##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630137.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519056
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77518803366111	31.0	31.0	34.0	30.0	34.0
2	31.993339832310966	33.0	31.0	34.0	30.0	34.0
3	32.112700749052124	34.0	31.0	34.0	30.0	34.0
4	35.77024444375944	37.0	35.0	37.0	35.0	37.0
5	35.70298965814864	37.0	35.0	37.0	33.0	37.0
6	35.75412094263432	37.0	35.0	37.0	35.0	37.0
7	35.73055315804075	37.0	35.0	37.0	33.0	37.0
8	35.72071799574612	37.0	35.0	37.0	33.0	37.0
9	37.4812024136124	39.0	37.0	39.0	35.0	39.0
10	37.356832403440094	39.0	37.0	39.0	34.0	39.0
11	37.43000369902284	39.0	37.0	39.0	34.0	39.0
12	37.34898546592275	39.0	37.0	39.0	34.0	39.0
13	37.34836703554144	39.0	37.0	39.0	34.0	39.0
14	38.56131708332049	40.0	38.0	41.0	34.0	41.0
15	38.575088237107366	40.0	38.0	41.0	34.0	41.0
16	38.531931044049195	40.0	38.0	41.0	34.0	41.0
17	38.48543509756173	40.0	38.0	41.0	34.0	41.0
18	38.387557411917015	40.0	38.0	41.0	34.0	41.0
19	38.407335624672484	40.0	38.0	41.0	34.0	41.0
20	38.380912656823156	40.0	38.0	41.0	34.0	41.0
21	38.32604574458247	40.0	38.0	41.0	34.0	41.0
22	38.28196379581394	40.0	38.0	41.0	34.0	41.0
23	38.211980980857554	40.0	38.0	41.0	33.0	41.0
24	38.16299204710089	40.0	38.0	41.0	33.0	41.0
25	38.12321021238556	40.0	38.0	41.0	33.0	41.0
26	37.9313869794396	40.0	37.0	41.0	33.0	41.0
27	37.81515481951851	40.0	37.0	41.0	33.0	41.0
28	37.70674455164761	40.0	37.0	41.0	32.0	41.0
29	37.64038754970562	40.0	37.0	41.0	32.0	41.0
30	37.57369339724423	40.0	37.0	41.0	32.0	41.0
31	37.53881662094263	40.0	37.0	41.0	32.0	41.0
32	37.460376144385194	40.0	37.0	41.0	31.0	41.0
33	37.35287521962948	40.0	37.0	41.0	31.0	41.0
34	37.26757999136895	40.0	36.0	41.0	31.0	41.0
35	37.11028482475879	39.0	36.0	41.0	31.0	41.0
36	37.021889738294135	39.0	36.0	41.0	30.0	41.0
37	36.95067969544712	39.0	36.0	41.0	30.0	41.0
38	36.835788431306064	39.0	36.0	41.0	30.0	41.0
39	36.766980826731604	39.0	35.0	41.0	30.0	41.0
40	36.64184211337505	39.0	35.0	41.0	30.0	41.0
41	36.509486452328844	39.0	35.0	40.0	30.0	41.0
42	36.478720985789586	39.0	35.0	40.0	30.0	41.0
43	35.09944013748035	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	0.0
15	2.0
16	4.0
17	4.0
18	29.0
19	59.0
20	120.0
21	210.0
22	405.0
23	629.0
24	1053.0
25	1732.0
26	2471.0
27	3604.0
28	4888.0
29	6635.0
30	9028.0
31	11459.0
32	14733.0
33	19188.0
34	25490.0
35	32847.0
36	45361.0
37	66532.0
38	121608.0
39	150962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83468450417681	17.815033445331522	11.705673376283098	27.644608674208566
2	20.461568693936684	19.03551832557566	32.61517061742856	27.887742363059093
3	20.67445516476064	21.17478653555686	28.136270460220093	30.014487839462404
4	15.63183933910792	14.717101815603712	32.32117536450787	37.329883480780495
5	16.100574889799944	35.2622838383527	32.849056749175425	15.788084522671927
6	37.78975678924817	32.82651582873524	14.555462223729233	14.828265158287351
7	31.937401744705774	28.492301408711203	19.797093184550416	19.773203662032614
8	29.18432693196881	31.898870256773836	18.54963626275392	20.367166548503437
9	27.772147899263278	13.28893221540643	17.61833019943898	41.32058968589131
10	18.462940414907063	24.942588082981413	31.269651058845287	25.324820443266237
11	38.91083043062791	20.149656299127646	19.542785364199624	21.39672790604482
12	23.9432739434666	23.187671465121298	27.14331401621405	25.725740575198053
