.704025730965157	18.895867676404606	24.383219097433102	25.016887495197132
14	23.017191160248384	19.662776861962545	24.998295757365426	32.32173622042364
15	25.55372392508769	27.824394838933582	21.597247183351715	25.024634052627015
16	24.93523877988622	26.319083799159653	23.439223609028147	25.306453811925977
17	24.231541502956087	26.144476394690198	24.76977231318402	24.854209789169694
18	24.053990406663278	25.15508607974616	26.24750560850758	24.543417905082983
19	25.449300330932935	24.86753386794908	26.329464186115693	23.353701615002294
20	26.182434526096603	24.46641712422999	25.076845849704394	24.274302499969014
21	25.650091099515375	24.527924790223224	25.26307309031866	24.558911019942737
22	25.550470370967144	24.753659473729876	25.694711270311473	24.00115888499151
23	25.0890854104436	24.641799184442434	25.06956408572031	25.19955131939366
24	24.391740310605968	25.14284651900695	26.18956135893209	24.27585181145499
25	25.00588738364671	25.116043430299573	25.322876513677322	24.555192672376396
26	24.962196799742195	25.275622513355067	25.270354854302745	24.491825832599993
27	24.958323521027257	24.369585156356514	25.535132187255982	25.13695913536025
28	24.572544961019325	25.228368513032805	25.40452522898824	24.794561296959632
29	24.633897695863958	25.410412612634946	26.060503712150318	23.895185979350778
30	24.923464012592802	25.486638737744947	24.893097507467683	24.69679974219457
31	24.297852034555845	25.94399548840495	25.690528129299338	24.067624347739862
32	24.12448407927517	25.377257346835062	25.464638514644093	25.03362005924567
33	23.745832352102724	25.820515362972696	25.769852877381293	24.663799407543287
34	25.454413058836657	24.387867031891027	25.591682056494093	24.566037852778226
35	24.571770305276335	24.943605061910485	25.584865085955798	24.89975954685738
36	23.854439087269615	25.894417520853736	25.845459277896904	24.40568411397975
37	24.807575513441826	24.835463120189388	26.10527881409502	24.25168255227377
38	24.995971790136462	25.34208797610342	25.37896158946964	24.282978644290477
39	24.654348607478838	25.114184256516403	25.769852877381293	24.461614258623467
40	23.783015827766143	24.473234094768284	26.394535268526663	25.349214808938907
41	23.03965617679503	24.991943580272928	26.74979239226088	25.21860785067116
42	23.792466627830592	24.67061637808158	27.07793656499052	24.45898042909731
43	24.111624793941573	24.44302252079176	26.709200431328316	24.73615225393835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	27.5
2	31.0
3	103.5
4	176.0
5	176.0
6	266.5
7	357.0
8	325.0
9	293.0
10	427.5
11	562.0
12	562.0
13	1085.5
14	1609.0
15	2797.0
16	3985.0
17	3722.0
18	3459.0
19	3459.0
20	4349.0
21	5239.0
22	5359.5
23	5480.0
24	6629.0
25	7778.0
26	7778.0
27	9329.5
28	10881.0
29	12560.0
30	14239.0
31	16326.0
32	18413.0
33	18413.0
34	20537.5
35	22662.0
36	25349.0
37	28036.0
38	29531.5
39	31027.0
40	31027.0
41	32695.5
42	34364.0
43	35412.0
44	36460.0
45	38367.0
46	40274.0
47	40274.0
48	42861.5
49	45449.0
50	45344.5
51	45240.0
52	45919.0
53	46598.0
54	46598.0
55	44509.5
56	42421.0
57	41874.5
58	41328.0
59	40877.5
60	40427.0
61	40427.0
62	37090.5
63	33754.0
64	31096.5
65	28439.0
66	24844.0
67	21249.0
68	21249.0
69	18578.0
70	15907.0
71	13106.0
72	10305.0
73	7268.5
74	4232.0
75	4232.0
76	3200.5
77	2169.0
78	1698.0
79	1227.0
80	921.0
81	615.0
82	615.0
83	489.5
84	364.0
85	277.0
86	190.0
87	143.5
88	97.0
89	97.0
90	81.5
91	66.0
92	40.5
93	15.0
94	10.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	645448.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.20387338941705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61437956821628	59.55229544984153
2	7.2117737218991	9.693182562392641
3	1.6335145897701762	3.2933552301204134
