##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486982
Sequences flagged as poor quality	0
Sequence length	43
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.672242505883176	31.0	31.0	34.0	30.0	34.0
2	31.726277357273986	31.0	31.0	34.0	30.0	34.0
3	31.739331227848258	33.0	31.0	34.0	28.0	34.0
4	35.50136760701628	37.0	35.0	37.0	33.0	37.0
5	35.49769190647704	37.0	35.0	37.0	33.0	37.0
6	35.63952055722799	37.0	35.0	37.0	33.0	37.0
7	35.58239318907064	37.0	35.0	37.0	33.0	37.0
8	35.670343051693905	37.0	35.0	37.0	33.0	37.0
9	37.22416023590194	39.0	37.0	39.0	34.0	39.0
10	37.12028165312065	39.0	37.0	39.0	33.0	39.0
11	37.25945312147061	39.0	37.0	39.0	34.0	39.0
12	37.1740084849132	39.0	37.0	39.0	34.0	39.0
13	37.262050753415934	39.0	37.0	39.0	34.0	39.0
14	38.15172018678309	40.0	37.0	41.0	33.0	41.0
15	38.11580920855391	40.0	37.0	41.0	33.0	41.0
16	37.99469384905397	40.0	37.0	41.0	33.0	41.0
17	38.224098631982294	40.0	38.0	41.0	33.0	41.0
18	38.370157418549354	40.0	38.0	41.0	34.0	41.0
19	38.44430389624257	40.0	38.0	41.0	34.0	41.0
20	38.51286495188734	40.0	38.0	41.0	34.0	41.0
21	38.51072113548345	40.0	38.0	41.0	34.0	41.0
22	38.463834392236265	40.0	38.0	41.0	34.0	41.0
23	38.38169583270018	40.0	38.0	41.0	34.0	41.0
24	38.32972881954569	40.0	38.0	41.0	34.0	41.0
25	38.28020337507341	40.0	37.0	41.0	34.0	41.0
26	37.962175193333636	40.0	37.0	41.0	33.0	41.0
27	37.756284626536505	40.0	36.0	41.0	33.0	41.0
28	37.58182848647383	39.0	36.0	41.0	33.0	41.0
29	37.350735756147046	39.0	35.0	41.0	33.0	41.0
30	37.02564776521514	39.0	35.0	40.0	33.0	41.0
31	36.609412668230036	38.0	35.0	40.0	32.0	41.0
32	36.25429071300377	38.0	35.0	40.0	31.0	41.0
33	35.989899010641054	37.0	35.0	40.0	31.0	41.0
34	35.752656155669	37.0	35.0	40.0	30.0	41.0
35	35.49564665634459	37.0	35.0	40.0	30.0	41.0
36	35.147091678953224	37.0	35.0	40.0	28.0	41.0
37	34.83448874907081	36.0	34.0	40.0	26.0	41.0
38	34.48453536270335	35.0	34.0	40.0	24.0	41.0
39	34.08434603332361	35.0	33.0	40.0	22.0	41.0
40	33.6190331470157	35.0	33.0	40.0	20.0	41.0
41	33.09149824839522	35.0	33.0	40.0	16.0	41.0
42	32.582561573117694	35.0	33.0	40.0	12.0	41.0
43	31.544705964491502	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	9.0
12	9.0
13	4.0
14	4.0
15	6.0
16	6.0
17	3.0
18	11.0
19	46.0
20	116.0
21	227.0
22	421.0
23	856.0
24	1334.0
25	2194.0
26	3416.0
27	4933.0
28	7409.0
29	9512.0
30	12154.0
31	14904.0
32	18401.0
33	23678.0
34	30389.0
35	38617.0
36	54370.0
37	91788.0
38	91153.0
39	81004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.80717973148905	18.959633004915993	14.998500971288466	16.234686292306492
2	17.421999170400547	29.267200841098852	32.30591685113618	21.00488313736442
3	22.24517538635925	30.391677721147804	31.423132682522148	15.940014209970798
4	13.450189124033333	18.063090627579665	31.136879802538903	37.3498404458481
5	11.689138407579748	44.799397102973	28.757325732778625	14.754138756668626
6	27.8260798140383	33.465507965386806	19.233154408171142	19.475257812403743
7	23.768024280158198	36.7650139019512	20.910629140296766	18.556332677593833
8	34.23494092184106	29.80048543888686	19.52002332735091	16.44455031192118
9	30.535625546734785	11.13716728749728	22.215605504926263	36.11160166084167
10	21.315982931607326	32.71229737444094	25.11098972857313	20.860729965378596
11	29.32634060396483	28.693873695537004	17.256489972935345	24.723295727562824
12	20.190684665963012	35.292680222266945	27.367335959029287	17.149299152740756
13	32.792998509185146	21.049032613115067	27.084984660624006	19.072984217075785
14	23.565142038104078	25.026387012250968	31.30403177119483	20.10443917845013
15	28.114386158009946	27.450090557761886	27.515596058991914	16.91992722523625
16	18.056519542816776	27.545781979621424	31.512458366017636	22.885240111544164
17	17.64336258835029	31.034206603118804	31.45906830231918	19.86336250621173
18	15.794012920395414	24.718572760389502	36.40257750799824	23.084836811216842
19	16.639013351622854	29.003125372190347	39.2488839423223	15.108977333864496
20	17.970274055303893	26.083304927081496	40.1534348292134	15.792986188401215
21	19.937081863395363	26.093161554225823	37.917828585040105	16.051927997338712
22	19.92352900107191	27.454402832137532	35.95512770492544	16.66694046186512
23	17.82920107930067	28.937209178162643	36.478350329170276	16.755239413366407
24	16.407793306528784	30.255327712317907	37.054963017113565	16.28191596403974
