##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630133.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1590086
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80656958177105	31.0	31.0	34.0	30.0	34.0
2	31.95665643241938	33.0	31.0	34.0	30.0	34.0
3	32.08524067251708	33.0	31.0	34.0	30.0	34.0
4	35.7546239637353	37.0	35.0	37.0	33.0	37.0
5	35.69600197725155	37.0	35.0	37.0	33.0	37.0
6	35.78616313834598	37.0	35.0	37.0	35.0	37.0
7	35.766337795565775	37.0	35.0	37.0	35.0	37.0
8	35.77439585028734	37.0	35.0	37.0	35.0	37.0
9	37.448266949083255	39.0	37.0	39.0	35.0	39.0
10	37.35797057517644	39.0	37.0	39.0	34.0	39.0
11	37.450403311519	39.0	37.0	39.0	35.0	39.0
12	37.330646266931474	39.0	37.0	39.0	34.0	39.0
13	37.38445467729419	39.0	37.0	39.0	34.0	39.0
14	38.50662920118786	40.0	38.0	41.0	34.0	41.0
15	38.49137782484721	40.0	38.0	41.0	34.0	41.0
16	38.4741938486346	40.0	38.0	41.0	34.0	41.0
17	38.50774549301107	40.0	38.0	41.0	34.0	41.0
18	38.53303091782457	40.0	38.0	41.0	34.0	41.0
19	38.57266776765533	40.0	38.0	41.0	34.0	41.0
20	38.53772060127565	40.0	38.0	41.0	34.0	41.0
21	38.50172003275294	40.0	38.0	41.0	34.0	41.0
22	38.451515201064595	40.0	38.0	41.0	34.0	41.0
23	38.37222766567343	40.0	38.0	41.0	34.0	41.0
24	38.33599314754045	40.0	38.0	41.0	34.0	41.0
25	38.309615328982204	40.0	38.0	41.0	34.0	41.0
26	38.1347367375098	40.0	37.0	41.0	33.0	41.0
27	38.01277352294153	40.0	37.0	41.0	33.0	41.0
28	37.89609870157966	40.0	37.0	41.0	33.0	41.0
29	37.78739514718072	40.0	37.0	41.0	33.0	41.0
30	37.654716788903244	40.0	37.0	41.0	33.0	41.0
31	37.4685249728631	39.0	36.0	41.0	33.0	41.0
32	37.30663624483204	39.0	36.0	41.0	32.0	41.0
33	37.17849726367002	39.0	35.0	41.0	32.0	41.0
34	37.06071432614337	39.0	35.0	41.0	31.0	41.0
35	36.913319153806775	39.0	35.0	41.0	31.0	41.0
36	36.7660321517201	39.0	35.0	41.0	31.0	41.0
37	36.649169290214495	39.0	35.0	41.0	31.0	41.0
38	36.485650461673146	39.0	35.0	41.0	30.0	41.0
39	36.3258905493162	39.0	35.0	40.0	30.0	41.0
40	36.14302056618321	39.0	35.0	40.0	30.0	41.0
41	35.92809256857805	39.0	35.0	40.0	29.0	41.0
42	35.75780492375884	39.0	35.0	40.0	27.0	41.0
43	34.6630496715272	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	20.0
11	22.0
12	9.0
13	8.0
14	14.0
15	8.0
16	15.0
17	24.0
18	60.0
19	131.0
20	313.0
21	536.0
22	947.0
23	1762.0
24	2822.0
25	4563.0
26	7167.0
27	10733.0
28	15215.0
29	20975.0
30	27842.0
31	36322.0
32	46517.0
33	59811.0
34	78657.0
35	105014.0
36	148270.0
37	232133.0
38	355049.0
39	435116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.367726022366085	19.828298595170324	13.30833678178413	22.49563860067946
2	17.730235974657973	23.13944025669052	35.05187769718116	24.078446071470346
3	19.49768754645975	25.86772036229487	30.880279431426978	23.7543126598184
4	12.894459796514152	16.425904007707757	36.14898816793557	34.53064802784252
5	12.309648660512702	40.371086846874945	33.58478723792298	13.73447725468937
6	30.547278574869534	37.08258547021985	17.18642891013442	15.183707044776195
7	27.135576314740206	33.28159608977124	21.71605812515801	17.866769470330535
8	29.006481410439434	32.806275886964606	20.16280880405211	18.02443389854385
9	27.653535720709442	13.391980056424622	20.9930783617993	37.96140586106663
10	17.92160927144821	28.367333590761756	31.733566612120352	21.97749052566968
11	33.881123410935004	23.68771248850691	21.3521155459516	21.07904855460648
12	20.8032144173334	29.57701658903984	29.04295742494431	20.576811568682448
13	29.955926912129282	20.553479497335363	26.70364999125833	22.78694359927702
14	22.269361531388867	22.271374001154655	27.74963115202574	27.709633315430736
15	24.688853307305394	28.859885565937944	24.54678551977692	21.904475606979748
16	22.57544560482892	27.34581651558469	26.573531242964215	23.50520663662217
