##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630128.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2020764
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.956807920172768	33.0	31.0	34.0	30.0	34.0
2	32.15304953967905	33.0	31.0	34.0	30.0	34.0
3	32.294528208143056	34.0	31.0	34.0	30.0	34.0
4	35.934502989958254	37.0	35.0	37.0	35.0	37.0
5	35.856567120158516	37.0	35.0	37.0	35.0	37.0
6	35.91155275925343	37.0	35.0	37.0	35.0	37.0
7	35.89655100744075	37.0	35.0	37.0	35.0	37.0
8	35.88336193637654	37.0	35.0	37.0	35.0	37.0
9	37.607837926645566	39.0	37.0	39.0	35.0	39.0
10	37.53239814248472	39.0	37.0	39.0	35.0	39.0
11	37.600472395588994	39.0	37.0	39.0	35.0	39.0
12	37.49291505588975	39.0	37.0	39.0	35.0	39.0
13	37.52608666821064	39.0	37.0	39.0	35.0	39.0
14	38.78567660548189	40.0	38.0	41.0	35.0	41.0
15	38.774577832938434	40.0	38.0	41.0	35.0	41.0
16	38.762230027850855	40.0	38.0	41.0	35.0	41.0
17	38.7432906564052	40.0	38.0	41.0	35.0	41.0
18	38.694184971624594	40.0	38.0	41.0	35.0	41.0
19	38.69773758835767	40.0	38.0	41.0	34.0	41.0
20	38.64729429067422	40.0	38.0	41.0	34.0	41.0
21	38.61103968598015	40.0	38.0	41.0	34.0	41.0
22	38.57167982010764	40.0	38.0	41.0	34.0	41.0
23	38.519757378892336	40.0	38.0	41.0	34.0	41.0
24	38.50560827489009	40.0	38.0	41.0	34.0	41.0
25	38.4886834880273	40.0	38.0	41.0	34.0	41.0
26	38.3347659598053	40.0	38.0	41.0	34.0	41.0
27	38.2482323517244	40.0	38.0	41.0	34.0	41.0
28	38.159806390058414	40.0	38.0	41.0	34.0	41.0
29	38.09089136583985	40.0	38.0	41.0	33.0	41.0
30	38.02771179613255	40.0	37.0	41.0	33.0	41.0
31	37.937640417188746	40.0	37.0	41.0	33.0	41.0
32	37.84963706796044	40.0	37.0	41.0	33.0	41.0
33	37.775693252650974	40.0	37.0	41.0	33.0	41.0
34	37.68136061410437	40.0	37.0	41.0	33.0	41.0
35	37.582368846634246	40.0	37.0	41.0	33.0	41.0
36	37.48116158047154	40.0	37.0	41.0	32.0	41.0
37	37.410671904289664	40.0	36.0	41.0	32.0	41.0
38	37.312364531434646	40.0	36.0	41.0	32.0	41.0
39	37.2294211496246	39.0	36.0	41.0	31.0	41.0
40	37.108061109560545	39.0	36.0	41.0	31.0	41.0
41	36.976154563323576	39.0	36.0	41.0	31.0	41.0
42	36.912968560405865	39.0	36.0	41.0	31.0	41.0
43	35.798801344442005	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	3.0
12	2.0
13	3.0
14	5.0
15	5.0
16	21.0
17	35.0
18	89.0
19	156.0
20	280.0
21	550.0
22	985.0
23	1771.0
24	2795.0
25	4305.0
26	6545.0
27	9745.0
28	14079.0
29	19713.0
30	26734.0
31	36013.0
32	48067.0
33	63353.0
34	87193.0
35	120933.0
36	170771.0
37	266904.0
38	484965.0
39	654740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.561395590974506	17.93277196149575	12.074888507514979	26.430943940014767
2	18.664326957526956	20.005156465574405	34.70053900405985	26.629977572838786
3	19.60273441134145	22.122672414987598	29.839704191088124	28.434888982582823
4	14.259309845187266	15.109730775092986	34.370663768752806	36.26029561096694
5	14.644362231314492	36.71492564198491	33.565869146520825	15.074842980179772
6	35.19564877442393	34.61403706716866	15.717768131261245	14.47254602714617
7	30.488567690239933	30.533402218170952	20.45162126799567	18.526408823593453
8	28.497736499660526	33.70373779422041	18.986729771512163	18.811795934606913
9	27.443630230942357	14.205468822682906	18.833421418829708	39.51747952754503
10	17.89585523099184	26.24452929684021	31.500660146360488	24.358955325807464
11	36.657521610638355	21.518396012597215	20.972166962594347	20.851915414170087
12	23.001251011993485	24.694818395418764	28.43716534934312	23.866765243244632
13	30.010085294472784	18.50418950456362	25.24525377530479	26.24047142565881
14	22.76035202527361	19.79850195272679	25.352243013038635	32.08890300896097
15	25.647675829537743	26.744835121765824	21.8857818132152	25.721707235481233
16	24.95694697649008	25.875213533099362	23.181826279565552	25.986013210845005
17	23.861420729981333	26.527046206286336	25.230259446427194	24.381273617305137