25	16.34290384449528	30.25471167312139	36.79786932576564	16.6045151566177
26	17.4712823061222	29.975851263496395	36.62743181472827	15.925434615653145
27	15.895248695023637	31.154539592839157	36.79745863296795	16.15275307916925
28	14.988849690542978	32.60757071103244	37.325198877987276	15.078380720437307
29	15.58722909676333	32.4387759711858	36.77610260748857	15.197892324562304
30	14.338722991814892	34.48176729324698	37.59030107888998	13.58920863604815
31	15.271611681745936	33.458115495028565	36.24671959127853	15.02355323194697
32	13.334373755087455	34.30475869744672	36.75105034683007	15.609817200635753
33	14.020230727213736	34.04294203892546	37.63753075062323	14.299296483237573
34	13.378523230838102	34.60661790374182	36.068273570686394	15.946585294733687
35	13.94425255964286	35.35613225950856	35.80830502975469	14.891310151093881
36	13.332320291099057	35.7327375549815	35.35695364510393	15.57798850881552
37	13.746503977559746	36.18039270445314	34.89369216932043	15.179411148666686
38	13.688185600289128	35.035586530919005	35.05550513160651	16.22072273718536
39	13.994767773757552	34.387718642578164	36.06930030268059	15.548213280983692
40	13.603172191169282	35.111564698489886	35.47831336681849	15.806949743522347
41	13.078101449334884	34.802929061033055	35.83890164318188	16.280067846450176
42	12.98507953066027	35.0891819410163	35.49823196750598	16.42750656081744
43	12.901298199933468	35.691462928814616	34.951394507394525	16.45584436385739
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	158.0
2	264.0
3	1038.5
4	1813.0
5	1813.0
6	2800.0
7	3787.0
8	3577.5
9	3368.0
10	4704.0
11	6040.0
12	6040.0
13	12588.5
14	19137.0
15	31768.0
16	44399.0
17	36594.0
18	28789.0
19	28789.0
20	31694.0
21	34599.0
22	23545.0
23	12491.0
24	12868.5
25	13246.0
26	13246.0
27	13324.0
28	13402.0
29	13104.0
30	12806.0
31	12491.0
32	12176.0
33	12176.0
34	12519.5
35	12863.0
36	12688.0
37	12513.0
38	13797.5
39	15082.0
40	15082.0
41	17263.0
42	19444.0
43	22074.5
44	24705.0
45	28691.5
46	32678.0
47	32678.0
48	37476.5
49	42275.0
50	36098.5
51	29922.0
52	26895.5
53	23869.0
54	23869.0
55	22203.5
56	20538.0
57	18556.0
58	16574.0
59	13922.5
60	11271.0
61	11271.0
62	9301.5
63	7332.0
64	5863.5
65	4395.0
66	3610.0
67	2825.0
68	2825.0
69	2439.5
70	2054.0
71	1558.5
72	1063.0
73	826.0
74	589.0
75	589.0
76	467.5
77	346.0
78	255.0
79	164.0
80	110.0
81	56.0
82	56.0
83	43.0
84	30.0
85	24.0
86	18.0
87	10.5
88	3.0
89	3.0
90	2.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	486982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.064641996885335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.42938389637526	18.87845961313993
2	11.566375485599032	6.029468724673792
3	4.768822835017292	3.728929798238925
4	2.5307823167603614	2.638557402336275
5	1.5617105150238477	2.0352712738434007
6	1.0687737380708044	1.671432291509305
7	0.792641420308525	1.4461940396571096
8	0.5782741462253395	1.2058006877934395
9	0.46244955603384164	1.0848223907676584
>10	3.495517231804245	18.259043918346375
>50	0.43881052255948716	7.890395650587992
>100	0.27021904710575245	13.486937925526203
>500	0.02284650835587994	3.753204905570377
>1k	0.010241538228497904	5.050953838889383
>5k	7.878106329613772E-4	1.5570933799617117
>10k+	0.002363431898884132	11.283434159158125
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22827	4.687442246325326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18009	3.6980832967132256	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14114	2.898259073230633	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7583	1.5571417424052634	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3949	0.8109129290199638	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	2840	0.583183772706178	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2398	0.4924206644188081	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2311	0.47455552771971043	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2185	0.44868188146584476	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1683	0.34559798924806256	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1648	0.33841086528865544	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1628	0.3343039373118513	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1231	0.25278141697229056	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1211	0.2486744889954865	