##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630135.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	149800
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.705634178905207	31.0	31.0	34.0	30.0	34.0
2	31.862242990654206	33.0	31.0	34.0	30.0	34.0
3	31.919819759679573	33.0	31.0	34.0	30.0	34.0
4	35.63305073431242	37.0	35.0	37.0	33.0	37.0
5	35.60380507343124	37.0	35.0	37.0	33.0	37.0
6	35.69753671562083	37.0	35.0	37.0	33.0	37.0
7	35.695293724966625	37.0	35.0	37.0	33.0	37.0
8	35.701855807743655	37.0	35.0	37.0	33.0	37.0
9	37.345574098798394	39.0	37.0	39.0	34.0	39.0
10	37.25111481975968	39.0	37.0	39.0	34.0	39.0
11	37.34127503337784	39.0	37.0	39.0	34.0	39.0
12	37.23642857142857	39.0	37.0	39.0	34.0	39.0
13	37.27510680907877	39.0	37.0	39.0	34.0	39.0
14	38.34654205607477	40.0	38.0	41.0	34.0	41.0
15	38.35072096128171	40.0	38.0	41.0	34.0	41.0
16	38.226728971962615	40.0	38.0	41.0	33.0	41.0
17	38.293377837116154	40.0	38.0	41.0	34.0	41.0
18	38.35	40.0	38.0	41.0	34.0	41.0
19	38.37618825100134	40.0	38.0	41.0	34.0	41.0
20	38.358437917222965	40.0	38.0	41.0	34.0	41.0
21	38.34753004005341	40.0	38.0	41.0	34.0	41.0
22	38.30186248331108	40.0	38.0	41.0	34.0	41.0
23	38.24892523364486	40.0	38.0	41.0	34.0	41.0
24	38.215507343124166	40.0	38.0	41.0	33.0	41.0
25	38.16488651535381	40.0	37.0	41.0	33.0	41.0
26	37.93766355140187	40.0	37.0	41.0	33.0	41.0
27	37.80128838451268	40.0	37.0	41.0	33.0	41.0
28	37.71407209612817	40.0	37.0	41.0	33.0	41.0
29	37.59353137516689	39.0	37.0	41.0	33.0	41.0
30	37.448731642189586	39.0	36.0	41.0	32.0	41.0
31	37.24269692923899	39.0	36.0	41.0	32.0	41.0
32	37.082383177570094	39.0	35.0	41.0	31.0	41.0
33	36.97345794392523	39.0	35.0	41.0	31.0	41.0
34	36.83576769025367	39.0	35.0	41.0	31.0	41.0
35	36.679038718291054	39.0	35.0	40.0	31.0	41.0
36	36.526428571428575	39.0	35.0	40.0	30.0	41.0
37	36.39853137516689	39.0	35.0	40.0	30.0	41.0
38	36.23393858477971	39.0	35.0	40.0	30.0	41.0
39	36.03837116154873	38.0	35.0	40.0	29.0	41.0
40	35.840514018691586	38.0	35.0	40.0	28.0	41.0
41	35.57700267022697	38.0	35.0	40.0	27.0	41.0
42	35.435821094793056	38.0	35.0	40.0	26.0	41.0
43	34.26164218958611	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	0.0
13	0.0
14	0.0
15	2.0
16	1.0
17	4.0
18	10.0
19	16.0
20	22.0
21	56.0
22	143.0
23	185.0
24	307.0
25	535.0
26	824.0
27	1184.0
28	1619.0
29	2130.0
30	2852.0
31	3766.0
32	4669.0
33	5976.0
34	7776.0
35	10173.0
36	14486.0
37	22623.0
38	35008.0
39	35429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.735647530040055	19.85113484646195	13.680240320427236	22.73297730307076
2	19.491989319092124	22.609479305740987	33.81642189586115	24.082109479305743
3	20.641522029372496	25.275700934579437	30.866488651535377	23.216288384512684
4	14.136181575433913	17.85781041388518	34.735647530040055	33.27036048064085
5	12.96328437917223	39.26902536715621	33.70493991989319	14.06275033377837
6	30.23631508678238	34.988651535380505	17.433911882510014	17.3411214953271
7	26.667556742323097	32.73898531375167	21.78237650200267	18.811081441922564
8	28.86181575433912	32.20961281708945	19.829105473965285	19.09946595460614
9	27.733644859813083	12.937917222963952	20.746995994659546	38.58144192256342
10	20.39385847797063	27.315754339118826	29.5347129506008	22.755674232309747
11	33.02336448598131	24.523364485981308	19.114819759679573	23.33845126835781
