FastQCFastQC Report
Fri 10 Feb 2017
ERR1630135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149800
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20251.3518024032042724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19801.321762349799733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11970.7990654205607477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.49198931909212285No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2730.1822429906542056No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2500.16688918558077437No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.16688918558077437No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.16088117489986647No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2070.1381842456608812No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1850.12349799732977304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.12149532710280374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTT251.2285072E-437.024
GTACTAG451.0693293E-732.888891
ATCGTCA401.5878013E-632.37510
TGCCCAC250.00548128629.611
GAATGTT250.00548128629.627
TTTAAAT250.00548128629.63
AATGCCA250.00548128629.69
AGGCATC502.6941598E-729.66
CAATGCG453.977746E-628.77777719
TGATCAC358.8332844E-426.42857221
TTGATCA358.8332844E-426.42857220
ACGTTTA358.8332844E-426.42857226
CACGTTT358.8332844E-426.42857225
CGTTTAT358.8332844E-426.42857227
CGACGAG509.022369E-625.90000224
TACTAGG659.278301E-825.6153852
TCTTATA1850.025.037
GTCATCC551.8883784E-523.54545413
ACGAGTC551.8883784E-523.54545426
AGGAGCG400.001923399423.12500233
Produced by FastQC (version 0.11.5)