FastQCFastQC Report
Fri 10 Feb 2017
ERR1630128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020764
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC80830.3999972287709005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74440.3683755252963731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63700.3152273100668856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53840.2664338834223096No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT36620.18121858861301965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.145934903828453No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT28890.14296572979328612No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG26290.13009930897422955No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG23920.11837107153532031No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG23110.11436268658784499No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT22080.10926560449414183No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG20330.10060551355823837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC555.144949E-420.18181825
GGTATCA24000.019.8104171
TGCATCA19200.018.69270914
CGATACG500.00703668418.49999826
GACAGGC19950.018.1754387
GACGGAC4050.017.8148167
TATCGCG1258.589268E-917.767
GTTCGCG1054.8017137E-717.6190530
GCATCAG20350.017.36363615
ATCAAGC20700.016.98067530
GTGTAGG4250.016.9764691
CAGGACA21950.016.772214
GCTGCAT20850.016.76978512
ACAGGCT21800.016.717898
CATCAGA21750.016.67126516
CGAATTA1452.9831426E-916.58620615
CTTATAC15550.016.53697837
TCTAGCG2600.016.36538528
CTGCATC21750.016.33103413
AGAGGCC21600.016.187523
Produced by FastQC (version 0.11.5)