FastQCFastQC Report
Fri 10 Feb 2017
ERR1630130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486982
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT228274.687442246325326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT180093.6980832967132256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT141142.898259073230633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75831.5571417424052634No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA39490.8109129290199638No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA28400.583183772706178No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23980.4924206644188081No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.47455552771971043No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.44868188146584476No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.34559798924806256No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA16480.33841086528865544No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.3343039373118513No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA12310.25278141697229056No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA12110.2486744889954865No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA12050.24744241060244526No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA11550.23717509066043507No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.23696974426159487No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.18789195493878624No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA9020.1852224517538636No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA8850.18173156297358015No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.18152621657473994No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7400.15195633514175064No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7110.14600128957538472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6950.14271574719394145No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA6560.13470723763917353No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA6400.13142169525773026No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6290.12916288487048802No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6230.12793080647744678No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6140.12608268888788496No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6100.12526130329252416No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6080.12485061049484375No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5950.12218110730992111No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5910.1213597217145603No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA5660.1162260617435552No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA5620.11540467614819439No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT5580.11458329055283357No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5500.11294051936211195No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5450.11191378736791094No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA5440.11170844096907073No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5370.1102710161771893No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5370.1102710161771893No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5250.10780685939110685No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA5230.10739616659342646No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA5170.10616408820038523No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5120.10513735620618421No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5040.10349458501546258No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.10164646742590075No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4950.10164646742590075No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4930.10123577462822035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAC303.5964584E-430.83333430
ACGTTTA551.8511855E-830.27272826
GCCCAAG250.00549327229.59999822
AGTATAC250.00549327229.59999837
ATTAGGT405.9332073E-527.7519
AGAGACA405.9332073E-527.7520
TTAGGTC358.8620244E-426.42857220
GAGTAGT358.8620244E-426.4285721
AGGTCGT358.8620244E-426.42857222
TTGATCA659.368523E-825.61538520
TCACGTT659.368523E-825.61538524
TATTGTA451.3216826E-424.66666631
TAAGAGA451.3216826E-424.66666618
ATAAGAG551.89974E-523.54545417
TACTATA551.89974E-523.5454542
ATGCCCC802.7175702E-823.12537
ACCTATA652.677576E-622.7692329
TATAAGC652.677576E-622.769232
TGATCAC753.7316022E-722.221
AGCGTAT502.6990045E-422.1999997
Produced by FastQC (version 0.11.5)