##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630126.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2507373
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.848489235546527	33.0	31.0	34.0	30.0	34.0
2	32.05308863100943	33.0	31.0	34.0	30.0	34.0
3	32.200246233807256	34.0	31.0	34.0	30.0	34.0
4	35.84586577266326	37.0	35.0	37.0	35.0	37.0
5	35.76001815445887	37.0	35.0	37.0	35.0	37.0
6	35.820425999641856	37.0	35.0	37.0	35.0	37.0
7	35.7992496529236	37.0	35.0	37.0	35.0	37.0
8	35.78188566280326	37.0	35.0	37.0	35.0	37.0
9	37.479700866205384	39.0	37.0	39.0	35.0	39.0
10	37.40043942405059	39.0	37.0	39.0	34.0	39.0
11	37.471170823008784	39.0	37.0	39.0	35.0	39.0
12	37.36681179864344	39.0	37.0	39.0	34.0	39.0
13	37.39720855253686	39.0	37.0	39.0	34.0	39.0
14	38.63191994170791	40.0	38.0	41.0	34.0	41.0
15	38.63604537498011	40.0	38.0	41.0	34.0	41.0
16	38.617125573259344	40.0	38.0	41.0	34.0	41.0
17	38.59288586101869	40.0	38.0	41.0	34.0	41.0
18	38.565091033523935	40.0	38.0	41.0	34.0	41.0
19	38.591984918079596	40.0	38.0	41.0	34.0	41.0
20	38.54588088808486	40.0	38.0	41.0	34.0	41.0
21	38.50484511079923	40.0	38.0	41.0	34.0	41.0
22	38.485537652355674	40.0	38.0	41.0	34.0	41.0
23	38.43979854612776	40.0	38.0	41.0	34.0	41.0
24	38.43426406840945	40.0	38.0	41.0	34.0	41.0
25	38.40516827771536	40.0	38.0	41.0	34.0	41.0
26	38.26430531077745	40.0	38.0	41.0	34.0	41.0
27	38.166992705114076	40.0	38.0	41.0	33.0	41.0
28	38.10406748417567	40.0	38.0	41.0	33.0	41.0
29	38.04732363314114	40.0	38.0	41.0	33.0	41.0
30	37.98907741289389	40.0	37.0	41.0	33.0	41.0
31	37.88247620118746	40.0	37.0	41.0	33.0	41.0
32	37.79544447515388	40.0	37.0	41.0	33.0	41.0
33	37.74929019336174	40.0	37.0	41.0	33.0	41.0
34	37.66668381608959	40.0	37.0	41.0	33.0	41.0
35	37.59209180285502	40.0	37.0	41.0	32.0	41.0
36	37.495424095258265	40.0	37.0	41.0	32.0	41.0
37	37.448819541408476	40.0	37.0	41.0	32.0	41.0
38	37.349771653439674	40.0	37.0	41.0	31.0	41.0
39	37.26391366581677	40.0	36.0	41.0	31.0	41.0
40	37.18339712519837	39.0	36.0	41.0	31.0	41.0
41	37.06846288924703	39.0	36.0	41.0	31.0	41.0
42	37.019751349320586	39.0	36.0	41.0	31.0	41.0
43	35.9021717151776	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	11.0
11	8.0
12	5.0
13	2.0
14	3.0
15	14.0
16	23.0
17	44.0
18	86.0
19	170.0
20	341.0
21	633.0
22	1215.0
23	2184.0
24	3681.0
25	5669.0
26	8738.0
27	13164.0
28	18879.0
29	26671.0
30	37059.0
31	48304.0
32	63407.0
33	83543.0
34	110716.0
35	150009.0
36	210025.0
37	319385.0
38	591756.0
39	811622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.360550265158	19.501246922575938	12.717573332727122	25.420629479538942
2	17.08864217649309	21.26157536194256	36.5619714338473	25.087811027717056
3	18.925185841915024	23.505756821980615	31.28995965099728	26.279097685107082
4	13.85936595791691	15.769014023840889	36.020249081409105	34.35137093683309
5	13.27381287108061	37.97484458834007	35.20397643270467	13.547366107874655
6	33.39686596290221	36.48543714876087	16.208398192052	13.909298696284916
7	28.338743378029513	31.92377041628828	21.540353190370958	18.197133015311245
8	26.29756322653231	35.80093587990299	20.111048495776256	17.790452397788442
9	26.058029658929883	15.005465880026625	19.895603885022293	39.0409005760212
10	16.14602215147088	27.918223575032513	33.7276504134008	22.208103860095804
11	34.901149529806695	22.537851368743304	22.124390746809507	20.436608354640494
12	20.48035932428083	26.45984462622833	30.027682359186286	23.032113690304552
13	29.138066015706478	20.081056946852343	26.29999605164449	24.480880985796688