17	20.816358360491193	28.60644015480924	27.68523211952058	22.89196936517899
18	20.854532396360952	25.740054311527803	30.280437661862315	23.12497563024893
19	21.43682794515517	26.66799154259581	31.10806585304191	20.787114659207113
20	22.54236563305381	25.70288651054094	30.74940600697069	21.005341849434558
21	23.621112317195422	26.1551890904014	29.456834410214288	20.766864182188886
22	23.12208270496061	26.01047993630533	29.775936647451772	21.091500711282283
23	21.90076511584908	26.39140272916056	30.081832051851283	21.626000103139077
24	20.883147200843226	27.536938253654203	30.048186072954547	21.531728472548025
25	21.470473924051905	26.82225992807936	30.25540756915035	21.451858578718383
26	21.1573462064316	27.762837984863715	29.994352506719764	21.085463301984923
27	21.384755289965447	27.16243020817742	29.815494256285508	21.637320245571622
28	20.837929520793214	27.426189526855783	30.898077210918153	20.837803741432854
29	20.7678075273916	27.440905712018093	30.717458049438832	21.07382871115147
30	20.394179937437347	28.48871067351074	30.37005545611998	20.74705393293193
31	21.19319332413467	28.278784921067164	29.7489569746542	20.779064780143965
32	20.420153375352022	27.864593487396277	29.97359891226009	21.741654224991606
33	20.037909899213	27.812080604445296	30.505016709788023	21.644992786553683
34	21.209921979062766	27.099666307356962	30.20063065771285	21.489781055867418
35	20.963708881154854	27.44625133483346	30.59507473180696	20.994965052204723
36	20.46662884900565	28.208348479264643	29.546263535431414	21.77875913629829
37	21.206525936333005	27.299844159372512	29.805997914578203	21.68763198971628
38	20.457761404100154	27.453735206774983	30.332761875772757	21.75574151335211
39	20.793214958184652	27.130608030005927	30.28402237363262	21.792154638176804
40	20.298147395801234	26.763709635831017	31.19711764017795	21.741025328189796
41	19.774968146376988	27.110860670429147	31.19227513480403	21.921896048389836
42	19.96841680261319	26.95149822085095	31.23573190380898	21.844353072726886
43	19.69012996781306	26.081419495549298	31.22485198913769	23.003598547499944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1385.0
1	1177.0
2	969.0
3	2055.5
4	3142.0
5	3142.0
6	4253.0
7	5364.0
8	4978.0
9	4592.0
10	6560.0
11	8528.0
12	8528.0
13	14640.0
14	20752.0
15	32353.0
16	43954.0
17	38971.5
18	33989.0
19	33989.0
20	36253.5
21	38518.0
22	30780.0
23	23042.0
24	24491.5
25	25941.0
26	25941.0
27	28852.0
28	31763.0
29	37570.0
30	43377.0
31	47279.0
32	51181.0
33	51181.0
34	56799.5
35	62418.0
36	69073.5
37	75729.0
38	80088.5
39	84448.0
40	84448.0
41	92516.0
42	100584.0
43	102460.5
44	104337.0
45	107263.0
46	110189.0
47	110189.0
48	114200.0
49	118211.0
50	111416.0
51	104621.0
52	102892.5
53	101164.0
54	101164.0
55	95490.5
56	89817.0
57	83594.0
58	77371.0
59	71372.5
60	65374.0
61	65374.0
62	58965.5
63	52557.0
64	45605.5
65	38654.0
66	32832.0
67	27010.0
68	27010.0
69	21807.5
70	16605.0
71	13963.5
72	11322.0
73	8495.5
74	5669.0
75	5669.0
76	4148.0
77	2627.0
78	2129.0
79	1631.0
80	1403.0
81	1175.0
82	1175.0
83	904.0
84	633.0
85	599.5
86	566.0
87	508.0
88	450.0
89	450.0
90	401.0
91	352.0
92	196.5
93	41.0
94	33.0
95	25.0
96	25.0
97	16.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1590086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.83536836989223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09925450415899	38.4514882759582
2	10.209810334061675	9.56360455965022
3	2.8949069341299314	4.06752097969592
4	1.1800087195649833	2.210645722420434
5	0.6577063141335419	1.5401958750824245
6	0.41793675129725677	1.1744533021393835
7	0.29606314084667423	0.9706358383610891
8	0.23698531715372423	0.8879435701720331
9	0.18412953576602656	0.7761397171841189
