##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630132.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1983811
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8638993331522	33.0	31.0	34.0	30.0	34.0
2	32.0463164081659	33.0	31.0	34.0	30.0	34.0
3	32.16164291860464	34.0	31.0	34.0	30.0	34.0
4	35.819093653578896	37.0	35.0	37.0	35.0	37.0
5	35.74534217221298	37.0	35.0	37.0	35.0	37.0
6	35.81203854601068	37.0	35.0	37.0	35.0	37.0
7	35.78944718020013	37.0	35.0	37.0	35.0	37.0
8	35.78110213120101	37.0	35.0	37.0	35.0	37.0
9	37.48516516946423	39.0	37.0	39.0	35.0	39.0
10	37.41017264245435	39.0	37.0	39.0	34.0	39.0
11	37.48956881477117	39.0	37.0	39.0	35.0	39.0
12	37.382362029447364	39.0	37.0	39.0	34.0	39.0
13	37.40627106110411	39.0	37.0	39.0	34.0	39.0
14	38.625283860206444	40.0	38.0	41.0	34.0	41.0
15	38.61685866244314	40.0	38.0	41.0	34.0	41.0
16	38.591446967478255	40.0	38.0	41.0	34.0	41.0
17	38.583727986184165	40.0	38.0	41.0	34.0	41.0
18	38.5066294117736	40.0	38.0	41.0	34.0	41.0
19	38.53327307893746	40.0	38.0	41.0	34.0	41.0
20	38.49313266233527	40.0	38.0	41.0	34.0	41.0
21	38.461332253929434	40.0	38.0	41.0	34.0	41.0
22	38.414610061139896	40.0	38.0	41.0	34.0	41.0
23	38.35825035751893	40.0	38.0	41.0	34.0	41.0
24	38.328256572828764	40.0	38.0	41.0	34.0	41.0
25	38.289558833981665	40.0	38.0	41.0	34.0	41.0
26	38.092347506894555	40.0	37.0	41.0	33.0	41.0
27	37.98377163953623	40.0	37.0	41.0	33.0	41.0
28	37.88472893839181	40.0	37.0	41.0	33.0	41.0
29	37.79223121557447	40.0	37.0	41.0	33.0	41.0
30	37.719620467877235	40.0	37.0	41.0	33.0	41.0
31	37.59580222107852	40.0	37.0	41.0	33.0	41.0
32	37.47464602222691	40.0	36.0	41.0	32.0	41.0
33	37.382306580616806	40.0	36.0	41.0	32.0	41.0
34	37.251245708386534	39.0	36.0	41.0	31.0	41.0
35	37.12455117952265	39.0	36.0	41.0	31.0	41.0
36	36.9957112849964	39.0	35.0	41.0	31.0	41.0
37	36.891196792436375	39.0	35.0	41.0	31.0	41.0
38	36.76986416548754	39.0	35.0	41.0	30.0	41.0
39	36.642154418944145	39.0	35.0	41.0	30.0	41.0
40	36.48207062063876	39.0	35.0	41.0	30.0	41.0
41	36.28791906083795	39.0	35.0	41.0	30.0	41.0
42	36.19845539721274	39.0	35.0	40.0	29.0	41.0
43	35.01134029400986	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	12.0
12	7.0
13	6.0
14	5.0
15	7.0
16	13.0
17	39.0
18	103.0
19	210.0
20	409.0
21	757.0
22	1356.0
23	2228.0
24	3866.0
25	5786.0
26	8844.0
27	12929.0
28	17868.0
29	24434.0
30	32849.0
31	42656.0
32	55442.0
33	71984.0
34	97091.0
35	126716.0
36	173186.0
37	264700.0
38	449638.0
39	590662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60864013759375	17.79902420139822	12.239674041529158	26.352661619478873
2	19.289287134711923	20.044752247063858	34.384122277777465	26.281838340446743
3	21.078469672766207	22.191730966306768	28.677731900871606	28.05206746005542
4	15.622052705625686	14.87379594124642	31.92335358559863	37.58079776752927
5	15.305238251022905	36.559127860466546	32.196363464059836	15.939270424450717
6	36.47156911621117	32.180333711225515	15.247621875269369	16.100475297293944
7	30.440853488563175	29.666939038043445	20.260498605965992	19.631708867427392
8	28.516325395917253	33.04447853147301	18.807285573071226	19.631910499538517
9	27.750173781675773	13.740925924899095	18.977513482887232	39.5313868105379
10	19.197292484011832	26.42771917284459	30.492924981260817	23.88206336188276
11	35.83814183911673	21.70977981269385	19.894284284137957	22.557794064051464
12	23.22217187020336	25.047496964176524	27.56547876788666	24.16485239773345
13	30.5857261604054	18.703797892037095	24.695346482099353	26.015129465458152
14	23.337404621710437	20.19668204279541	24.969666969282862	31.496246366211295
15	26.67083709083174	26.042803472709853	21.902288070789	25.38407136566941
16	24.89622247280613	25.125326959070193	23.308470413764216	26.669980154359465
17	23.69197468912109	26.41204227620474	25.428228798005453	24.467754236668714
18	23.456720423467758	23.65200112309086	26.809257535118014	26.082020918323366
19	24.53202447208933	24.19242558892959	26.581060393354004	24.69448954562708
20	24.275901282934715	24.803471701689325	26.625570681884515	24.295056333491445