12	20.67423230974633	29.643524699599467	27.41989319092123	22.262349799732977
13	31.95060080106809	19.82109479305741	25.545393858477972	22.682910547396528
14	23.188918558077436	22.37249666221629	26.82109479305741	27.617489986648863
15	28.905206942590123	25.75033377837116	22.987983978638184	22.356475300400533
16	23.072096128170895	25.224299065420563	27.673564753004005	24.030040053404537
17	20.914552736982646	28.65287049399199	26.70293724966622	23.729639519359143
18	21.008678237650198	24.664886515353803	28.927236315086784	25.39919893190921
19	22.01468624833111	26.956608811749	30.265687583444596	20.7630173564753
20	23.09279038718291	24.08010680907877	31.38584779706275	21.441255006675565
21	24.483311081441922	24.697596795727637	28.98064085447263	21.83845126835781
22	24.58611481975968	24.65754339118825	28.06742323097463	22.688918558077436
23	23.378504672897197	25.76769025367156	28.383845126835784	22.46995994659546
24	21.60547396528705	27.12950600801068	28.519359145527368	22.7456608811749
25	22.812416555407207	26.086114819759683	29.14419225634179	21.95727636849132
26	23.295060080106808	25.85580774365821	28.44526034712951	22.403871829105473
27	22.305073431241656	26.44659546061415	28.403871829105476	22.844459279038716
28	21.48064085447263	26.963951935914555	30.035380507343124	21.520026702269693
29	21.97463284379172	26.983311081441926	29.259012016021362	21.783044058744995
30	21.591455273698266	28.463284379172233	29.68758344459279	20.257676902536716
31	22.261682242990656	27.23965287049399	28.82710280373832	21.671562082777037
32	20.15487316421896	28.110814419225633	29.0694259012016	22.664886515353803
33	20.914552736982646	27.64886515353805	29.77503337783712	21.66154873164219
34	21.2977303070761	27.299065420560748	28.640854472630174	22.762349799732977
35	21.567423230974633	28.481975967957275	28.64419225634179	21.306408544726303
36	20.774365821094793	28.437917222963954	27.833110814419225	22.95460614152203
37	21.16555407209613	28.076769025367156	28.680240320427234	22.07743658210948
38	21.014686248331106	27.66688918558077	28.832443257676903	22.485981308411215
39	20.704939919893192	27.15086782376502	29.955941255006675	22.188251001335114
40	20.57943925233645	27.417222963951936	30.09479305740988	21.908544726301734
41	19.68157543391188	27.359145527369826	30.29706275033378	22.662216288384514
42	19.08411214953271	27.99265687583445	30.28371161548732	22.639519359145527
43	19.615487316421895	27.85580774365821	30.176234979973298	22.352469959946593
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	20.5
2	29.0
3	111.0
4	193.0
5	193.0
6	293.0
7	393.0
8	394.0
9	395.0
10	464.5
11	534.0
12	534.0
13	1152.5
14	1771.0
15	2853.5
16	3936.0
17	3199.5
18	2463.0
19	2463.0
20	2972.5
21	3482.0
22	2364.0
23	1246.0
24	1345.0
25	1444.0
26	1444.0
27	1635.0
28	1826.0
29	2009.0
30	2192.0
31	2637.5
32	3083.0
33	3083.0
34	3802.0
35	4521.0
36	5070.0
37	5619.0
38	6262.5
39	6906.0
40	6906.0
41	8028.5
42	9151.0
43	10352.5
44	11554.0
45	12722.0
46	13890.0
47	13890.0
48	15557.0
49	17224.0
50	15715.0
51	14206.0
52	12557.5
53	10909.0
54	10909.0
55	10033.5
56	9158.0
57	8154.5
58	7151.0
59	6195.0
60	5239.0
61	5239.0
62	4384.0
63	3529.0
64	2860.5
65	2192.0
66	1809.5
67	1427.0
68	1427.0
69	1235.0
70	1043.0
71	883.0
72	723.0
73	698.5
74	674.0
75	674.0
76	609.5
77	545.0