13	31.82219259578928	17.579991368946704	23.15684627477575	27.440969760488272
14	23.946356462501157	18.87561265065812	22.93663882124472	34.241392065596
15	27.530362812490367	25.463533799821214	19.878394624086802	27.127708763601614
16	27.215753213526096	24.013979223821707	21.239712092722172	27.53055546993003
17	25.876398693011932	24.580392096421193	22.947620295305324	26.595588915261555
18	26.101037267655126	22.695624364230447	24.075436947073147	27.127901421041273
19	26.665523565858017	23.126021084430196	23.324843562158996	26.883611787552788
20	26.611386825313648	23.257606115717763	23.218689312906506	26.91231774606208
21	28.489989519435284	22.91852902191671	22.413381215129004	26.178100243519005
22	27.979061989457787	23.10463610862797	22.511636509355444	26.4046653925588
23	26.82774113005148	23.195185105268028	22.69543170679079	27.28164205788971
24	26.83275022348263	23.463556918713973	22.761127893714743	26.942564964088657
25	27.070682161462344	22.90118985234734	23.151644523904935	26.87648346228538
26	27.46678585740267	23.60227027526895	23.119663388921428	25.811280478406957
27	27.985997657285534	22.92951049597731	22.38563854381801	26.698853302919147
28	26.53470916432909	22.618754045806234	23.780285749514505	27.066251040350174
29	26.17193520544989	22.876529700070897	24.24266360469776	26.70887148978145
30	25.905682623840203	23.68954409543479	23.79396442773034	26.610808852994666
31	26.75973305385161	23.47858419900743	23.094617921765668	26.6670648253753
32	26.136678893992173	23.06745322277365	23.28727536142536	27.508592521808822
33	26.469976264603435	23.146828087913445	23.24758792885546	27.135607718627664
34	27.448098085755678	22.947234980426003	23.19884559662156	26.405821337196755
35	27.33134767732191	22.871327949200086	23.91880644862982	25.87851792484819
36	26.473444098517305	23.741368946703247	22.68734009432508	27.09784686045436
37	26.469205634844794	22.93143707037391	23.6689297493912	26.930427545390096
38	26.527966153941	22.809870225948643	24.20914121019697	26.45302240991338
39	25.752327301871087	22.768256218982152	24.393899694830615	27.085516784316145
40	26.04863444406769	22.115725470854784	25.4483138620881	26.387326222989426
41	24.86359853272094	22.31358466138528	25.836903917881692	26.98591288801208
42	25.33483863012854	22.66980826731605	25.39321383434543	26.602139268209978
43	24.90636848432539	21.032027372769026	26.29716254122869	27.764441601676893
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	8.5
2	11.0
3	21.0
4	31.0
5	31.0
6	42.0
7	53.0
8	51.0
9	49.0
10	60.5
11	72.0
12	72.0
13	162.0
14	252.0
15	376.0
16	500.0
17	513.5
18	527.0
19	527.0
20	739.0
21	951.0
22	1110.5
23	1270.0
24	1612.0
25	1954.0
26	1954.0
27	2648.5
28	3343.0
29	4274.0
30	5205.0
31	6633.5
32	8062.0
33	8062.0
34	9907.0
35	11752.0
36	14073.0
37	16394.0
38	18437.0
39	20480.0
40	20480.0
41	22666.5
42	24853.0
43	26761.5
44	28670.0
45	30627.5
46	32585.0
47	32585.0
48	34108.5
49	35632.0
50	37249.0
51	38866.0
52	41799.5
53	44733.0
54	44733.0
55	44677.5
56	44622.0
57	42567.0
58	40512.0
59	37596.0
60	34680.0
61	34680.0
62	33520.0
63	32360.0
64	28981.5
65	25603.0
66	22016.0
67	18429.0
68	18429.0
69	15501.5
70	12574.0
71	11438.0
72	10302.0
73	9819.5
74	9337.0
75	9337.0
76	8548.0
77	7759.0
78	5972.0
79	4185.0
80	2772.0
81	1359.0
82	1359.0
83	926.0
84	493.0
85	386.0
86	279.0
87	226.0
88	173.0
89	173.0
90	141.0
91	109.0
92	61.5
93	14.0
94	12.5
95	11.0
96	11.0