>10	1.4740881378742958	14.089093128868985
>50	0.19564669290255532	6.433912981166036
>100	0.14417506986977033	12.205470827948995
>500	0.006461598776054956	2.0368981902317405
>1k	0.0022884828998527965	1.5883732970101907
>5k	1.3461664116781158E-4	0.47166358254011975
>10k+	4.0384992350343476E-4	3.5319601515703125
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22851	1.437092081811927	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17244	1.084469645038067	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15925	1.001518156879565	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7481	0.47047769743271745	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2256	0.14187911848793083	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2103	0.1322569974202653	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1943	0.12219464859133405	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1789	0.1125096378434877	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1703	0.10710112534793716	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1677	0.10546599366323582	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1666	0.1047742071812468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.288968018082041E-5	0.0	0.0	0.0
3	0.0	6.288968018082041E-5	0.0	0.0	0.0
4	0.0	6.288968018082041E-5	0.0	0.0	0.0
5	0.0	2.5155872072328165E-4	0.0	0.0	0.0
6	0.0	2.5155872072328165E-4	0.0	0.0	0.0
7	0.0	2.5155872072328165E-4	0.0	0.0	0.0
8	0.0	2.5155872072328165E-4	0.0	0.0	0.0
9	0.0	2.5155872072328165E-4	0.0	6.288968018082041E-5	0.0
10	0.0	2.5155872072328165E-4	0.0	3.1444840090410207E-4	0.0
11	0.0	2.5155872072328165E-4	0.0	8.175658423506653E-4	0.0
12	0.0	2.5155872072328165E-4	0.0	0.0013206832837972286	0.0
13	0.0	3.1444840090410207E-4	0.0	0.0018866904054246122	0.0
14	0.0	3.1444840090410207E-4	0.0	0.002138249126147894	0.0
15	0.0	7.546761621698449E-4	0.0	0.0033331530495834816	0.0
16	0.0	7.546761621698449E-4	0.0	0.005597181536093016	0.0
17	0.0	7.546761621698449E-4	0.0	0.008741665545134036	0.0
18	0.0	7.546761621698449E-4	0.0	0.010062348828931266	0.0
19	0.0	7.546761621698449E-4	0.0	0.012137708274898339	0.0
20	0.0	7.546761621698449E-4	0.0	0.01389861931996131	0.0
21	6.288968018082041E-5	7.546761621698449E-4	0.0	0.019621580216415968	0.0
22	6.288968018082041E-5	7.546761621698449E-4	0.0	0.030375715527336256	0.0
23	6.288968018082041E-5	7.546761621698449E-4	0.0	0.04546923877073315	0.0
24	6.288968018082041E-5	7.546761621698449E-4	0.0	0.06408458410425599	0.0
25	6.288968018082041E-5	7.546761621698449E-4	0.0	0.07005910372143394	0.0
26	6.288968018082041E-5	7.546761621698449E-4	0.0	0.07898943830711043	0.0
27	6.288968018082041E-5	7.546761621698449E-4	0.0	0.10206995093347153	0.0
28	6.288968018082041E-5	7.546761621698449E-4	0.0	0.15546328940698806	0.0
29	6.288968018082041E-5	7.546761621698449E-4	0.0	0.2320629198672273	0.0
30	6.288968018082041E-5	7.546761621698449E-4	0.0	0.35740205246760237	0.0
31	6.288968018082041E-5	7.546761621698449E-4	0.0	0.6495246169075132	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGTG	25	0.0054969694	29.6	27
CCCAACG	40	0.0019315109	23.125002	21
GCGTCGT	40	0.0019315109	23.125002	16
TCCGTAT	130	1.8189894E-12	21.346153	13
AGTTCGT	45	0.0038264429	20.555557	33
TTACTCG	165	0.0	20.181818	19
TACTCGC	120	2.382876E-10	20.041668	20
CGTCGTC	65	6.904257E-5	19.923077	17
AATCGGG	75	9.269439E-6	19.733332	23
CGCAAGA	220	0.0	19.340908	2
ACTCGCA	125	4.129106E-10	19.24	21
GATCGAT	50	0.0070360396	18.5	16
CAGTTCG	60	9.2389365E-4	18.5	32
CGCGCGA	60	9.2389365E-4	18.5	32
CCGTATT	130	6.9667294E-10	18.5	14
ACCCGTA	100	2.8766408E-7	18.5	21
GGTATCA	7155	0.0	18.021664	1
TACCGTC	185	0.0	18.0	7
CATCCGT	135	1.1532393E-9	17.814814	11
AAGACGG	230	0.0	17.695652	5
>>END_MODULE