FastQCFastQC Report
Fri 10 Feb 2017
ERR1630136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11382
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC190.16693024073097873No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC140.12300123001230012No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA140.12300123001230012No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA130.11421542786856441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200.0017662337.01
AACGCAG250.005272958429.67
GTATCAA250.005272958429.62
TCAACGC250.005272958429.65
CAGAGTA250.005272958429.611
AGAGTAC250.005272958429.612
GCAGAGT250.005272958429.610
CGCAGAG250.005272958429.69
ATCAACG250.005272958429.64
CAACGCA250.005272958429.66
CTGTCTC500.00662487718.537
Produced by FastQC (version 0.11.5)