FastQCFastQC Report
Fri 10 Feb 2017
ERR1630127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085596
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT332163.059701767508355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT253062.3310697533889218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT215391.9840714225181375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112421.0355601899785924No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA59940.5521391014705286No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35400.3260881580256375No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA35030.3226798919671775No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34290.3158633598502574No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28570.2631734088924425No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.23102516958426522No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.2194186419257256No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA21600.19896904557496525No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA17920.16507061558811933No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA17870.16461003909373284No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16210.14931889948010124No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA16040.14775293939918716No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA15080.1389098707069665No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.1369754494305432No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12910.11892085085059267No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA11830.1089723985718444No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11360.10464297952461138No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10870.10012932987962372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCC303.5993475E-430.83333434
CGAATTT303.5993475E-430.83333418
AATTCGT303.5993475E-430.83333430
TCTGGCA303.5993475E-430.83333424
TGGACGT250.0054962129.626
GTGGACG250.0054962129.625
GCGGGTT405.9398295E-527.7500026
CTAGATT358.869078E-426.4285733
TTCAGCA358.869078E-426.4285718
TCGTCTC358.869078E-426.4285733
CGGGTTA451.3231461E-424.6666667
ATCCCAG451.3231461E-424.66666618
AGACTAT851.9772415E-923.9411776
AATACTG851.9772415E-923.9411775
ACGGGAA551.9025327E-523.54545417
TAAGATA802.7253918E-823.1250024
ACGTTTT502.701968E-422.216
CTCTAAT957.1431714E-921.4210531
GATAGAG806.9593807E-720.81257
TGCGGAA450.003825673620.5555559
Produced by FastQC (version 0.11.5)