FastQCFastQC Report
Fri 10 Feb 2017
ERR1630137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519056
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC17610.3392697512407139No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT9330.17974939120249067No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG7210.1389060139946364No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT5950.1146311765975155No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG5650.1088514534077248No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG5480.10557627693351006No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5480.10557627693351006No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT5460.10519096205419069No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5370.10345704509725348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAT200.001841006537.03
CAAGGGT358.8628184E-426.428574
CGCAAGA809.749783E-1025.43752
GGTATCA2400.023.8958341
ACGTTCG701.9187064E-723.7857137
ATTAGAC551.9000532E-523.5454543
AAGACGG952.8012437E-1023.3684225
TATTTCG502.6993363E-422.227
TTAGACT502.6993363E-422.24
CAGACGT705.093034E-621.1428574
TTCTATA450.00382302420.5555552
TTATATA450.00382302420.5555554
GCTTAGA450.00382302420.5555551
GCATCAG4550.020.3296715
TGCATCA4750.020.25263414
TATTAGA555.137872E-420.1818182
CAAGACG1101.7480488E-920.1818184
CAGGACA5250.020.0857144
CCAGGAC5300.019.8962273
CGTGTTG759.2497285E-619.73333234
Produced by FastQC (version 0.11.5)