FastQCFastQC Report
Fri 10 Feb 2017
ERR1630126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507373
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67780.27032276410410416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63860.2546888715799364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53130.2118950790329161No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG52640.20994084246739514No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT37490.14951903845179795No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG30500.12164125560895807No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC28790.11482136881907877No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA28470.11354513269465692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26580.10600736308479033No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA25210.10054347717710926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.019.233821
TCGCCAT7150.018.88811113
ACCGTCG2550.018.8627458
ATACCGT3500.016.9142866
GTCGCCA8150.016.5705512
TCTAGCG2850.016.2280728
ATCTCGC8700.016.1609211
CGTCGTA3000.016.03333310
TACCGTC3250.015.9384627
GGTCCGA700.00259363915.8571436
CCGTCGT3050.015.7704939
TGTTCTA9150.015.77049115
CGGCCTT8800.015.76704524
AGGTCGC8600.015.70348810
TTGGCCG8950.015.50279331
TATACTG3750.015.2933345
GAGGTCG9400.015.1542569
CGCAATA3300.015.13636236
TTATACT3950.014.9873424
TAGAACA12100.014.8305784
Produced by FastQC (version 0.11.5)