14	21.810795601611726	21.278365843454484	25.83704139751046	31.07379715742333
15	24.21135586927035	28.671521947472513	23.223868167998937	23.8932540152582
16	24.08768061233809	27.85552847542029	24.278198736286942	23.778592175954675
17	22.734511379040935	27.847950823431532	25.974675487053585	23.44286231047395
18	22.22995940372653	26.56641034261755	27.611647728519053	23.591982525136867
19	23.45718008449481	26.691481482810893	27.582374062415127	22.268964370279175
20	23.75605863188285	25.901571086551545	27.637571274796368	22.704799006769235
21	23.591543818969097	26.75740705511306	26.401775882567136	23.24927324335071
22	23.420607943054346	26.198854338784056	26.830591220372874	23.54994649778872
23	22.842871802480126	26.64697274797168	26.92283916274124	23.58731628680695
24	23.687421057816287	26.724105268741425	26.795215550299055	22.793258123143225
25	23.642633146324858	26.056514128532132	26.981785318737977	23.31906740640503
26	23.459812321501428	26.677961356367803	26.73475386390457	23.1274724582262
27	23.48298398363546	26.15374736826152	26.665996642701344	23.697272005401672
28	22.840279447852392	26.435037786559878	27.41961407417245	23.30506869141528
29	22.864926758005293	26.88100254728754	27.071720083130828	23.18235061157634
30	22.593367640155655	27.530965676028256	26.96607166145603	22.909595022360055
31	23.454587729867075	26.7424112806511	26.787239074521423	23.015761914960397
32	22.48652274711421	26.819025330495304	26.91290845039809	23.7815434719924
33	22.672813338900912	26.40688082706482	27.29912940755125	23.621176426483018
34	23.105577032216587	26.227130945415777	27.288520694766994	23.37877132760064
35	23.5743146312894	26.217359762588178	27.22343265242148	22.984892953700946
36	22.846182039927847	26.878689369312024	27.054131954041143	23.220996636718986
37	23.810218902413002	25.644449389859425	26.98346037865128	23.56187132907629
38	23.103582913272177	26.147087010987196	27.73915169382457	23.010178381916052
39	23.09827855688005	25.89770249579939	27.658270229439335	23.345748717881225
40	22.770206108145857	25.36276014777219	28.47518099620599	23.391852747875962
41	22.091168725195654	26.159011842274765	28.50018724776888	23.249632184760706
42	22.958530701255857	25.396700052206032	28.213472825941732	23.431296420596375
43	22.26665119230366	25.324871887828415	28.324026780219775	24.08445013964815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	457.0
1	419.5
2	382.0
3	798.5
4	1215.0
5	1215.0
6	1667.0
7	2119.0
8	1980.5
9	1842.0
10	2831.5
11	3821.0
12	3821.0
13	6108.0
14	8395.0
15	13388.5
16	18382.0
17	18700.5
18	19019.0
19	19019.0
20	22157.0
21	25295.0
22	28208.0
23	31121.0
24	34978.5
25	38836.0
26	38836.0
27	44640.5
28	50445.0
29	63909.0
30	77373.0
31	82980.5
32	88588.0
33	88588.0
34	97342.0
35	106096.0
36	114019.5
37	121943.0
38	136607.0
39	151271.0
40	151271.0
41	161201.5
42	171132.0
43	163142.5
44	155153.0
45	164172.0
46	173191.0
47	173191.0
48	179115.5
49	185040.0
50	186424.0
51	187808.0
52	191859.0
53	195910.0
54	195910.0
55	174948.5
56	153987.0
57	143616.5
58	133246.0
59	120667.0
60	108088.0
61	108088.0
62	98237.5
63	88387.0
64	77295.5
65	66204.0
66	57383.5
67	48563.0
68	48563.0
69	41515.0
70	34467.0
71	29494.5
72	24522.0
73	19838.5
74	15155.0
75	15155.0
76	11702.5
77	8250.0
78	6736.5
79	5223.0
80	3935.0
81	2647.0
82	2647.0
83	1958.5
84	1270.0
85	1141.5
86	1013.0
87	925.5
88	838.0
89	838.0
90	695.0
91	552.0
92	313.5
93	75.0
94	58.5
95	42.0
96	42.0