97	7.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	519056.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.02644789963435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.97818468559477	52.810179193342634
2	6.678257712474972	8.017441772764204
3	1.7977906720482189	3.237449643304531
4	0.8064295006701616	1.9362839362682243
5	0.5043124089850346	1.5136041271539638
6	0.3431441807790866	1.2358635773759132
7	0.2519096957854497	1.0584870960634625
8	0.18926864292333156	0.9088899466777489
9	0.15170721972428633	0.8195800968700429
>10	1.0877595742637618	12.806994569287838
>50	0.1280897373636925	5.420487864078038
>100	0.08025672720784408	8.933235283941848
>500	0.00256821527065101	0.9621563435161493
>1k	3.2102690883137623E-4	0.33934654935548547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1761	0.3392697512407139	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	933	0.17974939120249067	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	721	0.1389060139946364	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	595	0.1146311765975155	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	565	0.1088514534077248	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	548	0.10557627693351006	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	548	0.10557627693351006	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	546	0.10519096205419069	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	537	0.10345704509725348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.926574396596899E-4	0.0	0.0	0.0	0.0
11	1.926574396596899E-4	0.0	0.0	0.0	0.0
12	1.926574396596899E-4	0.0	0.0	0.0	0.0
13	1.926574396596899E-4	0.0	0.0	0.0	0.0
14	1.926574396596899E-4	0.0	0.0	0.0	0.0
15	1.926574396596899E-4	0.0	0.0	0.0	0.0
16	1.926574396596899E-4	0.0	0.0	1.926574396596899E-4	0.0
17	1.926574396596899E-4	0.0	0.0	1.926574396596899E-4	0.0
18	1.926574396596899E-4	0.0	0.0	1.926574396596899E-4	0.0
19	1.926574396596899E-4	0.0	0.0	5.779723189790697E-4	0.0
20	1.926574396596899E-4	0.0	0.0	5.779723189790697E-4	0.0
21	1.926574396596899E-4	0.0	0.0	7.706297586387596E-4	0.0
22	1.926574396596899E-4	0.0	0.0	0.0013486020776178292	0.0
23	1.926574396596899E-4	0.0	0.0	0.0023118892759162787	0.0
24	1.926574396596899E-4	0.0	0.0	0.003853148793193798	0.0
25	1.926574396596899E-4	0.0	0.0	0.005009093431151937	0.0
26	1.926574396596899E-4	0.0	0.0	0.0067430103880891466	0.0
27	1.926574396596899E-4	0.0	0.0	0.013678678215837983	1.926574396596899E-4
28	1.926574396596899E-4	0.0	0.0	0.03718288585432015	1.926574396596899E-4
29	1.926574396596899E-4	0.0	0.0	0.0743657717086403	1.926574396596899E-4
30	1.926574396596899E-4	0.0	0.0	0.1259979655374372	1.926574396596899E-4
31	1.926574396596899E-4	0.0	0.0	0.26239943281649764	1.926574396596899E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGAT	20	0.0018410065	37.0	3
CAAGGGT	35	8.8628184E-4	26.42857	4
CGCAAGA	80	9.749783E-10	25.4375	2
GGTATCA	240	0.0	23.895834	1
ACGTTCG	70	1.9187064E-7	23.785713	7
ATTAGAC	55	1.9000532E-5	23.545454	3
AAGACGG	95	2.8012437E-10	23.368422	5
TATTTCG	50	2.6993363E-4	22.2	27
TTAGACT	50	2.6993363E-4	22.2	4
CAGACGT	70	5.093034E-6	21.142857	4
TTCTATA	45	0.003823024	20.555555	2
TTATATA	45	0.003823024	20.555555	4
GCTTAGA	45	0.003823024	20.555555	1
GCATCAG	455	0.0	20.32967	15
TGCATCA	475	0.0	20.252634	14
TATTAGA	55	5.137872E-4	20.181818	2
CAAGACG	110	1.7480488E-9	20.181818	4
CAGGACA	525	0.0	20.085714	4
CCAGGAC	530	0.0	19.896227	3
CGTGTTG	75	9.2497285E-6	19.733332	34
>>END_MODULE