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1205	0.24744241060244526	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	1155	0.23717509066043507	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1154	0.23696974426159487	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	915	0.18789195493878624	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	902	0.1852224517538636	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	885	0.18173156297358015	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	884	0.18152621657473994	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	740	0.15195633514175064	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	711	0.14600128957538472	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	695	0.14271574719394145	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	656	0.13470723763917353	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	640	0.13142169525773026	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	629	0.12916288487048802	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	623	0.12793080647744678	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	614	0.12608268888788496	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	610	0.12526130329252416	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	608	0.12485061049484375	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	595	0.12218110730992111	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	591	0.1213597217145603	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	566	0.1162260617435552	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	562	0.11540467614819439	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	558	0.11458329055283357	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	550	0.11294051936211195	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	545	0.11191378736791094	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	544	0.11170844096907073	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	537	0.1102710161771893	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	537	0.1102710161771893	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	525	0.10780685939110685	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	523	0.10739616659342646	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	517	0.10616408820038523	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	512	0.10513735620618421	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	504	0.10349458501546258	No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	495	0.10164646742590075	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	495	0.10164646742590075	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	493	0.10123577462822035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	4.1069279768040707E-4	0.0
12	0.0	0.0	0.0	6.160391965206106E-4	0.0
13	0.0	0.0	0.0	8.213855953608141E-4	0.0
14	0.0	0.0	0.0	0.0010267319942010176	0.0
15	0.0	0.0	0.0	0.0016427711907216283	0.0
16	0.0	0.0	0.0	0.005544352768685495	0.0
17	0.0	0.0	0.0	0.011910091132731806	0.0
18	0.0	0.0	0.0	0.014168901519974044	0.0
19	0.0	0.0	0.0	0.016427711907216282	0.0
20	0.0	0.0	0.0	0.020534639884020354	0.0
21	0.0	0.0	0.0	0.028132456641107886	0.0
22	0.0	0.0	0.0	0.04168531896456132	0.0
23	0.0	0.0	0.0	0.0638627300393033	0.0
24	0.0	0.0	0.0	0.09815557864561729	0.0
25	0.0	0.0	0.0	0.1102710161771893	0.0
26	0.0	0.0	0.0	0.12649338168556537	0.0
27	0.0	0.0	0.0	0.1443585183846631	0.0
28	0.0	0.0	0.0	0.17331236062113178	0.0
29	0.0	0.0	0.0	0.22382757473582185	0.0
30	0.0	0.0	0.0	0.34518729645038215	0.0
31	0.0	0.0	0.0	0.6877050897158417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGAC	30	3.5964584E-4	30.833334	30
ACGTTTA	55	1.8511855E-8	30.272728	26
GCCCAAG	25	0.005493272	29.599998	22
AGTATAC	25	0.005493272	29.599998	37
ATTAGGT	40	5.9332073E-5	27.75	19
AGAGACA	40	5.9332073E-5	27.75	20
TTAGGTC	35	8.8620244E-4	26.428572	20
GAGTAGT	35	8.8620244E-4	26.428572	1
AGGTCGT	35	8.8620244E-4	26.428572	22
TTGATCA	65	9.368523E-8	25.615385	20
TCACGTT	65	9.368523E-8	25.615385	24
TATTGTA	45	1.3216826E-4	24.666666	31
TAAGAGA	45	1.3216826E-4	24.666666	18
ATAAGAG	55	1.89974E-5	23.545454	17
TACTATA	55	1.89974E-5	23.545454	2
ATGCCCC	80	2.7175702E-8	23.125	37
ACCTATA	65	2.677576E-6	22.76923	29
TATAAGC	65	2.677576E-6	22.76923	2
TGATCAC	75	3.7316022E-7	22.2	21
AGCGTAT	50	2.6990045E-4	22.199999	7
>>END_MODULE