FastQCFastQC Report
Fri 10 Feb 2017
ERR1630134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664893
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC31150.46849643476469144No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT21820.328173104544641No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20330.3057634837485129No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA18740.2818498615566715No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG17070.2567330382482595No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG14930.22454740837999498No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG14920.22439700824042363No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG14530.2185314027971418No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG12390.18634577292887727No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA12080.18168336860216605No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG11840.17807376525245414No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG11230.16889935673860304No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA10970.16498895310974848No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC10650.16017614864346594No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG10230.1538593427814701No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC10190.15325774222318478No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC9810.14754253691947425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.145136134686333No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA9550.1436321332906197No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA9470.14242893217404906No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG9320.14017293008047912No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT8950.13460812491633992No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG8750.13160012212491334No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC8730.13129932184577067No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT8510.12799051877520143No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA8470.1273889182169161No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA8270.12438091542548951No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA8220.12362891472763288No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC8130.1222753134714909No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC8120.12212491333191958No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG8050.12107211235492027No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC8030.1207713120757776No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC7700.11580810746992373No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT7540.11340170523678247No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC7400.11129610328278386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7370.11084490286406987No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG7340.11039370244535587No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG7280.10949130160792789No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG7240.10888970104964257No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT7000.10528009769993067No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC6890.10362569616464604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6720.10106889379193344No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA6710.1009184936523621No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCT358.865447E-426.428576
CTAGTAC358.865447E-426.428573
ATACTTA400.001930335823.1256
GCGGGTA802.72139E-823.12523
TAGTACC400.001930335823.1254
GCTTTAT4000.021.7375011
GGTATCA4750.021.4210531
AACCCGT1151.3460522E-1020.91304429
CCCGTTG1151.3460522E-1020.91304431
CGCCCTA450.003824137820.55555516
TAACCCG1001.2872988E-820.3528
GTATTGG555.1399594E-420.1818181
TTCGTTC656.8965375E-519.92307729
CGGGTAA951.673452E-719.47368424
CAAGACG1052.255183E-819.380954
TTTATTG4950.019.0606063
GGTAACC1103.8442522E-818.526
GTCCTAA609.2312327E-418.51
AGAGTGT1002.8704017E-718.56
GTAGAAC500.007031837518.53
Produced by FastQC (version 0.11.5)