21	26.807342030062344	23.808820497517154	25.428480838144356	23.95535663427615
22	25.85150500728144	24.497797421226114	25.620737056100605	24.029960515391842
23	24.966491263532664	25.04023820817608	25.308812180192568	24.684458348098683
24	25.126083079486904	24.877168238304957	25.845909716197763	24.150838966010372
25	24.785123179577088	24.62900951753973	26.24393150355553	24.341935799327658
26	25.487861494870227	24.791524999105256	26.241209470055367	23.479404035969154
27	25.53731177012326	24.653860675235695	25.1798684451291	24.628959109511943
28	24.208152893597223	23.962262534082125	27.041588135160055	24.787996437160597
29	24.12593740028662	24.171153401206062	27.17083431839021	24.532074880117108
30	23.742231492818618	25.104054771346668	26.778760678310586	24.37495305752413
31	23.874855013910096	25.077388924650585	26.534836231878945	24.512919829560374
32	23.378436756324064	24.890123101444644	26.77946639069952	24.951973751531774
33	24.358973712717592	25.234057074993537	26.300338086642327	24.106631125646548
34	24.786030524077145	25.31919623391543	26.091043955296144	23.803729286711285
35	24.897734713639554	25.01634480300795	26.532013382323218	23.55390710102928
36	24.449103266389795	25.397227860920218	25.03040864275881	25.123260229931176
37	23.45893837669012	24.858214819859352	26.548799255574245	25.134047547876285
38	24.668882267514395	24.52768938170017	26.797109200422824	24.00631915036261
39	23.01570058841291	24.752710817713986	27.24029658067225	24.991292013200855
40	23.805493567683612	23.773081205820514	28.237468186233468	24.183957040262406
41	22.39336307743026	24.063280221754997	28.49545647241597	25.047900228398774
42	22.480417741407827	24.9129075300016	27.372869693735947	25.23380503485463
43	22.4962962701588	23.125993353197458	28.788276705795056	25.58943367084868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	960.0
1	796.5
2	633.0
3	1422.0
4	2211.0
5	2211.0
6	3014.0
7	3817.0
8	3564.5
9	3312.0
10	4534.0
11	5756.0
12	5756.0
13	10843.5
14	15931.0
15	21810.5
16	27690.0
17	24421.5
18	21153.0
19	21153.0
20	24523.0
21	27893.0
22	22528.5
23	17164.0
24	19112.0
25	21060.0
26	21060.0
27	24061.0
28	27062.0
29	31428.5
30	35795.0
31	40958.0
32	46121.0
33	46121.0
34	53132.0
35	60143.0
36	67260.5
37	74378.0
38	79900.0
39	85422.0
40	85422.0
41	91749.5
42	98077.0
43	102080.0
44	106083.0
45	110179.5
46	114276.0
47	114276.0
48	117142.5
49	120009.0
50	126595.0
51	133181.0
52	139622.5
53	146064.0
54	146064.0
55	147982.0
56	149900.0
57	140786.5
58	131673.0
59	118617.0
60	105561.0
61	105561.0
62	101561.5
63	97562.0
64	85059.0
65	72556.0
66	61203.0
67	49850.0
68	49850.0
69	43342.0
70	36834.0
71	34770.5
72	32707.0
73	37920.5
74	43134.0
75	43134.0
76	41780.0
77	40426.0
78	30804.0
79	21182.0
80	12903.5
81	4625.0
82	4625.0
83	3115.5
84	1606.0
85	1243.0
86	880.0
87	737.0
88	594.0
89	594.0
90	507.0
91	420.0
92	248.5
93	77.0
94	50.5
95	24.0
96	24.0
97	15.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1983811.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.91543006602106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.68079271917638	37.50408725834505
2	10.653230589732864	9.782953282401513
3	3.0394188159945936	4.186686662614449
4	1.2636591878773802	2.320858202730753
5	0.6918628709539992	1.5883590633282454
6	0.45379065175584593	1.2501595761185793
7	0.30143706511474005	0.9688428737807739
8	0.21713008150707644	0.7975696858134098
9	0.17608818077993976	0.7276648095048778
>10	1.2341133521410288	11.333225443235435
>50	0.15162710991963965	4.878844391049593
>100	0.11341858393990457	9.895950856600267
>500	0.013200445693863335	4.270508760811843
>1k	0.009570323128050917	7.700654129992847
>5k	3.3001114234658337E-4	1.0360340118482854
>10k+	3.3001114234658337E-4	1.7576009918241338
