FastQCFastQC Report
Fri 10 Feb 2017
ERR1630129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506928
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113550.45294479937198034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95250.379947090622467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85010.339100285289406No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC74830.29849281670634337No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT44250.17651085312382328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42650.1701285397905325No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG29990.11962848554086915No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG28500.1136849562492421No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG28380.1132062827492453No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT26860.10714308508261905No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT26800.10690374833262065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2750.018.83636529
CAGGACA23200.018.1810344
CCAGGAC22900.018.1768553
TCTAGCG2850.018.17543828
GACAGGC21200.018.1509447
TGCATCA21250.017.67294114
GCATCAG21450.017.42191115
GGTATCA38850.017.2857151
GCTGCAT21650.017.26097112
ACAGGCT23100.016.9783558
CTGCATC22600.016.78097313
GTACTAA1708.54925E-1116.323531
AAGACGG4250.016.1058835
GGACAGG25450.015.919456
ACCGCGT700.002593639515.8571428
CGCAATA3650.015.71232936
TCCAGGA26500.015.6377362
ATCAAGC23550.015.39702830
GGCCATC24700.015.27935226
GACGGAC4650.015.118287
Produced by FastQC (version 0.11.5)