4	0.6642901973435127	1.785714972644172
5	0.3742218443446118	1.2574578723444716
6	0.25963076613246805	1.0468915881098237
7	0.18953295659303687	0.8916144172600229
8	0.1339384768526556	0.7200947552301787
9	0.10661815648563192	0.6448637780526116
>10	0.6951344359492689	8.93941413061117
>50	0.0609050647907996	2.8361455447456057
>100	0.05190761261345003	6.670891978624245
>500	0.002768406006019484	1.3085348339238805
>1k	0.001384203003009742	1.3595428860993495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2090	0.3238061005689072	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1521	0.23565027701689367	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1509	0.23379110323372296	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1325	0.20528377189177127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1290	0.1998611816908566	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1034	0.1601988076498804	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	941	0.14579021083030702	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	899	0.13928310258920937	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	825	0.12781819759298968	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	736	0.11402932536780655	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	717	0.11108563354445283	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	672	0.1041137318575625	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	654	0.10132497118280637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	1.5493114859756323E-4	0.0	0.0	0.0	0.0
6	1.5493114859756323E-4	0.0	0.0	0.0	0.0
7	1.5493114859756323E-4	0.0	0.0	0.0	0.0
8	1.5493114859756323E-4	0.0	0.0	0.0	0.0
9	1.5493114859756323E-4	0.0	0.0	0.0	0.0
10	1.5493114859756323E-4	0.0	0.0	0.0	0.0
11	1.5493114859756323E-4	0.0	0.0	1.5493114859756323E-4	0.0
12	1.5493114859756323E-4	0.0	0.0	1.5493114859756323E-4	0.0
13	1.5493114859756323E-4	0.0	0.0	1.5493114859756323E-4	0.0
14	1.5493114859756323E-4	0.0	0.0	1.5493114859756323E-4	0.0
15	1.5493114859756323E-4	0.0	0.0	1.5493114859756323E-4	0.0
16	1.5493114859756323E-4	0.0	0.0	4.6479344579268973E-4	0.0
17	1.5493114859756323E-4	0.0	0.0	6.197245943902529E-4	0.0
18	3.0986229719512647E-4	0.0	0.0	7.746557429878162E-4	0.0
19	3.0986229719512647E-4	0.0	0.0	9.295868915853795E-4	0.0
20	3.0986229719512647E-4	0.0	0.0	0.0010845180401829427	0.0
21	3.0986229719512647E-4	0.0	0.0	0.001859173783170759	0.0
22	4.6479344579268973E-4	0.0	0.0	0.003253554120548828	0.0
23	4.6479344579268973E-4	0.0	0.0	0.004183141012134207	0.0
24	4.6479344579268973E-4	0.0	0.0	0.006042314795304967	0.0
25	4.6479344579268973E-4	0.0	0.0	0.006816970538292783	0.0
26	4.6479344579268973E-4	0.0	0.0	0.007746557429878162	0.0
27	4.6479344579268973E-4	0.0	0.0	0.012549423036402623	0.0
28	4.6479344579268973E-4	0.0	0.0	0.02339460343823205	0.0
29	4.6479344579268973E-4	0.0	0.0	0.0438455150531104	0.0
30	4.6479344579268973E-4	0.0	0.0	0.07436695132683036	0.0
31	4.6479344579268973E-4	0.0	0.0	0.14486062393872162	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	155	0.0	23.870966	11
GCAGTCG	165	0.0	23.545454	9
CAGTCGG	180	0.0	21.583332	10
TCTAGCG	45	0.003824018	20.555555	28
GTCGGTG	180	0.0	20.555555	12
GCTTTAT	280	0.0	20.482143	1
CCGGTCC	55	5.1397353E-4	20.181818	9
TATTGAG	280	0.0	19.82143	5
TCGGTGA	190	0.0	19.473684	13
GGTATCA	755	0.0	19.357615	1
CCGCTTA	60	9.230835E-4	18.5	25
TAAGACT	90	2.1492415E-6	18.5	4
TAATAGT	105	4.789199E-7	17.61905	4
TTATACT	105	4.789199E-7	17.61905	4
ACGCTGA	105	4.789199E-7	17.61905	11
CGGTGAT	200	0.0	17.574999	14
CGCGGTT	65	0.001578919	17.076923	10
TCCGATA	65	0.001578919	17.076923	8
TTAGACT	65	0.001578919	17.076923	4
TTTATTG	360	0.0	16.958334	3
>>END_MODULE