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12153	0.6126087616209407	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11576	0.5835233295913774	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	11069	0.5579664595064752	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8214	0.4140515401920848	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	6367	0.32094791288081376	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5931	0.29897001276835344	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	4069	0.20511026504036928	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	4063	0.20480781687368405	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	3807	0.19190336176178072	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	3593	0.18111604381667407	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	3413	0.17204259881611705	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	3290	0.16584241139906977	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	3231	0.16286833775999832	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	3214	0.16201140128772348	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	2911	0.14673776887011916	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	2811	0.14169696609203195	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	2809	0.1415961500364702	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2714	0.13680738739728734	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2645	0.13332923348040718	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	2591	0.13060719998024006	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	2470	0.12450782861875452	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2421	0.12203783525749176	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	2332	0.11755152078499413	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	2222	0.11200663772909819	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	2183	0.11004072464564417	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	2136	0.10767154733994316	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2099	0.10580645031205091	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	2044	0.10303400878410292	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	2003	0.10096727964508714	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.040802778087227E-5	0.0
5	0.0	0.0	0.0	1.0081605556174454E-4	0.0
6	0.0	0.0	0.0	1.0081605556174454E-4	0.0
7	0.0	0.0	0.0	1.0081605556174454E-4	0.0
8	0.0	0.0	0.0	1.0081605556174454E-4	0.0
9	0.0	0.0	0.0	1.0081605556174454E-4	0.0
10	0.0	0.0	0.0	1.512240833426168E-4	0.0
11	0.0	0.0	0.0	5.040802778087227E-4	0.0
12	0.0	0.0	0.0	7.057123889322118E-4	0.0
13	0.0	0.0	0.0	8.569364722748286E-4	0.0
14	0.0	0.0	0.0	0.0013610167500835513	0.0
15	0.0	0.0	0.0	0.0021675451945775076	0.0
16	0.0	0.0	0.0	0.0034781539168801867	5.040802778087227E-5
17	0.0	0.0	0.0	0.005393658972553333	5.040802778087227E-5
18	0.0	0.0	0.0	0.006401819528170778	2.0163211112348908E-4
19	0.0	0.0	0.0	0.007510796139349968	2.0163211112348908E-4
20	0.0	0.0	0.0	0.008770996833871775	2.5204013890436135E-4
21	0.0	0.0	0.0	0.010787317945106666	3.024481666852336E-4
22	0.0	0.0	0.0	0.015021592278699937	3.024481666852336E-4
23	0.0	0.0	0.0	0.021373003779089843	3.024481666852336E-4
24	0.0	0.0	0.0	0.030144000612961616	3.024481666852336E-4
25	0.0	0.0	0.0	0.03518480339104885	3.024481666852336E-4
26	0.0	0.0	0.0	0.044459880502729344	3.024481666852336E-4
27	0.0	0.0	0.0	0.06840369369864367	3.024481666852336E-4
28	0.0	0.0	0.0	0.12647374170220851	3.024481666852336E-4
29	0.0	0.0	0.0	0.2021361914012978	3.024481666852336E-4
30	0.0	0.0	0.0	0.31076549126907754	3.024481666852336E-4
31	0.0	0.0	0.0	0.5504556633671251	3.024481666852336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATCA	2640	0.0	18.99053	14
CCAGGAC	2805	0.0	18.796791	3
CAGGACA	2825	0.0	18.729204	4
GACAGGC	2815	0.0	18.40142	7
GCATCAG	2760	0.0	18.298914	15
TCCAGGA	3010	0.0	17.885384	2
CGGACCA	270	0.0	17.814816	9
GCTGCAT	2815	0.0	17.54707	12
ACAGGCT	2990	0.0	17.386288	8
GGACAGG	3140	0.0	17.321655	6
CTGCATC	2865	0.0	17.30541	13
ACGGACC	290	0.0	17.224138	8
CGCGCTA	195	0.0	17.076923	24
AAGACGG	285	0.0	16.877193	5
AGGCCAT	2955	0.0	16.840948	25
ATCAAGC	2955	0.0	16.65313	30
GGCCATC	3000	0.0	16.526667	26
GACGGAC	280	0.0	16.517857	7
ATACCGT	180	1.0913936E-11	16.444445	6
CGACGGT	160	6.311893E-10	16.1875	7
>>END_MODULE