97	24.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2507373.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.23550364071028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.09258016641188	46.150389414128625
2	9.01278339976275	9.776256937815315
3	2.3925106157512905	3.892770546330513
4	1.0275543887582563	2.2291971917010494
5	0.5376152573457513	1.4578917123538446
6	0.36148570239921235	1.1763215477122315
7	0.23062539957525263	0.8755659288812714
8	0.17777882629119007	0.7713539344445631
9	0.12853289085093686	0.6273941462727303
>10	0.818048847621531	8.54710909664983
>50	0.1042350751338664	3.9707765800854014
>100	0.09957560918960881	11.447870456604957
>500	0.012320920759913907	4.532569182507625
>1k	0.004057788274222949	3.5945644438066005
>5k	2.9511187448894174E-4	0.9499688807054479
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6778	0.27032276410410416	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6386	0.2546888715799364	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5313	0.2118950790329161	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5264	0.20994084246739514	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3749	0.14951903845179795	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3050	0.12164125560895807	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2879	0.11482136881907877	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2847	0.11354513269465692	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2658	0.10600736308479033	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2521	0.10054347717710926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.988237888818297E-5	0.0
2	0.0	0.0	0.0	3.988237888818297E-5	0.0
3	0.0	0.0	0.0	3.988237888818297E-5	0.0
4	0.0	0.0	0.0	3.988237888818297E-5	0.0
5	0.0	0.0	0.0	3.988237888818297E-5	0.0
6	0.0	0.0	0.0	3.988237888818297E-5	0.0
7	0.0	0.0	0.0	7.976475777636594E-5	0.0
8	0.0	0.0	0.0	7.976475777636594E-5	0.0
9	0.0	0.0	0.0	1.5952951555273188E-4	0.0
10	0.0	0.0	0.0	1.5952951555273188E-4	0.0
11	0.0	0.0	0.0	2.3929427332909783E-4	0.0
12	0.0	0.0	0.0	4.387061677700127E-4	0.0
13	0.0	0.0	0.0	5.184709255463786E-4	0.0
14	0.0	0.0	0.0	5.982356833227445E-4	0.0
15	0.0	0.0	0.0	6.780004410991105E-4	0.0
16	0.0	0.0	0.0	0.0010369418510927572	0.0
17	0.0	0.0	0.0	0.001395883261086404	0.0
18	0.0	0.0	0.0	0.0014756480188627698	0.0
19	0.0	0.0	0.0	0.0018744718077445997	0.0
20	0.0	0.0	0.0	0.0022334132177382463	0.0
21	0.0	0.0	0.0	0.00255247224884371	0.0
22	0.0	0.0	0.0	0.0035894140999364675	0.0
23	0.0	0.0	0.0	0.004945414982134688	0.0
24	0.0	0.0	0.0	0.007099063442096569	0.0
25	3.988237888818297E-5	0.0	0.0	0.008215770050965692	0.0
26	3.988237888818297E-5	0.0	0.0	0.010688477542033037	0.0
27	7.976475777636594E-5	0.0	0.0	0.0201406013385324	0.0
28	7.976475777636594E-5	0.0	0.0	0.04606414761585133	0.0
29	7.976475777636594E-5	0.0	0.0	0.08471017275850062	0.0
30	7.976475777636594E-5	0.0	0.0	0.13918950231975857	0.0
31	7.976475777636594E-5	0.0	0.0	0.29445160333145487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2395	0.0	19.23382	1
TCGCCAT	715	0.0	18.888111	13
ACCGTCG	255	0.0	18.862745	8
ATACCGT	350	0.0	16.914286	6
GTCGCCA	815	0.0	16.57055	12
TCTAGCG	285	0.0	16.22807	28
ATCTCGC	870	0.0	16.16092	11
CGTCGTA	300	0.0	16.033333	10
TACCGTC	325	0.0	15.938462	7
GGTCCGA	70	0.002593639	15.857143	6
CCGTCGT	305	0.0	15.770493	9
TGTTCTA	915	0.0	15.770491	15
CGGCCTT	880	0.0	15.767045	24
AGGTCGC	860	0.0	15.703488	10
TTGGCCG	895	0.0	15.502793	31
TATACTG	375	0.0	15.293334	5
GAGGTCG	940	0.0	15.154256	9
CGCAATA	330	0.0	15.136362	36
TTATACT	395	0.0	14.987342	4
TAGAACA	1210	0.0	14.830578	4
>>END_MODULE