18	24.118006852853675	24.337478300286428	26.499135970355763	25.04537887650413
19	24.71941305367673	24.700360853617738	25.940782793042633	24.6394432996629
20	24.73633734567718	24.967537030548844	25.71438327286116	24.581742350912823
21	26.65387942382188	24.326937732461584	25.040776656749625	23.97840618696691
22	25.756694002862286	24.784091561409447	25.353480168886623	24.10573426684165
23	24.75152961949045	24.76949312240321	25.392722752384746	25.0862545057216
24	24.991537853999773	24.982382900724676	25.519555969920287	24.50652327535526
25	24.934183308887135	24.44139939151727	25.915297382574114	24.709119917021482
26	25.257674820018565	25.255645884427867	25.796926311038792	23.68975298451477
27	25.562658479664123	24.75687413275375	24.964567856513675	24.715899531068448
28	24.321543732964365	24.446001611271775	26.350974186000936	24.88148046976292
29	24.1101880278944	24.554524922257126	26.601522988335102	24.73376406151337
30	23.92441670576079	25.267522580568535	26.238294031366355	24.569766682304316
31	24.24008939193295	24.96318224196393	25.878974486877244	24.917753879225877
32	23.868695206367494	24.64934054644679	26.1732691199962	25.308695127189516
33	24.246176198705044	24.898454247997293	26.15456332357465	24.700806229723014
34	25.094815624189664	24.818088604112106	25.669400286228377	24.41769548546985
35	25.145044151617903	24.652705610353312	26.504876373490422	23.697373864538363
36	24.61242381594288	25.002523797929893	25.11569881490367	25.269353571223558
37	24.0994990013678	24.338715456134413	26.368195395404907	25.19359014709288
38	24.564372682807097	23.8967044147659	26.875775696716687	24.663147205710313
39	23.54767800693203	24.124786466900638	26.99676953864974	25.330765987517594
40	23.932285016953983	23.470776399421208	28.067453695730922	24.529484887893886
41	22.69740553572807	23.687971480093665	28.327454368743705	25.287168615434556
42	23.138179421248598	24.25656830782813	27.658449972386684	24.946802298536593
43	22.854821245825836	22.745209237694258	28.65831932872913	25.74165018775077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	811.0
1	645.0
2	479.0
3	935.5
4	1392.0
5	1392.0
6	1853.5
7	2315.0
8	2065.0
9	1815.0
10	2476.5
11	3138.0
12	3138.0
13	5669.0
14	8200.0
15	11891.5
16	15583.0
17	14623.5
18	13664.0
19	13664.0
20	16281.5
21	18899.0
22	17793.5
23	16688.0
24	19132.0
25	21576.0
26	21576.0
27	24847.0
28	28118.0
29	33131.5
30	38145.0
31	44102.5
32	50060.0
33	50060.0
34	58558.0
35	67056.0
36	75884.0
37	84712.0
38	91724.5
39	98737.0
40	98737.0
41	105535.5
42	112334.0
43	115556.0
44	118778.0
45	123469.0
46	128160.0
47	128160.0
48	131685.0
49	135210.0
50	138748.5
51	142287.0
52	147303.5
53	152320.0
54	152320.0
55	152086.0
56	151852.0
57	142622.5
58	133393.0
59	121910.5
60	110428.0
61	110428.0
62	104254.0
63	98080.0
64	86038.5
65	73997.0
66	62574.5
67	51152.0
68	51152.0
69	43956.0
70	36760.0
71	32886.0
72	29012.0
73	29399.0
74	29786.0
75	29786.0
76	27396.5
77	25007.0
78	19014.0
79	13021.0
80	8285.5
81	3550.0
82	3550.0
83	2513.5
84	1477.0
85	1294.0
86	1111.0
87	997.5
88	884.0
89	884.0
90	769.0
91	654.0
92	370.5
93	87.0
94	56.5
95	26.0
96	26.0
97	16.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2020764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.430457458003104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64703593613	39.542033004767944
2	10.469462764207961	10.140817420202424
3	3.0510964703046897	4.432979934160642
4	1.3783654607552451	2.67019479234751
5	0.7406457591301239	1.7934906464500937
6	0.4700139256509537	1.365779365854453
7	0.31920821178547754	1.082157980478527
8	0.24663390977611452	0.9555674460090473
9	0.18445857706281238	0.804007194228389
>10	1.20504737193968	11.441152323504333
>50	0.14874927473622568	5.056408582801679
>100	0.1222952563099436	11.967418276416081
>500	0.012279818465624233	4.032182790865797