78	497.0
79	449.0
80	345.0
81	241.0
82	241.0
83	210.0
84	179.0
85	150.0
86	121.0
87	110.5
88	100.0
89	100.0
90	70.0
91	40.0
92	23.5
93	7.0
94	5.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	149800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.381842456608815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50378004542834	27.765687583444592
2	11.853747838763264	9.33644859813084
3	5.04627589246364	5.961949265687584
4	2.8494423161677456	4.488651535380507
5	1.8222192087330915	3.588117489986649
6	1.3526799335525646	3.196261682242991
7	1.0492592467030544	2.892523364485981
8	0.8339831169271452	2.6275033377837116
9	0.6153168118791742	2.1809078771695596
>10	3.854629284334	26.84045393858478
>50	0.15425297487880124	4.0046728971962615
>100	0.05763297962504661	3.1522029372496667
>500	0.0016950876360307828	0.49198931909212285
>1k	0.005085262908092348	3.472630173564753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2025	1.3518024032042724	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1980	1.321762349799733	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.7990654205607477	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	737	0.49198931909212285	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	273	0.1822429906542056	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	250	0.16688918558077437	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	250	0.16688918558077437	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.16088117489986647	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	207	0.1381842456608812	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	185	0.12349799732977304	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	182	0.12149532710280374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	6.675567423230974E-4	0.0
13	0.0	0.0	0.0	6.675567423230974E-4	0.0
14	0.0	0.0	0.0	0.0013351134846461949	0.0
15	0.0	0.0	0.0	0.0026702269692923898	0.0
16	0.0	0.0	0.0	0.0053404539385847796	0.0
17	0.0	0.0	0.0	0.012016021361815754	0.0
18	0.0	0.0	0.0	0.014686248331108143	0.0
19	0.0	0.0	0.0	0.016688918558077435	0.0
20	0.0	0.0	0.0	0.016688918558077435	0.0
21	0.0	0.0	0.0	0.022029372496662217	0.0
22	0.0	0.0	0.0	0.03337783711615487	0.0
23	0.0	0.0	0.0	0.0514018691588785	0.0
24	0.0	0.0	0.0	0.07610146862483311	0.0
25	0.0	0.0	0.0	0.08945260347129506	0.0
26	0.0	0.0	0.0	0.1041388518024032	0.0
27	0.0	0.0	0.0	0.1261682242990654	0.0
28	0.0	0.0	0.0	0.1542056074766355	0.0
29	0.0	0.0	0.0	0.19559412550066757	0.0
30	0.0	0.0	0.0	0.27903871829105475	0.0
31	0.0	0.0	0.0	0.5587449933244326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGTT	25	1.2285072E-4	37.0	24
GTACTAG	45	1.0693293E-7	32.88889	1
ATCGTCA	40	1.5878013E-6	32.375	10
TGCCCAC	25	0.005481286	29.6	11
GAATGTT	25	0.005481286	29.6	27
TTTAAAT	25	0.005481286	29.6	3
AATGCCA	25	0.005481286	29.6	9
AGGCATC	50	2.6941598E-7	29.6	6
CAATGCG	45	3.977746E-6	28.777777	19
TGATCAC	35	8.8332844E-4	26.428572	21
TTGATCA	35	8.8332844E-4	26.428572	20
ACGTTTA	35	8.8332844E-4	26.428572	26
CACGTTT	35	8.8332844E-4	26.428572	25
CGTTTAT	35	8.8332844E-4	26.428572	27
CGACGAG	50	9.022369E-6	25.900002	24
TACTAGG	65	9.278301E-8	25.615385	2
TCTTATA	185	0.0	25.0	37
GTCATCC	55	1.8883784E-5	23.545454	13
ACGAGTC	55	1.8883784E-5	23.545454	26
AGGAGCG	40	0.0019233994	23.125002	33
>>END_MODULE