FastQCFastQC Report
Fri 10 Feb 2017
ERR1630132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983811
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121530.6126087616209407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115760.5835233295913774No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC110690.5579664595064752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82140.4140515401920848No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT63670.32094791288081376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59310.29897001276835344No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG40690.20511026504036928No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT40630.20480781687368405No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG38070.19190336176178072No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT35930.18111604381667407No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT34130.17204259881611705No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG32900.16584241139906977No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT32310.16286833775999832No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG32140.16201140128772348No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG29110.14673776887011916No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG28110.14169696609203195No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT28090.1415961500364702No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC27140.13680738739728734No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC26450.13332923348040718No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG25910.13060719998024006No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG24700.12450782861875452No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT24210.12203783525749176No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT23320.11755152078499413No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG22220.11200663772909819No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG21830.11004072464564417No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA21360.10767154733994316No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.10580645031205091No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT20440.10303400878410292No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA20030.10096727964508714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATCA26400.018.9905314
CCAGGAC28050.018.7967913
CAGGACA28250.018.7292044
GACAGGC28150.018.401427
GCATCAG27600.018.29891415
TCCAGGA30100.017.8853842
CGGACCA2700.017.8148169
GCTGCAT28150.017.5470712
ACAGGCT29900.017.3862888
GGACAGG31400.017.3216556
CTGCATC28650.017.3054113
ACGGACC2900.017.2241388
CGCGCTA1950.017.07692324
AAGACGG2850.016.8771935
AGGCCAT29550.016.84094825
ATCAAGC29550.016.6531330
GGCCATC30000.016.52666726
GACGGAC2800.016.5178577
ATACCGT1801.0913936E-1116.4444456
CGACGGT1606.311893E-1016.18757
Produced by FastQC (version 0.11.5)