FastQCFastQC Report
Fri 10 Feb 2017
ERR1630131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486092
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.7877109682940678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27450.5647079153740444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23270.4787159632332974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.21271693424290053No Hit
GCTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTA6870.14133127062366796No Hit
GTTCTAAGCTGGTTGTTAAATGTATACCGGCCGTCCCGAGGGA5390.11088435933938431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTC303.5964488E-430.83333434
CACAGGA502.7153146E-729.59999833
TATATGC358.8620017E-426.428573
TAAGATA358.8620017E-426.428574
TATACTG451.3216778E-424.6666665
GATAGAG451.3216778E-424.6666667
TCTTATA3300.024.1060637
CAGTATT551.8997316E-523.54545414
TAAGTCT400.001929592723.12535
ATGACAG400.001929592723.1255
CACGTGC802.7175702E-823.1258
AGCCAGG502.6989946E-422.19999935
GGCCAGC502.6989946E-422.19999931
CGCCGTT502.6989946E-422.19999925
TAATACT705.0919316E-621.1428574
CACGTGT2100.021.1428577
GCCGGGT450.003822679920.5555558
GTTACTT450.003822679920.55555528
ATAAGCA450.003822679920.5555554
ACTTATA555.1372277E-420.18181831
Produced by FastQC (version 0.11.5)