FastQCFastQC Report
Fri 10 Feb 2017
ERR1630133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590086
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT228511.437092081811927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT172441.084469645038067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT159251.001518156879565No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74810.47047769743271745No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.14187911848793083No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.1322569974202653No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19430.12219464859133405No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA17890.1125096378434877No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA17030.10710112534793716No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.10546599366323582No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.1047742071812468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTG250.005496969429.627
CCCAACG400.001931510923.12500221
GCGTCGT400.001931510923.12500216
TCCGTAT1301.8189894E-1221.34615313
AGTTCGT450.003826442920.55555733
TTACTCG1650.020.18181819
TACTCGC1202.382876E-1020.04166820
CGTCGTC656.904257E-519.92307717
AATCGGG759.269439E-619.73333223
CGCAAGA2200.019.3409082
ACTCGCA1254.129106E-1019.2421
GATCGAT500.007036039618.516
CAGTTCG609.2389365E-418.532
CGCGCGA609.2389365E-418.532
CCGTATT1306.9667294E-1018.514
ACCCGTA1002.8766408E-718.521
GGTATCA71550.018.0216641
TACCGTC1850.018.07
CATCCGT1351.1532393E-917.81481411
AAGACGG2300.017.6956525
Produced by FastQC (version 0.11.5)