>1k	0.004297936462968482	3.363770188845062
>5k	4.093272821874745E-4	1.352040053067967
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	8083	0.3999972287709005	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7444	0.3683755252963731	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6370	0.3152273100668856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5384	0.2664338834223096	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3662	0.18121858861301965	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2949	0.145934903828453	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2889	0.14296572979328612	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2629	0.13009930897422955	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2392	0.11837107153532031	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2311	0.11436268658784499	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2208	0.10926560449414183	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2033	0.10060551355823837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.9486233919448285E-5	0.0
2	0.0	4.9486233919448285E-5	0.0	4.9486233919448285E-5	0.0
3	0.0	4.9486233919448285E-5	0.0	4.9486233919448285E-5	0.0
4	0.0	4.9486233919448285E-5	0.0	4.9486233919448285E-5	0.0
5	0.0	4.9486233919448285E-5	0.0	4.9486233919448285E-5	0.0
6	0.0	4.9486233919448285E-5	0.0	4.9486233919448285E-5	0.0
7	0.0	4.9486233919448285E-5	0.0	4.9486233919448285E-5	0.0
8	0.0	4.9486233919448285E-5	0.0	4.9486233919448285E-5	0.0
9	0.0	4.9486233919448285E-5	0.0	1.9794493567779314E-4	0.0
10	0.0	4.9486233919448285E-5	0.0	3.46403637436138E-4	0.0
11	0.0	4.9486233919448285E-5	0.0	4.453761052750346E-4	0.0
12	0.0	4.9486233919448285E-5	0.0	8.907522105500692E-4	0.0
13	0.0	4.9486233919448285E-5	0.0	9.897246783889659E-4	0.0
14	0.0	4.9486233919448285E-5	0.0	0.001039210912308414	0.0
15	0.0	4.9486233919448285E-5	0.0	0.0012866420819056554	9.897246783889657E-5
16	0.0	9.897246783889657E-5	0.0	0.0016330457193417934	1.4845870175834487E-4
17	0.0	9.897246783889657E-5	0.0	0.0019794493567779317	1.4845870175834487E-4
18	0.0	9.897246783889657E-5	0.0	0.0021279080585362765	1.4845870175834487E-4
19	0.0	9.897246783889657E-5	0.0	0.0023258529942140694	1.4845870175834487E-4
20	0.0	9.897246783889657E-5	0.0	0.002721742865569656	1.4845870175834487E-4
21	0.0	9.897246783889657E-5	0.0	0.0035135226082808283	2.4743116959724147E-4
22	0.0	9.897246783889657E-5	0.0	0.005393999497219863	2.4743116959724147E-4
23	0.0	9.897246783889657E-5	0.0	0.008808549637661795	2.4743116959724147E-4
24	0.0	9.897246783889657E-5	0.0	0.013559228093928831	2.4743116959724147E-4
25	0.0	9.897246783889657E-5	0.0	0.016825319532612418	2.4743116959724147E-4
26	0.0	9.897246783889657E-5	0.0	0.021872915392396143	2.4743116959724147E-4
27	0.0	9.897246783889657E-5	0.0	0.04983263755688443	2.4743116959724147E-4
28	0.0	9.897246783889657E-5	0.0	0.12114230063480941	2.4743116959724147E-4
29	0.0	1.4845870175834487E-4	0.0	0.2194219611988337	2.4743116959724147E-4
30	0.0	1.4845870175834487E-4	0.0	0.36060618657101967	2.4743116959724147E-4
31	0.0	1.4845870175834487E-4	0.0	0.649853223830195	2.4743116959724147E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAC	55	5.144949E-4	20.181818	25
GGTATCA	2400	0.0	19.810417	1
TGCATCA	1920	0.0	18.692709	14
CGATACG	50	0.007036684	18.499998	26
GACAGGC	1995	0.0	18.175438	7
GACGGAC	405	0.0	17.814816	7
TATCGCG	125	8.589268E-9	17.76	7
GTTCGCG	105	4.8017137E-7	17.61905	30
GCATCAG	2035	0.0	17.363636	15
ATCAAGC	2070	0.0	16.980675	30
GTGTAGG	425	0.0	16.976469	1
CAGGACA	2195	0.0	16.77221	4
GCTGCAT	2085	0.0	16.769785	12
ACAGGCT	2180	0.0	16.71789	8
CATCAGA	2175	0.0	16.671265	16
CGAATTA	145	2.9831426E-9	16.586206	15
CTTATAC	1555	0.0	16.536978	37
TCTAGCG	260	0.0	16.365385	28
CTGCATC	2175	0.0	16.331034	13
AGAGGCC	2160	0.0	16.1875	23
>>END_MODULE